CLASP1 regulates Dynein for PLK1-mediated spindle organization and cytokinesis in oocyte meiosis Available to Purchase

The meiotic spindle organization and cytokinesis are important for mammalian oocyte maturation. CLIP-associating protein (CLASP) 1 is a member of microtubule plus-end binding proteins, which is reported to regulate cytokinesis in mitosis; however, the functions of CLASP1 in meiosis are still unclear. In this study, we reported that CLASP1 played critical roles both at metaphase and telophase in mouse oocyte meiosis. Our results indicated that CLASP1 was essential for oocyte maturation and its depletion caused the spindle organization and microtubule-kinetochore attachment defects at metaphase Ⅰ, while this might be due to its association with PLK1/p-MAPK. Besides, deacetylases HDAC6/ SIRT1 were found to be decreased which further affected tubulin acetylation level and microtubule stability after CLASP1 depletion. We also showed that CLASP1 could associate with PLK1/PRC1-based central spindle formation and cytokinesis at telophase Ⅰ. Moreover, Dynein was recognized to interact closely with CLASP1 and may function as a downstream motor protein involved in the orderly transport of PLK1. Taken together, we demonstrated that CLASP1 may play multiple roles in Dynein-based PLK1 for spindle organization and cytokinesis in mouse oocyte meiosis.