The fungal fatty acid derivative Brefeldin A (BFA), has been used to study the reversible distribution of a Golgi glycoprotein, the JIM 84 epitope, into the cytosol of higher plant cells. Treatment of both maize and onion root tip cells resulted in a rearrangement of the Golgi stacks into either circular formations or a perinuclear distribution. The Golgi cisternae became curved and vesiculated and in cells where the Golgi apparatus was totally dispersed the JIM 84 epitope was associated with large areas in the cytosol which were also vesiculated. On removal of the BFA the Golgi apparatus reformed and the JIM 84 epitope was again located in the cisternal stacks. This mode of BFA action is compared with that so far described for animal cells.

REFERENCES

Bednarek
S.Y.
,
Raikhel
N.V.
) (
1991
).
The barley lectin carboxyl-terminal propeptide is a vacuolar protein sorting determinant in plants.
Plant Cell
3
,
1195
1206
Brummel
D.A.
,
Camirand
A.
,
MacLachlan
G.A.
) (
1990
).
Differential distribution of xyloglucan glycosyl transferases in pea Golgi dictyosomes and secretory vesicles.
J. Cell Sci
96
,
705
710
Chrispeels
M.J.
,
Raikhel
N.
(
1992
).
Short peptide domains target proteins to plant vacuoles.
Cell
68
,
613
616
Craig
S.
,
Goodchild
D.J.
) (
1984
).
A Golgi-mediated vicilin-accumulation in pea cotyledon cells is re-directed by monensin and nigericin.
Protoplasma
122
,
91
97
Craig
S.
,
Goodchild
D.J.
) (
1984
).
Periodate acid treatment of sections permits on-grid immunogold localization of pea seed vicilin in ER and Golgi.
Protoplasma
122
,
35
44
Denecke
J.
,
Botterman
J.
,
Deblaere
R.
(
1990
).
Protein secretion in plant cells can occur via a default pathway.
Plant Cell
2
,
51
59
Doms
R.W.
,
Russ
G.
,
Yewdell
J.W.
) (
1989
).
Brefeldin A redistributes resident and itinierant Golgi proteins to the endoplasmic reticulum.
J. Cell Biol
109
,
61
72
Donaldson
J.
,
Lippincott-Schwartz
J.
,
Bloom
G.
,
Kreis
T.
,
Klausner
R.D.
) (
1990
).
Dissociation of a 110-kD peripheral membrane protein from the Golgi apparatus is an early event in Brefeldin A action.
J. Cell Biol
111
,
2295
2306
Duden
R.
,
Allan
V.
,
Kreis
T.
(
1991
).
Involvement of-COP in membrane traffic through the Golgi complex.
Trends Biochem. Sci
1
,
14
19
Duden
R.
,
Griffiths
G.
,
Frank
R.
,
Argos
P.
,
Kreis
T.
(
1991
).
-COP, a 110kD protein associated with non-clathrin-coated vesicles and the Golgi complex, shows homology to -adaptin.
Cell
64
,
649
665
Fontes
E.B.P.
,
Shank
B.B.
,
Wrobel
R.L.
,
Moose
S.P.
,
O'Brian
G.R.
,
Wurtzel
E.T.
,
Boston
R.S.
) (
1991
).
Characterization of an immunoglobulin binding protein in the maize floury-2 endosperm mutant.
Plant Cell
3
,
483
496
Fujiwara
T.
,
Oda
K.
,
Yokota
S.
,
Takatsuki
A.
,
Ikehara
Y.
(
1988
).
Brefeldin A causes diassembly of the Golgi complex and accumulation of secretory proteins in the endoplasmic reticulum.
J. Biol. Chem
263
,
18545
18552
Greenwood
J.S.
,
Chrispeels
M.J.
) (
1985
).
Immunocytochemical localization of phaseolin and phytohemaglutinin in the endoplasmic reticulum and Golgi complex of developing bean cotyledons.
Planta
165
,
295
302
Griffing
L.
(
1991
).
Comparisons of Golgi structure and dynamics in plant and animal cells.
J. Elect. Microsc. Techn
17
,
179
199
Herman
E.M.
,
Shannon
L.M.
) (
1984
).
Immunocytochemical localization of concanavalin A in developing jack-bean cotyledons.
Planta
161
,
97
104
Höfte
H.
,
Faye
L.
,
Dickinson
C.
,
Herman
E.M.
,
Chrispeels
M.J.
) (
1991
).
The protein-body proteins phytohemagglutinin and tonoplast intrinsic protein are targeted to vacuoles in leaves of transgenic tobacco.
Planta
184
,
431
437
Hrazdina
G.
,
Zobel
A.M.
) (
1991
).
Cytochemical localization of enzymes in plant cells.
Int. Rev. Cytol
129
,
269
322
Hunziker
W.
,
Whitney
J.A.
,
Mellman
I.
(
1991
).
Selective inhibition of transcytosis by Brefeldin A in MDCK cells.
Cell
67
,
617
627
Klausner
R.D.
,
Donaldson
J.G.
,
Lippincott-Schwartz
J.
(
1992
).
Brefeldin A: insights into the control of membrane traffic and organelle structure.
J. Cell Biol
116
,
1071
1092
Laine
A.C.
,
Gomord
V.
,
Faye
L.
(
1991
).
Xylose-specific antibodies1166as markers of subcompartmentation of terminal glycosylation in the Golgi apparatus of sycamore cells.
FEBSLett
295
,
179
184
Lippincott-Schwartz
J.
,
Yuan
L.
,
Bonifacino
J.
,
Klausner
R.
(
1989
).
Rapid redistribution of Golgi proteins into the ER in cells treated with Brefeldin A: evidence for membrane cycling from Golgi to ER.
Cell
56
,
801
813
Lippincott-Schwartz
J.
,
Donaldson
J.
,
Schweizer
A.
,
Berger
E.
,
Hauri
H.
,
Yuan
L.
,
Klausner
R.
(
1990
).
Microtubule-dependent retrograde transport of proteins into the ER in the presence of Brefeldin A suggests an ER recycling pathway.
Cell
60
,
821
836
Lippincott-Schwartz
J.
,
Yuan
L.
,
Tipper
C.
,
Amherd
T.M.
,
Orci
L.
,
Klausner
R.
(
1991
).
Brefeldin A's effects on endosomes, lysosomes, and the TGN suggest a general mechanism for regulating organelle structure and membrane traffic.
Cell
67
,
601
616
Matsuoka
K.
,
Nakamura
K.
(
1991
).
Propeptide of a precursor to a plant vacuolar protein required for vacuolar targeting.
Proc. Natl. Acad. Sci. U.S.A
88
,
834
838
Misumi
Y.
,
Misumi
Y.
,
Miki
K.
,
Takatsuki
G.
,
Ikehara
Y.
(
1986
).
Novel blockade by Brefeldin A of intracellular transport of secretory proteins in cultured rat hepatocytes.
J. Biol. Chem
261
,
11398
11403
Moore
P. J.
,
Staehelin
L.A.
) (
1988
).
Immunogold localization of the cell-wall-matrix polysaccharides rhamnogalactorunan I and xyloglucan during cell expansion and cytokinesis in Trifolium pratense L; implication for secretory pathways.
Planta
174
,
433
445
Moore
P.J.
,
Swords
K.M.
,
Lynch
M.A.
,
Staehelin
L.A.
) (
1991
).
Spatial organization of the assembly pathways of glycoproteins and complex polysaccharides in the Golgi apparatus of plants.
J. Cell Biol
112
,
589
602
Napier
R.M.
,
Fowke
L.C.
,
Hawes
C.
,
Lewis
M.
,
Pelham
H.R.B.
(
1992
).
Immunological evidence that plants use both HDEL and KDEL for targeting proteins to the endoplasmic reticulum.
J. Cell Sci
102
,
261
271
Narula
N.
,
McMorrow
I.
,
Plopper
G.
,
Doherty
J.
,
Matlin
K.
,
Burke
B.
,
Stow
J.
(
1992
).
Identification of a 200-kD, Brefeldin-sensitive protein on Golgi membranes.
J. Cell Biol
117
,
27
38
Neuhaus
J.M.
,
Sticher
L.
,
Meins
F.
Jr.
,
Boller
T.
(
1991
).
A short C-terminal sequence is necessary and sufficient for the targeting of chitinases to the plant vacuole.
Proc. Natl. Acad. Sci. U.S.A
88
,
10362
10366
Orci
L.
,
Tagaya
M.
,
Amherd
T.M.
,
Perrelet
A.
,
Donaldson
J.
,
Lippincott-Schwartz
J.
,
Klausner
R.
,
Rothman
J.
(
1991
).
Brefeldin A, a drug that blocks secretion, prevents the assembly of non-clathrin-coated buds on Golgi cisternae.
Cell
64
,
1183
1195
Pelham
H.R.
(
1991
).
Multiple targets for Brefeldin A.
Cell
67
,
449
451
Pelham
H.R.
(
1991
).
Recycling of proteins between the endoplasmic reticulum and Golgi complex.
Curr. Opin. Cell Biol
3
,
585
591
Reaves
B.
,
Banting
G.
(
1992
).
Perturbation of the morphology of the trans -Golgi network following Brefeldin A treatment: redistribution of a TGN-specific integral membrane protein, TGN38.
J. Cell Biol
116
,
85
94
Satiat-Jeunemaitre
B.
,
Hawes
C.
(
1992
).
Reversible dissociation of the plant Golgi apparatus by Brefeldin A. Biol.
Cell
74
,
325
328
Serafini
T.
,
Stenbeck
G.
,
Brecht
A.
,
Lottspeich
F.
,
Orci
L.
,
Rothman
J.E.
,
Wieland
F.T.
(
1991
).
A coat subunit of Golgi-derived, non-clathrin-coated vesicles with homology to the clathrin-coated vesicle coat protein β-adaptin.
Nature
349
,
215
220
Vian
B.
,
Roland
J.C.
) (
1991
).
Affinodetection of the sites of formation and further distribution of polygalacturonans and native cellulose in growing plant cells. Biol.
Cell
71
,
43
55
Waters
M.G.
,
Serafini
T.
,
Rothman
J.E.
) (
1991
).
‘Coatomer’: a cytosolic protein complex containing subunits of non-clathrin-coated, Golgi transport vesicles.
Nature
349
,
248
251
Wood
S.A.
,
Park
J.E.
,
Brown
W.J.
) (
1991
).
Brefeldin A causes a microtubule-mediated fusion of the trans-Golgi network and early endosomes.
Cell
67
,
591
600
Zingen-Sell
I.
,
Hillmer
S.
,
Robinson
D.G.
,
Jones
R.L.
) (
1990
).
Localization of-amylase isozymes within the endomembrane system of barley aleurone.
Protoplasma
154
,
16
24
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