... resolution in space and time can help reveal how embryonic cells decode these signals to make appropriate fate decisions. Here, we present new optogenetic reagents and an experimental pipeline for creating designer Nodal signaling patterns in live zebrafish embryos. Nodal receptors were fused to the light...

... molecular regulation of C&E differs between tissues remains an open question. Using a zebrafish explant model of anteroposterior axis extension, we show that C&E of the neuroectoderm and mesoderm can be uncoupled ex vivo , and that morphogenesis of individual tissues results from distinct morphogen...

... as they colonized peripheral tissues during zebrafish development to determine whether cells tailor their migration strategy to their local tissue environment. We found that T cells in most sites migrated with F-actin-rich, leading-edge pseudopods, matching how they migrate in vitro . T cells notably deviated from...

... linking receptor activation to gene regulation remain poorly understood. As a first step towards elucidating this mechanism, we examined the function of the Gq/11 family of Gα subunits in zebrafish using pharmacological inhibition and genetic ablation of Gq/11 activity, and transgenic induction...

...Wenyi Yang; Mingjian Peng; Youquan Wang; Xiaowen Zhang; Wei Li; Xue Zhai; Zhichao Wu; Peng Hu; Liangbiao Chen ABSTRACT Iron is essential for cell growth and hematopoiesis, which is regulated by hepcidin ( hamp ). However, the role of hamp in zebrafish hematopoiesis remains unclear. Here, we have...

... generate zebrafish transgenic lines with continuous fluorescent labelling specific to the endoderm, and demonstrate its effectiveness in long-term tracing by showing the progression of endoderm development from embryo to adult, providing visualisation of endodermal cells and their derived tissues...

...Kun Jia; Bo Cheng; Lirong Huang; Jiaxin Xu; Fasheng Liu; Xinjun Liao; Kai Liao; Huiqiang Lu ABSTRACT Biliary epithelial cell (BEC)-derived liver regeneration in zebrafish exhibits similarities to liver regeneration in chronic liver injury. However, the underlying mechanisms remain poorly understood...

...Anupama Rao; Andrew Russell; Jose Segura-Bermudez; Charles Franz; Rejenae Dockery; Anton Blatnik; Jacob Panten; Mateo Zevallos; Carson McNulty; Maciej Pietrzak; Joseph Aaron Goldman ABSTRACT Zebrafish have a high capacity to regenerate their hearts. Several studies have surveyed transcriptional...

...). Using a temporal-spatial resolved fate-mapping system, here we show that, in zebrafish, DCs arise from two sources: dorsal aorta-born endothelium-derived hematopoietic progenitors (EHPs) and HSCs. The EHP-derived DCs emerge early, predominantly colonizing the developing thymus during larval stages...

... attributable to failed differentiation or loss of non-skeletal neural crest derivatives. We report here an additional, previously undescribed requirement for Sox10 in bone mineralization. Neither crest- nor mesoderm-derived bones initiate mineralization on time in zebrafish sox10 mutants, despite normal...

... removal of dorsal progenitors in the zebrafish tailbud using multiphoton ablation. This led to a proportional reduction in the length of the spinal cord and paraxial mesoderm in the tail, revealing a capacity for the regulation of tissue morphogenesis during tail formation. Following analysis of cell...

...Yusuke Mori; Sierra Smith; Jiacheng Wang; Nadia Eliora; Kira L. Heikes; Akankshi Munjal ABSTRACT During inner ear semicircular canal morphogenesis in zebrafish, patterned canal-genesis zones express genes for extracellular matrix component synthesis. These include hyaluronan and the hyaluronan...

...Shengnan Liu; Zhi Feng; Ming Su; Chenchen Liu; Yuan Xi; Huan Chen; Lingfei Luo; Xin Tian; Fangying Zhao; Li Li ABSTRACT Haematopoietic stem and progenitor cells (HSPCs) arise from the aorta-gonad-mesonephros and migrate to the caudal haematopoietic tissue (CHT) in zebrafish, where nascent HSPCs...

... contribute to neurons during the first neurogenic phase, whereas boundary cells participate later. To analyze what maintains boundary cells as non-neurogenic progenitors, we addressed the role of Her9, a zebrafish Hes1-related protein. her9 expression is temporarily sustained in boundary cells independently...

... progenitors to induce the switch from neurogenesis to oligodendrogenesis in the zebrafish hindbrain. Fgf20 emanating from earlier-born neurons signals at a short range to downregulate proneural gene expression in the segment centre with high spatial precision along both anterior-posterior and dorsal-ventral...

... appendages, such as the dorsal and anal fins. Compared with paired fins, and limbs, median appendages remain surprisingly understudied. Here, we report that a dominant zebrafish mutant, smoothback ( smb ), fails to develop a dorsal fin. Moreover, the anal fin is reduced along the antero-posterior axis...

... in vitro , their functions in vivo remain unclear. Using a chemical-genetic screen in zebrafish, we found that GPRC6A, a G-protein-coupled receptor, mediates non-genomic androgen actions during embryonic development. Exposure to androgens (androstanedione, DHT and testosterone) caused cardiac edema or tail...

... heart failure for several years, but with little success. Here, we investigate a different approach to augment VEGFA bioavailability: by deleting the VEGFA decoy receptor VEGFR1 (also known as FLT1), one can achieve more physiological VEGFA concentrations. We find that after cryoinjury, zebrafish flt1...

...Frédéric Rosa; Nicolas Dray; Sébastien Bedu; Laure Bally-Cuif ABSTRACT Neural stem cells (NSCs) generate neurons over a lifetime in adult vertebrate brains. In the adult zebrafish pallium, NSCs persist long term through balanced fate decisions. These decisions include direct neuronal conversions...

... sources and specific functions in heart development remain unclear. To address this, we created a vegfc reporter and an inducible overexpression line in zebrafish. We found vegfc expression in large coronary arteries, circulating thrombocytes, cardiac adipocytes, and outflow tract smooth muscle cells...