Preliminary.—1. The uses of gases and vapours as a means of microchemical research.—2. Opinions and doubts concerning the red bloodcorpuscle (bibliography).—3. The normal appearance of the frog’s red blood-corpuscle.—4. The normal appearance of the human red bloodcorpuscle.—5. Means of studying the changes of the blood-corpuscles in disease.—6. Effect of pressure on the red blood-corpuscle.—7. Effect of isolation: a, by adhesion; b, by oil.—8. Effect of water in minute quantities gradually added.—9. Effect of CO2 gas.—10. Effect of osmic acid (vapour).—11. Effect of acetic acid (vapour and liquid). 12. Effect of alcohol.—13. Effect of ammonia gas.—14. Effect of chloroform (vapour and liquid).—15. Effect of bisulphide of carbon.—16. Effect of benzine.—17. Effect of turpentine oil.—18. Effect of solution of acetate of rosanilin and of tannin (Robert’s experiments).—19. Effect of carbonic oxide.—20. Effect of cyanogen gas.—21. Effect of sulphuretted hydrogen.—General conclusions and summary.

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To be bought of Messrs. Hopkin and Williams.

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This method is due to Professor Schweigger Seidel, of Leipzig.

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‘Proceedings Royal Society,’ vol. xvii.

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Brucke distinguishes the envelope of the frog’s red corpuscle as “oecoid “—its contents as “zooid.” Stricker divides Briike’s zooid into a “body” and a nucleus. Rollett distinguishes colouring matter and stroma. We thus get the tabular statement:
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I have to thank Dr. Burdon Sanderson for allowing me to make some of these observations in his laboratory.

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I may take this opportunity of mentioning that in a specimen of the annelid Tubifex rivulorum, mounted in glycerine jelly, I obtained crystals of Hæmoglobin in the drops of the red vascular fluid of the worm expressed in the preparation. The crystals in one and the same preparation exhibited three of the forms seen in. different mammalia, viz. rhomboid prisms (dog, man), sphenoids (guinea-pig) and hexahedral plates.

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I was led to believe that the absorption spectrum of blood treated with cyanogen gas was at first identical with that of CO hæmoglobin. Preyer however, whilst confirming me on other points, and working with a large spectroscope, states (Die Blutkrystalle) that the spectrum is that of OHb.

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