CO2 production was followed by manometry in starved cell-free extracts of fission yeast stimulated by unstarved cell-free extracts from a synchronous culture. The degree of stimulus, measured by the lag time in CO2 production, varied markedly during the cell cycle, with a maximum for cells at about mitosis and a minimum for septated cells. Similar differences in lag time were found with unstarved extracts of cdc13.117 grown at 37 degrees C and 35 degrees C.

REFERENCES

Creanor
J.
,
Mitchison
J. M.
(
1989
).
Nucleoside diphosphokinase, an enzyme with step changes in activity during the cell cycle of the fission yeast Schizosaccharomyces pombe. II. Dissociation of the steps from the DNA-division cycle after induction synchronisation.
J. Cell Sci
9
,
185
189
Das
J.
,
Timm
H.
,
Busse
H. G.
,
Degn
H.
(
1990
).
Oscillatory CO2 evolution in glycolysing yeast extracts.
Yeast
6
,
255
261
Hagan
I. M.
,
Hayles
J.
,
Nurse
P.
(
1988
).
Cloning and sequencing of the cyclin-related cdc 13+ gene and a cytological study of its role in fission yeast mitosis.
J. Cell Sci
91
,
587
595
Murray
A. W.
,
Kirschner
M. W.
(
1989
).
Cyclin synthesis drives the early embryonic cell cycles.
Nature
339
,
275
280
Novak
B.
,
Mitchison
J. M.
(
1986
).
Changes in the rate of CO2 production in synchronous cultures of the fission yeast Schizosaccharomyces pombe: a periodic cell cycle event that persists after the DNA-division cycle has been blocked.
J. Cell Sci
86
,
191
206
Novak
B.
,
Mitchison
J. M.
(
1987
).
Periodic cell cycle changes in the rate of CO2 production in the fission yeast Schizosaccharomyces pombe persist after a block to protein synthesis.
J. Cell Sci
87
,
323
325
Novak
B.
,
Mitchison
J. M.
(
1990
).
CO2 production after induction synchrony of the fission yeast Schizosaccharomycespombe: the origin and nature of entrainment.
J. Cell Sci
96
,
79
91
Nurse
P.
(
1990
).
Universal control mechanism regulating onset of M-phase.
Nature
344
,
503
508
Nurse
P.
,
Bisset
Y.
(
1981
).
Gene required in G1 for commitment to cell cycle and in G2 for control of mitosis in fission yeast.
Nature
2
,
558
560
This content is only available via PDF.