We have investigated the formation of the prestalk-prespore pattern in Dictyostelium discoideum. Pattern formation occurs in clumps of Dictyostelium cells embedded in agar under a 100% oxygen atmosphere. Agar embedding allows us to control spatially the environment surrounding the cell clumps. Our results suggest that the ambient oxygen concentration plays a role in controlling the size of the multicellular mass. Further, oxygen gradients established across clumps embedded in agar or held in holes in a plastic barrier cause orientation of the prestalk-prespore pattern such that the anterior prestalk region forms at the highest end of the gradient. The results also indicate that developing cells have the ability to migrate up a gradient of oxygen.
JOURNAL ARTICLE| 01 August 1981
Oxygen gradients cause pattern orientation in Dictyostelium cell clumps
Online Issn: 1477-9137
Print Issn: 0021-9533
© 1981 by Company of Biologists
J Cell Sci (1981) 50 (1): 9–17.
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J. Sternfeld, C.N. David; Oxygen gradients cause pattern orientation in Dictyostelium cell clumps. J Cell Sci 1 August 1981; 50 (1): 9–17. doi: https://doi.org/10.1242/jcs.50.1.9
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