...Jens Hansen; Harald von Melchner; Wolfgang Wurst ABSTRACT Gene trapping is a high-throughput approach that has been used to introduce insertional mutations into the genome of mouse embryonic stem (ES) cells. It is performed with generic gene trap vectors that simultaneously mutate and report...

...), and mitochondrial DNA (mtDNA) depletion. A FACS-based retroviral-mediated gene trap mutagenesis screen in mouse embryonic stem (ES) cells for abnormal mitochondrial phenotypes identified a gene trap allele of Sucla2 ( Sucla2 SAβgeo ), which was used to generate transgenic mice. Sucla2 encodes the ADP-specific β...