We found the most prominent alcohol-induced DNA methylation changes in the in vitro -cultured ectodermal cells (blue) compared to the endodermal (yellow) and mesodermal cells (green). Horizontal dashed lines indicate FDR<0.05. More about this image found in We found the most prominent alcohol-induced DNA methylation changes in the ...

Fig. 1. Differential gene expression in 70 mM EtOH-exposed germ layer cells. (A-C) Volcano plots showing the effect of 70 mM EtOH exposure on (A) mRNA expression in endodermal cells (control n =4, EtOH n =3), (B) mesodermal cells (control and EtOH n =3) and (C) ectodermal cells (control and E... More about this image found in Differential gene expression in 70 mM EtOH-exposed germ layer cells. (A-C)...

Fig. 2. Gene expression pathways and common genes of 70 mM EtOH-exposed germ layer cells. (A) Significantly enriched terms identified in GO:BP enrichment analysis of 70 mM EtOH-induced DEGs in endodermal cells (FDR-corrected P -value<0.05). The ten most significant pathways are shown. (B) Ve... More about this image found in Gene expression pathways and common genes of 70 mM EtOH-exposed germ layer ...

Fig. 3. Differential DNAm of 70 mM EtOH-exposed germ layer cells. (A-C) Volcano plots showing the effect of 70 mM EtOH exposure on DNAm in (A) endodermal cells, (B) mesodermal cells and (C) ectodermal cells Horizontal dashed lines indicate FDR<0.05. Control and EtOH n =4/germ layer. Common s... More about this image found in Differential DNAm of 70 mM EtOH-exposed germ layer cells. (A-C) Volcano pl...

Fig. 4. DNAm pathways and common DMPs of 70 mM EtOH-exposed germ layer cells. (A) Significantly enriched terms identified in GO:BP enrichment analysis of 70 mM EtOH-induced DMPs in endodermal cells ( P <0.05). The ten most significant pathways are shown. (B) Significantly enriched terms ident... More about this image found in DNAm pathways and common DMPs of 70 mM EtOH-exposed germ layer cells. (A) ...

Fig. 5. Altered metabolites in 70 mM EtOH-exposed germ layers. (A-C) Volcano plots showing the effect of 70 mM EtOH exposure on extracellular metabolites in (A) endodermal cells, (B) mesodermal cells and (C) ectodermal cells. Horizontal line marks P -value <0.05. (D) Venn diagram showing the... More about this image found in Altered metabolites in 70 mM EtOH-exposed germ layers. (A-C) Volcano plots...

Fig. 6. Schematic of EtOH-induced alterations in metabolites of the methionine metabolism. Arrowheads indicate significant EtOH-induced alterations in both exposures (20 and 70 mM EtOH) or in 70 mM EtOH exposure only. Arrowheads annotated with an asterisk indicate significant EtOH-induced altera... More about this image found in Schematic of EtOH-induced alterations in metabolites of the methionine meta...

Fig. 1. A Ldh + cell population arises in fly scrib and dlg mutant tumors over time. (A) Uniform manifold approximation and projection plot of scrib mutant wing disc single cells from different times (left) and expression of the Ldh gene (right). (B) Control and dlg mutant imaginal di... More about this image found in A Ldh + cell population arises in fly scrib and dlg mutant tumors over...

Fig. 2. Systemic depletion of acetyl-CoA leads to a reduction in histone acetylation levels and stochastic silencing of actively transcribed genes in dlg mutant tumors. (A) Plot of metabolic gene expression changes from the scrib mutant tumor time-series transcriptomics data (color coded in ... More about this image found in Systemic depletion of acetyl-CoA leads to a reduction in histone acetylatio...

Fig. 3. Systemic depletion of S-adenosyl methionine leads to a reduction in H3K9me3 levels and transposon activation in fly tumors. (A) Plot of gene expression changes from the scrib mutant tumor time-series transcriptomics data (color coded in squares) and metabolite concentration changes bet... More about this image found in Systemic depletion of S-adenosyl methionine leads to a reduction in H3K9me3...

Fig. 4. The metabolic gene expression signatures of fly and human tumors. (A) Schematic view of using gene expression data from fly and human tumor samples to identify human tumor samples that metabolically resemble fly tumors. RNA-seq, RNA-sequencing; ssGSEA, single-sample gene-set enrichment a... More about this image found in The metabolic gene expression signatures of fly and human tumors. (A) Sche...

Fig. 1. fzd5 is expressed in the optic primordium throughout eye formation. (A-D) Dorsal (A-C) or ventral (D) views, with anterior to the left, at the stage indicated in each panel. Dotted outlines in A and B highlight the optic vesicles. Brackets in D highlight the ciliary marginal zone (CMZ)... More about this image found in fzd5 is expressed in the optic primordium throughout eye formation. (A-D)...

Fig. 2. The Fzd5 sgu3 protein fails to localise to the cell membrane. (A-K) Cell membrane localisation of wild-type Fzd5-RFP ( wt-fzd5 -RFP; magenta in A,B,G-I; grey in C; arrowheads in C,G,I) and punctate cytoplasmic accumulation of Fzd5 sgu3 -RFP (magenta in D,E,J,K; grey in F; arrowheads in ... More about this image found in The Fzd5 sgu3 protein fails to localise to the cell membrane. (A-K) Cell ...

Fig. 3. Subtle eye defects in fzd5 sgu1 , MZfzd5 sgu1 and fzd5 sgu3 embryos. (A) MZfzd5 sgu1 and fzd5 sgu3 3 days post-fertilisation (dpf) larvae morphology compared to that of wild-type and heterozygote siblings. (B) Quantifications of projected eye area in the genotypic groups fr... More about this image found in Subtle eye defects in fzd5 sgu1 , MZfzd5 sgu1 and fzd5 sgu3 embryos...

Fig. 4. fzd5 sgu1 and fzd5 sgu3 are genetically sensitised to developing eye malformations. (A,B,D-F,H,I) Lateral views, with anterior to the left, of 72 hpf embryos treated with DMSO (A,D,H) or XAV-939 (B,E,F,I). Colobomas (arrows in B,F,I) are evident in a subset of embryos derived from ... More about this image found in fzd5 sgu1 and fzd5 sgu3 are genetically sensitised to developing eye m...