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Keywords: HDR
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Journal Articles
Journal:
Disease Models & Mechanisms
Dis Model Mech (2023) 16 (2): dmm049874.
Published: 27 February 2023
... technology can effectively disrupt gene function by generating insertions and deletions, but precise gene editing remains challenging. Here, we discuss recent advances in this field. CRISPR/Cas HDR Precise genome editing Base/prime editing Human disease modelling Biotechnology...
Journal Articles
In collection:
Zebrafish as a Disease Model
Annekatrien Boel, Hanna De Saffel, Wouter Steyaert, Bert Callewaert, Anne De Paepe, Paul J. Coucke, Andy Willaert
Journal:
Disease Models & Mechanisms
Dis Model Mech (2018) 11 (10): dmm035352.
Published: 18 October 2018
... repair (HDR) suffers from low efficiency, impeding its use for high-throughput knock-in disease modeling. In this study, we used next-generation sequencing (NGS) analysis to determine the efficiency and reliability of CRISPR/Cas9-mediated HDR using several types of single-stranded oligodeoxynucleotide...
Includes: Supplementary data