Multivesicular bodies are late endosomal compartments containing lumenal vesicles that are formed by inward budding of the limiting endosomal membrane. In the yeast Saccharomyces cerevisiae, integral membrane proteins are sorted into the lumenal vesicles of multivesicular bodies, and this process requires the class E subset of VPS genes. We show that one of the class E VPS genes, BRO1/VPS31, encodes a cytoplasmic protein that associates with endosomal compartments. The dissociation of Bro1 from endosomes requires another class E Vps protein, Vps4, which is an ATPase that also regulates the endosomal dissociation of ESCRT-III, a complex of four class E Vps proteins (Vps2, Vps20, Vps24 and Snf7/Vps32) that oligomerize at the endosomal membrane. We also show that the endosomal association of Bro1 is specifically dependent on one of the ESCRT-III components, Snf7. Our data suggest that the function of Bro1 in the MVB pathway takes place on endosomal membranes and occurs in concert with or downstream of the function of the ESCRT-III complex.
Interviews with Biologists @ 100 conference speakers
Explore our interviews with keynote speakers from the Biologists @ 100 conference, hosted to celebrate our publisher’s 100th anniversary, where we discuss climate change and biodiversity with Hans-Otto Pörtner and Jane Francis, health and disease with Charles Swanton and Sadaf Farooqi, and emerging technologies with Manu Prakash and Jennifer Lippincott-Schwartz.
Introducing our new Associate Editors
In this Editorial, JCS Editor-in-Chief Michael Way welcomes five new Associate Editors to the JCS team. These Associate Editors will expand our support for the wider cell biology community and handle articles in immune cell biology, proteostasis, imaging and image analysis, plant cell biology, and stem cell biology and modelling.
The spatial choreography of mRNA biosynthesis
In their Review, André Ventura-Gomes and Maria Carmo-Fonseca detail the latest research progress and technological advancements that are helping to unlock how nuclear organisation underpins control of gene transcription and pre-mRNA splicing.
JCS-FocalPlane Training Grants
Early-career researchers - working in an area covered by JCS - who would like to attend a microscopy training course, please apply. Deadline dates for 2025 applications: 6 June 2025 (decision by week commencing 28 July 2025) and 5 September 2025 (decision by week commencing 20 October 2025).
The emerging roles of the endoplasmic reticulum in mechanosensing and mechanotransduction
In their Review, Jonathan Townson and Cinzia Progida highlight recently emerging evidence for a role of the endoplasmic reticulum in enabling a cell to sense and respond to changes in the extracellular mechanical environment.
Social media
