We performed differential cDNA hybridization using RNA from endothelial cells cultured for 4 hours on either plastic or basement membrane matrix (Matrigel), and identified early genes induced during the morphological differentiation into capillary-like tubes. The mRNA for one clone, thymosin beta 4, was increased 5-fold. Immunostaining localized thymosin beta 4 in vivo in both growing and mature vessels as well as in other tissues. Endothelial cells transfected with thymosin beta 4 showed an increased rate of attachment and spreading on matrix components, and an accelerated rate of tube formation on Matrigel. An antisense oligo to thymosin beta 4 inhibited tube formation on Matrigel. The results suggest that thymosin beta 4 is induced and likely involved in differentiating endothelial cells. Thymosin beta 4 may play a role in vessel formation in vivo.
Matrigel induces thymosin beta 4 gene in differentiating endothelial cells
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D.S. Grant, J.L. Kinsella, M.C. Kibbey, S. LaFlamme, P.D. Burbelo, A.L. Goldstein, H.K. Kleinman; Matrigel induces thymosin beta 4 gene in differentiating endothelial cells. J Cell Sci 1 December 1995; 108 (12): 3685–3694. doi: https://doi.org/10.1242/jcs.108.12.3685
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