We examined the combined effects of IGF-I and EGF on hyaluronic acid synthesis using cultured pericardial mesothelial cells from rabbit pericardial cavity. Combined exposure of pericardial cells to IGF-I and EGF cooperatively increased hyaluronic acid synthesis and the level of hyaluronic acid synthase activity over that seen with or without IGF-I or EGF alone, but did not significantly stimulate the sulfated glycosaminoglycan synthesis. Increase in the level of hyaluronic acid synthesis and hyaluronic acid synthase activity induced by these growth factors was blocked after pretreatment with a tyrosine-specific protein kinase inhibitor, genistein. Genistein had no direct inhibitory effect on hyaluronic acid synthase activity. These results suggest that cooperative enhancement of hyaluronic acid synthesis induced by combined use of IGF-I and EGF could be mediated by a receptor tyrosine kinase-involved transmembrane signaling process that is responsive to IGF-I and EGF.
JOURNAL ARTICLE|
01 January 1991
Cooperative enhancement of hyaluronic acid synthesis by combined use of IGF-I and EGF, and inhibition by tyrosine kinase inhibitor genistein, in cultured mesothelial cells from rabbit pericardial cavity
ATSUSHI HONDA,
ATSUSHI HONDA
1 Department of Biochemistry, Tokyo College of Pharmacy, 1432-1, Horinouchi, Hachioji-shi, Tokyo 192-03, Japan
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NAKAE NOGUCHI,
NAKAE NOGUCHI
1 Department of Biochemistry, Tokyo College of Pharmacy, 1432-1, Horinouchi, Hachioji-shi, Tokyo 192-03, Japan
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HIROMI TAKEHARA,
HIROMI TAKEHARA
1 Department of Biochemistry, Tokyo College of Pharmacy, 1432-1, Horinouchi, Hachioji-shi, Tokyo 192-03, Japan
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YOSHIAKI OHASHI,
YOSHIAKI OHASHI
2 Department of Biochemistry, Tokyo College of Pharmacy, 1432-1, Horinouchi, Hachioji-shi, Tokyo 192-03, Japan; Present address: International Development Department, Chugai Pharmaceutical Co., Ltd, Tokyo, Japan
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NORIKO ASUWA,
NORIKO ASUWA
3 Department of Pathology, Tokyo Medical College, Shinjuku, Shinjuku-ku, Tokyo 160, Japan
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Y.O. MORI
Y.O. MORI
1 Department of Biochemistry, Tokyo College of Pharmacy, 1432-1, Horinouchi, Hachioji-shi, Tokyo 192-03, Japan
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ATSUSHI HONDA
1 Department of Biochemistry, Tokyo College of Pharmacy, 1432-1, Horinouchi, Hachioji-shi, Tokyo 192-03, Japan
NAKAE NOGUCHI
1 Department of Biochemistry, Tokyo College of Pharmacy, 1432-1, Horinouchi, Hachioji-shi, Tokyo 192-03, Japan
HIROMI TAKEHARA
1 Department of Biochemistry, Tokyo College of Pharmacy, 1432-1, Horinouchi, Hachioji-shi, Tokyo 192-03, Japan
YOSHIAKI OHASHI
2 Department of Biochemistry, Tokyo College of Pharmacy, 1432-1, Horinouchi, Hachioji-shi, Tokyo 192-03, Japan; Present address: International Development Department, Chugai Pharmaceutical Co., Ltd, Tokyo, Japan
NORIKO ASUWA
3 Department of Pathology, Tokyo Medical College, Shinjuku, Shinjuku-ku, Tokyo 160, Japan
Y.O. MORI
1 Department of Biochemistry, Tokyo College of Pharmacy, 1432-1, Horinouchi, Hachioji-shi, Tokyo 192-03, Japan
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Online ISSN: 1477-9137
Print ISSN: 0021-9533
© 1991 by Company of Biologists
1991
J Cell Sci (1991) 98 (1): 91–98.
Citation
ATSUSHI HONDA, NAKAE NOGUCHI, HIROMI TAKEHARA, YOSHIAKI OHASHI, NORIKO ASUWA, Y.O. MORI; Cooperative enhancement of hyaluronic acid synthesis by combined use of IGF-I and EGF, and inhibition by tyrosine kinase inhibitor genistein, in cultured mesothelial cells from rabbit pericardial cavity. J Cell Sci 1 January 1991; 98 (1): 91–98. doi: https://doi.org/10.1242/jcs.98.1.91
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