There was an error published in Dis. Model. Mech.15, dmm049279 ().
An incorrect version of Fig. 2 was published in which panels G-I had accidentally been deleted. The corrected and original Fig. 2 are shown below. Both the online full-text and PDF versions of the article have been updated with the correct figure.
DMM and the authors apologise for this error and any inconvenience it may have caused.
Nicotinamide riboside (NR) supplementation provides benefits to Tafazzin mutants. (A,B) The abundance of NAD+ (B) and NADH (A) was measured by mass spectrometry and normalized to total protein levels (six biological repetitions with six flies per repetition, data are mean±s.d., two-way ANOVA, genotype effect, P<0.0001, Tukey post-hoc test). (C) The NAD+:NADH ratio was calculated from the abundances for those molecules (data are mean±s.d., two-way ANOVA, genotype effect, P<0.0001, Tukey post-hoc test). (D,E) NR supplementation restores the endurance of Taz889 flies (log-rank analysis, n=8 vials, 20 flies per vial; D) and the respiratory control ratio (RCR) (six biological replicates, data are mean±s.e.m., n=60 per replicate, genotype effect, P<0.0001, NR effect, P=0.0029, Tukey post-hoc test; E). (F) At day 10, the relative mitochondrial DNA (mtDNA) copy number is not different between Taz889 and control flies, but NR supplementation increases mtDNA in Taz889 flies (n=3 biological replicates, data are mean±s.d., two-way ANOVA, genotype effect, P<0.0001, NR effect, P=0.0006, Tukey post-hoc test). (G) Feeding NR to Taz889 flies does not alter the MLCL:CL ratio (unpaired two-tailed Student's t-test, six biological repetitions with six flies per repetition). (H,I) There is no difference in total MLCL (H) between Taz889 flies with and without NR feeding, but NR-fed Taz889 flies have an increased abundance of total CL (I) (unpaired two-tailed Student's t-test, six biological repetitions with six flies per repetition). *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001; ns, not significant.
Nicotinamide riboside (NR) supplementation provides benefits to Tafazzin mutants. (A,B) The abundance of NAD+ (B) and NADH (A) was measured by mass spectrometry and normalized to total protein levels (six biological repetitions with six flies per repetition, data are mean±s.d., two-way ANOVA, genotype effect, P<0.0001, Tukey post-hoc test). (C) The NAD+:NADH ratio was calculated from the abundances for those molecules (data are mean±s.d., two-way ANOVA, genotype effect, P<0.0001, Tukey post-hoc test). (D,E) NR supplementation restores the endurance of Taz889 flies (log-rank analysis, n=8 vials, 20 flies per vial; D) and the respiratory control ratio (RCR) (six biological replicates, data are mean±s.e.m., n=60 per replicate, genotype effect, P<0.0001, NR effect, P=0.0029, Tukey post-hoc test; E). (F) At day 10, the relative mitochondrial DNA (mtDNA) copy number is not different between Taz889 and control flies, but NR supplementation increases mtDNA in Taz889 flies (n=3 biological replicates, data are mean±s.d., two-way ANOVA, genotype effect, P<0.0001, NR effect, P=0.0006, Tukey post-hoc test). (G) Feeding NR to Taz889 flies does not alter the MLCL:CL ratio (unpaired two-tailed Student's t-test, six biological repetitions with six flies per repetition). (H,I) There is no difference in total MLCL (H) between Taz889 flies with and without NR feeding, but NR-fed Taz889 flies have an increased abundance of total CL (I) (unpaired two-tailed Student's t-test, six biological repetitions with six flies per repetition). *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001; ns, not significant.
Nicotinamide riboside (NR) supplementation provides benefits to Tafazzin mutants. (A,B) The abundance of NAD+ (B) and NADH (A) was measured by mass spectrometry and normalized to total protein levels (six biological repetitions with six flies per repetition, data are mean±s.d., two-way ANOVA, genotype effect, P<0.0001, Tukey post-hoc test). (C) The NAD+:NADH ratio was calculated from the abundances for those molecules (data are mean±s.d., two-way ANOVA, genotype effect, P<0.0001, Tukey post-hoc test). (D,E) NR supplementation restores the endurance of Taz889 flies (log-rank analysis, n=8 vials, 20 flies per vial; D) and the respiratory control ratio (RCR) (six biological replicates, data are mean±s.e.m., n=60 per replicate, genotype effect, P<0.0001, NR effect, P=0.0029, Tukey post-hoc test; E). (F) At day 10, the relative mitochondrial DNA (mtDNA) copy number is not different between Taz889 and control flies, but NR supplementation increases mtDNA in Taz889 flies (n=3 biological replicates, data are mean±s.d., two-way ANOVA, genotype effect, P<0.0001, NR effect, P=0.0006, Tukey post-hoc test). (G) Feeding NR to Taz889 flies does not alter the MLCL:CL ratio (unpaired two-tailed Student's t-test, six biological repetitions with six flies per repetition). (H,I) There is no difference in total MLCL (H) between Taz889 flies with and without NR feeding, but NR-fed Taz889 flies have an increased abundance of total CL (I) (unpaired two-tailed Student's t-test, six biological repetitions with six flies per repetition). *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001; ns, not significant.
Nicotinamide riboside (NR) supplementation provides benefits to Tafazzin mutants. (A,B) The abundance of NAD+ (B) and NADH (A) was measured by mass spectrometry and normalized to total protein levels (six biological repetitions with six flies per repetition, data are mean±s.d., two-way ANOVA, genotype effect, P<0.0001, Tukey post-hoc test). (C) The NAD+:NADH ratio was calculated from the abundances for those molecules (data are mean±s.d., two-way ANOVA, genotype effect, P<0.0001, Tukey post-hoc test). (D,E) NR supplementation restores the endurance of Taz889 flies (log-rank analysis, n=8 vials, 20 flies per vial; D) and the respiratory control ratio (RCR) (six biological replicates, data are mean±s.e.m., n=60 per replicate, genotype effect, P<0.0001, NR effect, P=0.0029, Tukey post-hoc test; E). (F) At day 10, the relative mitochondrial DNA (mtDNA) copy number is not different between Taz889 and control flies, but NR supplementation increases mtDNA in Taz889 flies (n=3 biological replicates, data are mean±s.d., two-way ANOVA, genotype effect, P<0.0001, NR effect, P=0.0006, Tukey post-hoc test). (G) Feeding NR to Taz889 flies does not alter the MLCL:CL ratio (unpaired two-tailed Student's t-test, six biological repetitions with six flies per repetition). (H,I) There is no difference in total MLCL (H) between Taz889 flies with and without NR feeding, but NR-fed Taz889 flies have an increased abundance of total CL (I) (unpaired two-tailed Student's t-test, six biological repetitions with six flies per repetition). *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001; ns, not significant.
