... mutations of the PIK3CD gene that encodes the PI3 kinase delta (PI3Kδ) catalytic subunit. Despite the importance of innate immunity defects in bronchiectasis, there has been limited investigation of neutrophils or macrophages in APDS1 patients or mouse models. Zebrafish embryos provide an ideal system...

...Maaike Allers; Petra A. Bakker; Jelmer Hoeksma; Herman P. Spaink; Jeroen den Hertog ABSTRACT PTPN6 encodes SHP1, a protein tyrosine phosphatase with an essential role in immune cell function. SHP1 mutations are associated with neutrophilic dermatoses and emphysema in humans, which resembles...

... Q61K , HRas G12V and KRas G12V ) in melanocytes, leading to proliferation and changes in morphology of the melanocytes. This model was coupled to binary cell-specific biotagging models allowing in vivo biotinylation and subsequent isolation of macrophage or neutrophil nuclei for regulatory profiling...

...Wenqing Zhou; Lingyan Cao; Jacob Jeffries; Xiaoguang Zhu; Christopher J. Staiger; Qing Deng ABSTRACT Neutrophils are fast-moving cells essential for host immune functions. Although they primarily rely on glycolysis for ATP, isolated primary human neutrophils depend on mitochondrial membrane...

... with the host immune cells (macrophages and neutrophils) to clear Shigella infection in vivo , before being cleared by the zebrafish immune system themselves. Next, an in-depth investigation of Bdellovibrio predation on a range of Gram-negative pathogens using the zebrafish model is needed before...

... composition and degree of immunopathology following low-dose aerosol infection with Mycobacterium tuberculosis . C3HeB/FeJ Tuberculosis Mouse models Chemotherapy Neutrophil At the time of sacrifice, whole lungs were aseptically removed and were used for bacterial enumeration and disrupted...

... new uses for existing drugs – is emerging as an alternative strategy to traditional drug design that promises an improved risk-reward trade-off. Using a zebrafish in vivo neutrophil migration assay, we undertook a drug repositioning screen to identify unknown anti-inflammatory activities for known...

... migration and excess recruitment of neutrophils and other leukocytes can cause damage to the tissue. Here we describe the use of an in vivo model – the Tg(mpx:GFP) i114 zebrafish line, in which neutrophils are labelled by green fluorescent protein (GFP) – to screen a natural product library for compounds...