Summary: A recent study in Nature demonstrated that ongoing mutational processes cause significant heterogeneity of widely used cancer cell lines. In this Editorial, the authors discuss the implications of these findings for the cancer and cell biology fields.
Modeling epigenetic modifications in renal development and disease with organoids and genome editing
Summary: In this Review, we provide an overview on how epigenetic processes are altered in kidney development and disease, and discuss how CRISPR-modified kidney organoids can help us to understand the function of epigenetic marks.
Summary: This Review summarises the current state of the art of single-cell techniques and their potential applications in deciphering the heterogeneous nature of diseases and tailoring personalised therapies.
Summary: Insights into immuno-metabolism open up new avenues for therapeutic intervention. The study presented here suggests that inhibition of glucose suppresses inflammation in a mouse model of ulcerative colitis.
Neuromuscular degeneration and locomotor deficit in a Drosophila model of mucopolysaccharidosis VII is attenuated by treatment with resveratrol
Summary: The authors generate a new Drosophila model of MPS VII, which offers insights into the pathophysiology of this disease, and also uncovers the therapeutic potential of resveratrol in treating MPS VII.
A novel mutation in the NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4 (Ndufa4) gene links mitochondrial dysfunction to the development of diabetes in a rodent model
Summary: Here, we report, for the first time, a major inherited mutation in a diabetes-prone animal model that adversely affects mitochondrial function and leads, through oxidative stress, to the development of diet-induced diabetes.
Pathogenic and non-pathogenic Escherichia coli colonization and host inflammatory response in a defined microbiota mouse model
Summary: Mice harboring a defined microbiota of the altered Schaedler flora were identified as a reliable tool to assess prolonged Escherichia coli intestinal colonization and activation of the host inflammatory response.
Drosophila melanogaster as a function-based high-throughput screening model for antinephrolithiasis agents in kidney stone patients
Summary: Chemical library screens using Drosophila melanogaster as a model of nephrolithiasis can be performed in a high-throughput and efficient manner, leading to candidate drugs with clinical potential in kidney stone patients.
Summary: A novel genetic mouse model of sporadic Beckwith–Wiedemann syndrome (BWS) recapitulates placentomegaly, but placental defects lead to late gestation fetal growth restriction, which contrasts with the fetal overgrowth characteristic of BWS in humans.
Summary: Chronic inflammation collaborates with loss of function of the prostate-specific tumor-suppressor NKX3.1 to promote prostate cancer initiation, increase cellular plasticity and impair cellular differentiation.
Summary: The first Caenorhabditis elegans model to study brain dopamine-serotonin vesicular transport disease, demonstrating impairment of pharyngeal pumping and grazing phenotypes.
Overexpression of Fgfr2c causes craniofacial bone hypoplasia and ameliorates craniosynostosis in the Crouzon mouse
Summary: Increased levels of FGFR2c cause craniofacial bone hypoplasia, microtia and cleft palate, but not craniosynostosis. Introduction of an extra Fgfr2c allele into a mouse model for Crouzon syndrome partially rescues the craniosynostosis phenotype.
Generation of mouse-zebrafish hematopoietic tissue chimeric embryos for hematopoiesis and host-pathogen interaction studies
Editor's choice: A new method to xenotransplant murine bone marrow cells into zebrafish blastulae that generates hundreds of transient chimeric animals with functional murine blood progenitor cells and innate immune cells.
Optimizing the design of population-based patient-derived tumor xenograft studies to better predict clinical response
Summary: A freely available interactive tool is presented that explores the optimal design of a population PDX preclinical study.