Regulation of the distinctive stacked Golgi morphology remains an unresolved subject. Using budding yeast Pichia pastoris, we previously documented the role of GRIP domain Golgin PpImh1 in cisternal stacking, regulated by the Arl3-Arl1 GTPase cascade switch. Expanding our work with the present study, we demonstrate the participation of PpSgm1, another conserved Golgin, in this stacking process alongside PpImh1. Null mutation of P.pastoris SGM1 caused partial unstacking of the late cisternae from the Golgi stack, implicating its role in cisternal stacking. When we overexpressed PpImh1 or PpSgm1 independently, each failed to restore stacking in the absence of the other, suggesting neither of them is sufficient for cisternal stacking alone. On the other hand, a double knock-out of PpIMH1 and PpSGM1 led to a dramatic phenotype, causing complete separation of the late cisternae from the Golgi stack and significantly increasing TGN peeling, as seen in electron microscopy and live-cell imaging. Our results suggest a synergistic collaboration of PpImh1 and PpSgm1 in cisternal stacking, with implications for a conserved mechanism across species.
Intra-Golgi Golgin PpSgm1 and GRIP domain Golgin PpImh1 synergistically mediate Golgi cisternal stacking
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Interviews with Biologists @ 100 conference speakers
Explore our interviews with keynote speakers from the Biologists @ 100 conference, hosted to celebrate our publisher’s 100th anniversary, where we discuss climate change and biodiversity with Hans-Otto Pörtner and Jane Francis, health and disease with Charles Swanton and Sadaf Farooqi, and emerging technologies with Manu Prakash and Jennifer Lippincott-Schwartz.
Introducing our new Associate Editors
In this Editorial, JCS Editor-in-Chief Michael Way welcomes five new Associate Editors to the JCS team. These Associate Editors will expand our support for the wider cell biology community and handle articles in immune cell biology, proteostasis, imaging and image analysis, plant cell biology, and stem cell biology and modelling.
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JCS-FocalPlane Training Grants
Early-career researchers - working in an area covered by JCS - who would like to attend a microscopy training course, please apply. Deadline dates for 2025 applications: 6 June 2025 (decision by week commencing 28 July 2025) and 5 September 2025 (decision by week commencing 20 October 2025).
The emerging roles of the endoplasmic reticulum in mechanosensing and mechanotransduction
In their Review, Jonathan Townson and Cinzia Progida highlight recently emerging evidence for a role of the endoplasmic reticulum in enabling a cell to sense and respond to changes in the extracellular mechanical environment.
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