The effect of chemoattractants such as cyclic AMP and folate on amoebae of the cellular slime mould Dictyostelium discoideum is to cause a series of rapid intracellular responses. One of the most rapid of these responses is the polymerization of actin associated with the cytoskeleton, an event correlated with pseudopodium formation, which occurs within 3–5 s of chemotactic receptor stimulation. We report that this response can be mimicked by addition of 5 microM-inositol 1,4,5-triphosphate (IP3) or by addition of 100 microM-Ca2+ to saponin-permeabilized amoebae. The data suggest that cytoskeletal actin polymerization occurs in normal cells as a result of IP3 formation in response to cell surface receptor stimulation and the consequent release of Ca2+ from internal stores.
JOURNAL ARTICLE| 01 June 1986
Inositol 1,4,5-trisphosphate and calcium stimulate actin polymerization in Dictyostelium discoideum
Online Issn: 1477-9137
Print Issn: 0021-9533
© 1986 by Company of Biologists
J Cell Sci (1986) 82 (1): 41–51.
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G.N. Europe-Finner, P.C. Newell; Inositol 1,4,5-trisphosphate and calcium stimulate actin polymerization in Dictyostelium discoideum. J Cell Sci 1 June 1986; 82 (1): 41–51. doi: https://doi.org/10.1242/jcs.82.1.41
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