CO2 production was followed by manometry in starved cell-free extracts of fission yeast stimulated by unstarved cell-free extracts from a synchronous culture. The degree of stimulus, measured by the lag time in CO2 production, varied markedly during the cell cycle, with a maximum for cells at about mitosis and a minimum for septated cells. Similar differences in lag time were found with unstarved extracts of cdc13.117 grown at 37°C and 35°C.

Keywords:
cdc mutants, cell-free extracts, cell cycle, CO2 production, fission yeast, induction synchrony, Schizosaccharomyces pombe
© 1993 by Company of Biologists
1993
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