In yeast, Arl3p recruits Arl1p GTPase to regulate Golgi function and structure. However, the molecular mechanism involved in regulating activation of Arl1p at the Golgi is unknown. Here, we show that Syt1p promoted activation of Arl1p and recruitment of a golgin protein, Imh1p, to the Golgi. Deletion of SYT1 resulted in the majority of Arl1p being distributed diffusely throughout the cytosol. Overexpression of Syt1p increased Arl1p-GTP production in vivo and the Syt1-Sec7 domain promoted nucleotide exchange on Arl1p in vitro. Syt1p function required the N-terminal region, Sec7 and PH domains. Arl1p, but not Arl3p, interacted with Syt1p. Localization of Syt1p to the Golgi did not require Arl3p. Unlike arl1Δ or arl3Δ mutants, syt1Δ did not show defects in Gas1p transport, cell wall integrity or vacuolar structure. These findings reveal that activation of Arl1p is regulated in part by Syt1p, and imply that Arl1p activation, by using more than one GEF, exerts distinct biological activities at the Golgi compartment.
Syt1p promotes activation of Arl1p at the late Golgi to recruit Imh1p Available to Purchase
These authors contributed equally to this work
Kuan-Yu Chen, Pei-Chin Tsai, Jia-Wei Hsu, Hsin-Chia Hsu, Chiung-Ying Fang, Lin-Chun Chang, Yueh-Tso Tsai, Chia-Jung Yu, Fang-Jen S. Lee; Syt1p promotes activation of Arl1p at the late Golgi to recruit Imh1p. J Cell Sci 15 October 2010; 123 (20): 3478–3489. doi: https://doi.org/10.1242/jcs.074237
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