Firmly united conjugant pairs of P. caudatum were easily separated by treatment with trypsin, 0 · 025–1 · 0 mg/ml in 2 mM phosphate buffer at pH 7 · 2. Cytological observations showed that pairs separated by this means undergo normal meiosis and subsequent prezygotic divisions. Microspectrophotometric comparisons of G1 micronuclei in the parent with those in clones derived from prematurely separated conjugants indicate usually the same DNA content in both. The stock dm-13, heterozygous for mating type gene loci, showed the definite ratio of segregation to 2 mating types in clones derived from prematurely separated conjugants. Those results suggest that the prematurely separated cells usually undergo autogamy.

© 1979 by Company of Biologists
1979
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