... cell Spatial transcriptome Xenograft model scRNA-seq Japan Society for the Promotion of Science http://dx.doi.org/10.13039/501100001691 18K16269 21K15562 20J01794 Japan Agency for Medical Research and Development http://dx.doi.org/10.13039/100009619 JP19am0101004...

... phenotypes of murine Mab21l1 -null lens ectoderms using electron microscopy and single-cell RNA sequencing (scRNA-seq). Electron microscopy and immunohistochemical analyses indicated endoplasmic reticulum stress at the 24- to 26-somite stage in Mab21l1 -null lens placodes. scRNA-seq analysis revealed...

... is facilitated by rapidly growing single-cell RNA sequencing (scRNA-seq) data; however, existing computational methods are often not readily adaptable by bench scientists without advanced programming skills. Here, we describe a quantitative intuitive algorithm, coupled with an optimized experimental protocol...