The fungal fatty acid derivative Brefeldin A (BFA), has been used to study the reversible distribution of a Golgi glycoprotein, the JIM 84 epitope, into the cytosol of higher plant cells. Treatment of both maize and onion root tip cells resulted in a rearrangement of the Golgi stacks into either circular formations or a perinuclear distribution. The Golgi cisternae became curved and vesiculated and in cells where the Golgi apparatus was totally dispersed the JIM 84 epitope was associated with large areas in the cytosol which were also vesiculated. On removal of the BFA the Golgi apparatus reformed and the JIM 84 epitope was again located in the cisternal stacks. This mode of BFA action is compared with that so far described for animal cells.
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JOURNAL ARTICLE| 01 December 1992
Redistribution of a Golgi glycoprotein in plant cells treated with Brefeldin A
Online Issn: 1477-9137
Print Issn: 0021-9533
© 1992 by Company of Biologists
J Cell Sci (1992) 103 (4): 1153–1166.
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B. Satiat-Jeunemaitre, C. Hawes; Redistribution of a Golgi glycoprotein in plant cells treated with Brefeldin A. J Cell Sci 1 December 1992; 103 (4): 1153–1166. doi: https://doi.org/10.1242/jcs.103.4.1153
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