..., we showed that wild-type Zar1, but not Zar1-mu, downregulated ZP gene translation ( Fig. 8 E-G). Fig. 7. Translational upregulation of zona pellucida proteins may cause ER stress. (A) ZP proteins are upregulated in zar1 homozygous (homo) ovaries compared with heterozygous (hetero) ovaries...

... adhesion to the rigid extracellular matrix of the oocyte, the zona pellucida, which was released coincident with GLYT1 activation. The ability to acutely regulate cell size is thus acquired by the oocyte only after ovulation, when it first develops glycine-dependent cell volume regulation. * Author...

...Carey Rodeheffer; Barry D. Shur During mammalian fertilization, sperm adhere to the extracellular coat of the egg, or zona pellucida, in a species-specific manner. In mouse, evidence suggests that sperm recognize and bind to specific oligosaccharide ligands within the zona pellucida glycoprotein...

...Tracy L. Rankin; Marilyn O’Brien; Eric Lee; Karen Wigglesworth; John Eppig; Jurrien Dean ABSTRACT All vertebrate eggs are surrounded by an extracellular matrix. This matrix is known as the zona pellucida in mammals and is critically important for the survival of growing oocytes, successful...

... by the onset of cleavage. Author for correspondence (e-mail: [email protected] ) 26 February 2001 8 January 2001 © 2001 by Company of Biologists 2001 Blastocyst Embryonic-abembryonic axis Bilateral axis Animal-vegetal axis Zygote Alginate gel Zona pellucida...

...Xudong Shi; Simin Amindari; Kavitha Paruchuru; Dale Skalla; Heather Burkin; Barry D. Shur; David J. Miller ABSTRACT ZP3 is a protein in the mammalian egg coat (zona pellucida) that binds sperm and stimulates acrosomal exocytosis, enabling sperm to penetrate the zona pellucida. The nature of the ZP3...

...Tracy Rankin; Prue Talbot; Eric Lee; Jurrien Dean All vertebrates have an egg shell that surrounds ovulated eggs and plays critical roles in gamete recognition. This extracellular matrix is known as the zona pellucida in eutherian mammals and consists of three glycoproteins, ZP1, ZP2 and ZP3...

...Tracy L. Rankin; Zhi-Bin Tong; Philip E. Castle; Eric Lee; Robert Gore-Langton; Lawrence M. Nelson; Jurrien Dean ABSTRACT The mammalian zona pellucida surrounding ovulated eggs mediates sperm binding at fertilization, provides a postfertilization block to polyspermy, and facilitates passage of pre...

...Li-fang Liang; Selma M. Soyal; Jurrien Dean ABSTRACT The mouse zona pellucida is composed of three glycoproteins, ZP1, ZP2 and ZP3, encoded by single-copy genes whose expression is temporally and spatially restricted to oocytes. All three proteins are required for the formation of the extracellular...

... and are independent of the state of intracel-lular galactosylation during spermatogenesis. * Author for correspondence (email: [email protected] ) 07 08 1997 © 1997 by Company of Biologists 1997 galactosyltransferase fertilization sperm zona pellucida mouse ZP3 Species...

...Tracy Rankin; Mary Familari; Eric Lee; Ann Ginsberg; Nancy Dwyer; Joan Blanchette-Mackie; John Drago; Heiner Westphal; Jurrien Dean ABSTRACT Mammalian oocytes synthesize and secrete a zona pellucida that surrounds the growing oocytes, ovulated eggs and preimplantation embryos. The extracellular...

...Olga Epifano; Li-fang Liang; Mary Familari; Malcolm C. Moos, Jr; Jurrien Dean ABSTRACT The mammalian zona pellucida is an extracellular matrix that surrounds growing oocytes, ovulated eggs and early embryos. The mouse zona is composed of three sulfated glycoproteins: ZP1, ZP2 and ZP3. Each...

... , 388 – 389 . Bleil , J.D. , Beall , C.F. and Wassarman , P.M. ( 1981 ). Mammalian sperm-egg interaction: Fertilization of mouse eggs triggers modifications of the major zona pellucida glycoprotein, ZP2 . Dev. Biol . 86 , 189 – 197 . Buck , W.R. , Hoffman , E.E...

... manner the ZP2 to ZP2 f conversion that is involved in the zona pellucida block to polyspermy, as well as the ZP2 to ZP2 f conversion promoted by microinjected IP 3 in non-inseminated eggs. As anticipated, inseminated eggs that had been microinjected with the antibody were polyspermic. In addition...

...David J. Miller; Xiaohai Gong; Barry D. Shur ABSTRACT Fertilization in the mouse is initiated by sperm 1,4-galactosyltransferase (GalTase) binding to terminal N -acetylglucosamine residues on the zona pellucida glyco-protein ZP3. Binding of ZP3 induces exocytosis of the sperm acrosome, whose...

...Ross A. Kinloch; Steven Mortillo; Paul M. Wassarman ABSTRACT Sperm receptors are located in the mammalian egg extracellular coat, or zona pellucida. Mouse and hamster sperm receptor glycoproteins, mZP3 (83 × 10 3 M r ) and hZP3 (56 × 10 3 M r ), respectively, have very similar polypeptides (44×10 3...

... exclusively in the extracellular coat (zona pellucida) of mammalian eggs. ( If ) ZP3 gene expression is an example of oocyte-specific and, therefore, sex-specific gene expression during mammalian development. ( Ill ) ZP3 is a structural glycoprotein Involved in assembly of the egg extracellular coat during...

... was studied firstly by determining whether any of the secreted proteins bound to the zona pellucida. The results of iodination studies showed that two polypeptides of M T 92 and 46×10 3 , respectively, were bound to the zona pellucida of eggs removed from the oviduct but were absent from eggs that had not had...

...C. R. Brown; R. Jones ABSTRACT Experiments have been carried out to identify proteins on boar spermatozoa that bind to components of the zona pellucida. Polypeptides in sodium deoxycholate extracts of boar spermatozoa and in whole seminal plasma have been separated by sodium dodecyl sulphate...

...C. R. Brown; W. K. T. Cheng ABSTRACT Our objective was to identify any changes that occur in the composition of the porcine zona pellucida during development of the 2-to 4-cell embryo from the oocyte. Oocytes, unfertilized eggs and single and 2-to 4-cell embryos have been recovered surgically...