Analysis by double-label indirect immunofluorescence of PtK1 and HeLa cells had previously demonstrated that prosome* antigens form networks that superimpose on those of the intermediate filaments of the cytokeratin type. We show here that in PtK1 cells various prosomal antigens also reside to a variable extent on intermediate filaments subnetworks of the vimentin type. In proliferating human fibroblasts the prosome and vimentin networks were found to coincide, while in proliferating myoblasts of the C2.7 mouse myogenic cell line the prosomal antigens seem to superimpose on the intermediate filaments of the desmin type. Thus, the prosomes, which are RNP particles of variable composition and subcomplexes of untranslated mRNP, and carry a multicatalytic proteinase activity, seem to co-localize with the specific kind of cytoplasmic intermediate filament in relation to the cell type. These results, which generalize the previous data, are discussed in view of possible role(s) for prosomes in mRNA metabolism and/or intermediate filaments remodelling.

REFERENCES

Agutter
P. S.
(
1988
).
Nucleo-cytoplasmic transport of mRNA.
Prog. Mol. Subcell. Biol
10
,
15
96
Ahn
J. Y.
,
Hong
S. O.
,
Kwak
K. B.
,
Kang
S. S.
,
Tanaka
K.
,
Ishihara
A.
,
Ha
D. B.
,
Chung
C. H.
(
1991
).
Developmental regulation of proteolytic activities and subunit pattern of 20S proteasome in Chick embryonic muscle.
J. Biol. Chem
266
,
15746
15749
Albers
K.
,
Fuchs
E.
(
1989
).
Expression of mutant keratin cDNAs in epithelial cells reveals possible mechanism for initiation and assembly of intermediate filaments.
J. Cell Biol
108
,
1477
1493
Arcangeletti
C.
,
Olink-Coux
M.
,
Minisini
R.
,
Huesca
M.
,
Chezzi
C.
,
Scherrer
K.
(
1992
).
Patterns of cytodistribution of prosomal antigens on the vimentin and cytokeratin networks of Monkey Kidney cells.
Eur. J. Cell Biol
59
,
464
476
Arrigo
A. P.
,
Simon
J. M.
,
Darlix
L.
,
Spahr
P. F.
(
1987
).
A 20S particle ubiquitous from yeast to human.
J. Mol. Evol
25
,
141
150
Arrigo
A. P.
,
Tanaka
K.
,
Goldberg
A. L.
,
Welch
W. J.
(
1988
).
Identity of the 19S ‘prosome’ particle with the large multifunctional complex of mammalian cells (the proteasome).
Nature
331
,
192
194
Bey
F.
,
Silva-Pereira
I.
,
Coux
O.
,
Viegas-Pequignot
E.
,
Recillas Targa
F.
,
Nothwang
H.-G.
,
Dutrillaux
B.
,
Scherrer
K.
(
1993
).
The prosomal RNA-binding protein 27K is a member of the a-type human prosomal gene family.
Mol. Gen. Genet
237
,
193
205
Bradford
M. M.
(
1976
).
A rapid and sensitive method for the quantitation of microgram quantities of proteins utilizing the principle of protein-dye binding.
Anal. Biochem
72
,
248
254
Briane
D.
,
Olink-Coux
M.
,
Vassy
J.
,
Oudar
O.
,
Huesca
M.
,
Scherrer
K.
,
Foucrier
J.
(
1992
).
Immunolocalization of a specific type of prosome close to the bile canaliculi in foetal and adult rat liver.
Eur. J. Cell Biol
57
,
30
39
Brown
M. G.
,
Driscoll
J.
,
Monaco
J. J.
(
1991
).
Structural and serological similarity of MHC-linkeed LMP and proteasome (multicatalytic proteinase) complexes.
Nature
353
,
355
357
Bruckenstein
D. A.
,
Lein
P. J.
,
Higgins
D.
,
Fremeau
R. T.
(
1990
).
Distinct spatial localization of specific messenger RNAs in cultured sympathetic neurons.
Neuron
5
,
809
819
Carmo-Fonseca
M.
,
Citadao
A. J.
,
David-Ferreira
J. F.
(
1987
).
Filamentous cross-bridges link intermediate filaments to the nuclear pore complexes.
Eur. J. Cell Biol
45
,
282
290
Chou
Y. H.
,
Bischoff
J. R.
,
Beach
D.
,
Goldman
R. D.
(
1990
).
Intermediate filament organization during mitosis is mediated by p34cdc2 phosphorylation of vimentin.
Cell
62
,
1063
1071
Coux
O.
,
Nothwang
H. G.
,
Scherrer
K.
,
Bergsma-Schutter
W.
,
Arnberg
A. C.
,
Timmins
P. A.
,
Langowski
J.
,
Cohen-Addad
C.
(
1992
).
Structure and RNA content of prosomes.
FEBS Lett
300
,
49
55
Dahlmann
B.
,
Kuehn
L.
,
Ishiura
S.
,
Tsukahara
T.
,
Sugita
H.
,
Tanaka
K.
,
Rivett
J.
,
Hough
R. F.
,
Rechsteiner
M.
,
Mykles
D. L.
,
Fagan
J. M.
,
Waxman
L.
,
Ishii
S.
,
Sasaki
M.
,
Kloetzel
P. M.
,
Harris
H.
,
Ray
K.
,
Behal
F. J.
,
Demartino
G. N.
,
Mc Guire
M. J.
(
1988
).
The multicatalytic proteinase: a high- M rendopeptidase.
Biochem. J
255
,
750
751
Emori
Y.
,
Tsukahara
T.
,
Kawasaki
H.
,
Ishiura
S.
,
Sugita
H.
,
Suzuki
K.
(
1991
).
Molecular cloning and functional analysis of three subunits of yeast proteasome.
Mol. Cell Biol
11
,
344
353
Eriksson
J. E.
,
Opal
P.
,
Goldman
R. D.
(
1992
).
Intermediate filament dynamics.
Curr. Opin. Cell Biol
4
,
99
104
Eytan
E.
,
Ganoth
D.
,
Armon
T.
,
Hershko
A.
(
1989
).
ATP-dependent incorporation of 20 S protease into the 26 S complex that degrades proteins conjugated to ubiquitin.
Proc. Nat. Acad. Sci. USA
86
,
7751
7755
Falkenburg
P. E.
,
Kloetzel
P. M.
(
1989
).
Identification and characterization of 3 different subpopulations of the Drosophila multicatalytic Protease (Proteasome).
J. Biol. Chem
264
,
6660
6666
Fujiwara
T.
,
Tanaka
K.
,
Orino
E.
,
Yoshimura
T.
,
Kumatori
A.
,
Tamura
T.
,
Chung
C. H.
,
Nakai
T.
,
Yamaguchi
K.
,
Shin
S.
,
Kakizuka
A.
,
Nakanishi
S.
,
Ichihara
A.
(
1990
).
Proteasomes are essential for yeast proliferation- cDNA cloning and gene disruption of 2 major subunits.
J. Biol. Chem
265
,
16604
16613
Gautier
J.
,
Pal
J. K.
,
Grossi de Sa
M. F.
,
Beetschen
J. C.
,
Scherrer
K.
(
1988
).
Differential localization of prosomes in Axolotl during oogenesis and meiotic maturation.
J. Cell Sci
90
,
543
553
Goldberg
A. L.
,
Rock
K.
(
1992
).
Proteolysis, proteasomes and antigen presentation.
Nature
357
,
375
379
Grainger
J. L.
,
Winkler
M. M.
(
1987
).
Fertilization triggers unmasking of maternal mRNAs in sea urchin eggs.
Mol. Cell. Biol
7
,
3947
3954
Grossi de Sa
M. F.
,
Martins de Sa
C.
,
Harper
F.
,
Coux
O.
,
Akhayat
O.
,
Florentin
Y.
,
Pal
J. K.
,
Scherrer
K.
(
1988
).
Cytolocalization of prosomes as a function of differentiation.
J. Cell Sci
89
,
151
165
Grossi de Sa
M. F.
,
Martins de Sa
C.
,
Harper
F.
,
Olink-Coux
M.
,
Huesca
M.
,
Scherrer
K.
(
1988
).
The association of prosomes with some of theintermediate filament networks on the animal cell.
J. Cell Biol
107
,
1517
1530
Haass
C.
,
Kloetzel
P. M.
(
1989
).
The Drosophila proteasome undergoes changes in its subunits pattern during development.
Exp. Cell Res
180
,
243
252
Heinemeyer
W.
,
Kleinschmidt
J. A.
,
Saidowsky
J.
,
Escher
C.
,
Wolf
D. H.
(
1991
).
Proteinase yscE, the yeast proteasome/multicatalytic-multifunctional proteinase- mutants unravel its function in stress induced proteolysis and uncover its necessity for cell survival.
EMBO J
10
,
555
562
Hersko
A.
,
Ciechanover
A.
(
1992
).
The ubiquitin system for protein degradation.
Annu. Rev. Biochem
61
,
767
807
Hesketh
J. E.
,
Pryme
I. F.
(
1991
).
Interaction between mRNA, ribosomes and the cytoskeleton.
Biochem. J
277
,
1
10
Horsch
A.
,
Martins de Sa
C.
,
Dineva
B.
,
Spindler
E.
,
Schmid
H. P.
(
1989
).
Prosomes discriminate between mRNA of adenovirus-infected and uninfected HeLa cells.
FEBS Lett
246
,
131
136
Horsch
A.
,
Koeler
K.
,
Ellwart
T. M.
,
Schmid
H. P.
(
1990
).
Selection of prosomes and prosomal RNA by immobilized viral RNAs.
FEBS Lett
269
,
336
340
Isaacs
W. B.
,
Cook
R. K.
,
Van Atta
J. C.
,
Redmond
C. M.
,
Fulton
A. B.
(
1989
).
Assembly of vimentin in cultured cells varies with the cell type.
J. Biol. Chem
264
,
17953
17960
Kleinschmidt
J. A.
,
Buhl
W. J.
(
1987
).
The 20S cylinder particles of Xenopus laevis: experimental findings and open questions.
Mol. Biol. Rep
12
,
229
–.
Kumatori
A.
,
Tanaka
K.
,
Inamura
N.
,
Sone
S.
,
Ogura
T.
,
Matsumoto
T.
,
Tachikawa
T.
,
Shin
S.
,
Ishihara
A.
(
1990
).
Abnormally high expression of proteasomes in human leukemic cells.
Proc. Nat. Acad. Sci. USA
87
,
7071
7075
Laemmli
U. K.
(
1970
).
Cleavage of structural proteins during the assembly of the head bacteriophage T4.
Nature
227
,
680
685
Martins de Sa
C.
,
Grossi de Sa
M. F.
,
Akhayat
O.
,
Broders
F.
,
Scherrer
K.
,
Horsch
A.
,
Schmid
H. P.
(
1986
).
Prosomes: Ubiquity and inter species structural variation.
J. Mol. Biol
187
,
479
493
Melton
D. A.
(
1987
).
Translocation of localized maternal mRNA to the vegetal pole of Xenopus oocytes.
Nature
328
,
80
83
Monteiro
M. J.
,
Cleveland
D. W.
(
1989
).
Expression of NF-L and NF-M in fibroblasts reveals coassembly of neurofilament and vimentin subunits.
J. Cell Biol
108
,
579
593
Murti
K.
,
Goorha
R.
(
1989
).
Synthesis of frog virus 3 proteins occurs on the intermediate filament-bound polyribosomes. Biol.
Cell
65
,
205
214
Nelson
W. J.
,
Traub
P.
(
1982
).
Purification and further characterization of the Ca2+-activated proteinase specific for the intermediate filament proteins vimentin and desmin.
J. Biol. Chem
257
,
5544
5553
Nothwang
H. G.
,
Coux
O.
,
Keith
G.
,
Silva-Peirera
I.
,
Scherrer
K.
(
1992
).
The major RNA in prosomes of HeLa cells and duck erythroblasts is tRNALys, 3.
Nucl. Acids Res
20
,
1959
1965
Nothwang
H. G.
,
Coux
O.
,
Bey
F.
,
Scherrer
K.
(
1992
).
Prosomes and their Multicatalytic (MCP) Proteinase activity.
Eur. J. Biochem
207
,
621
630
Ohta
M.
,
Marceau
N.
,
Perry
G.
,
Manetto
V.
,
Gambetti
P.
,
Autilio-Gambetti
L.
,
Metuzals
J.
,
Kawahara
H.
,
Cadrin
M.
,
French
S.
(
1988
).
Ubiquitin is present on the cytokeratin intermediate filaments and Mallory bodies of hepatocytes.
Lab. Invest
59
,
848
856
Olink-Coux
M.
,
Huesca
M.
,
Scherrer
K.
(
1992
).
Specific type of prosomes are associated to subnetworks of the intermediate filaments in PtK1cells.
Eur. J. Cell Biol
59
,
148
159
Orlowski
M.
(
1990
).
The multicatalytic protease complex, a major extralysosomal proteolytic system.
Biochemistry
29
,
10289
10297
Orlowski
M.
,
Wilk
S.
(
1988
).
‘Multicatalytic proteinase complex’ or ‘multicatalytic proteinase: a high- M rendopeptidase'.
Biochem. J
255
,
751
–.
Pal
J. K.
,
Gounon
P.
,
Grossi de Sa
M. F.
,
Scherrer
K.
(
1988
).
Presence and distribution of specific prosome antigens change as a function of embryonic development and tissue-type differentiation in Pleurodeles walti.
J. Cell Sci
90
,
555
567
Reynaud
C. A.
,
Imaizumi-Scherrer
M. T.
,
Scherrer
K.
(
1980
).
The size of transcriptional units at the pre-messenger RNA level.
J. Mol. Biol
140
,
481
504
Rivett
A. J.
(
1989
).
The multicatalytic proteinase of mammalian cells.
Arch. Biochem. Biophys
268
,
1
8
Rosevear
E.
,
McReynolds
M.
,
Goldman
R. D.
(
1990
).
Dynamicproperties of intermediate filaments: Disassembly and reassembly during mitosis in Baby Hamster Kidney cells.
Cell Motil. Cytoskel
17
,
150
166
Scherrer
K.
(
1990
).
Prosomes, subcomplexes of untranslated messenger RNP.
Mol. Biol. Rep
14
,
1
9
Schmid
H. P.
,
Akhayat
O.
,
Martins de Sa
C.
,
Puvion
F.
,
Koehler
K.
,
Scherrer
K.
(
1984
).
The prosome: an ubiquitous morphologically distinct RNP particle associated with repressed mRNPs and containing specific ScRNA and a characteristic set of proteins.
EMBO J
3
,
29
34
Singer
R. H.
,
Langevin
G. L.
,
Lawrence
J. B.
(
1989
).
Ultrastructural visualization of cytoskeletal mRNAs and their associated proteins using double-label in situ hybridization.
J. Cell Biol
108
,
2343
2353
Skalli
O.
,
Goldman
R. D.
(
1991
).
Recent insight into the assembly, dynamics and function of intermediate filament networks.
Cell Motil. Cytoskel
19
,
67
79
Spohr
G.
,
Granboulan
N.
,
Morel
C.
,
Scherrer
K.
(
1970
).
Messenger RNA in HeLa cells: an investigation of free and polyribosome-bound messenger ribonucleoprotein particles by kinetic labelling and electron microscopy.
Eur. J. Biochem
17
,
296
318
Tanaka
K.
,
Kumatori
A.
,
Li
K.
,
Ichihara
A.
(
1989
).
Direct evidence for nuclear and cytoplasmic colocalization of proteasomes (multiprotease complexes) in liver.
J. Cell. Physiol
139
,
34
41
Tanaka
K.
,
Yoshimura
T.
,
Fujiwara
T.
,
Kumatory
A.
,
Ishihara
A.
(
1990
).
Possible mechanism of nuclear translocation of proteasomes.
FEBS Lett
1
,
41
46
Towbin
H.
,
Staehelin
T.
,
Gordon
J.
(
1979
).
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
Proc. Nat. Acad. Sci. USA
76
,
4350
4354
Traub
P.
(
1985
).
Are intermediate filaments proteins involved in gene expression?.
Ann. NY Acad. Sci
455
,
68
78
Vikstrom
K. L.
,
Borisy
G. G.
,
Goldman
R. D.
(
1989
).
Dynamic aspects of intermediate filament networks in BHK-21 cells.
Proc. Nat. Acad. Sci. USA
86
,
549
553
Vincent
A.
,
Civelli
O.
,
Maundrell
K.
,
Scherrer
K.
(
1980
).
Identification and characterization of the translationally repressed cytoplasmic globin messenger-ribonucleoprotein particles from duck erythroblasts.
Eur. J. Biochem
112
,
617
633
Yaffe
D.
,
Saxel
O.
(
1977
).
A myogenic cell line with altered serum requirements for differentiation.
Differentiation
7
,
159
166
Yang
Y. J.
,
Waters
B.
,
Fruh
K.
,
Peterson
P. A.
(
1992
).
Proteasomes are regulated by interferon gama: Implications for antigen processing.
Proc. Nat. Acad. Sci. USA
89
,
4928
4932
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