The problem of sex-determination in Dinophilus gyro-ciliatus is concerned, first, with the investigation of the factors involved in sex-determination in the sexual or fertilised egg; and secondly, with those involved in sex-determination of the parthenogenetic egg. In these two conditions the factors seem different, and for this reason are perhaps better considered separately. In the present paper only those concerned in sex-determination in the sexual egg are dealt with; while those involved in the parthenogenetic cycle, if it really exists, are left for a future paper.

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