... that the interplay of Crb expression in the DV boundary and ubiquitin-dependent Crb downregulation by Ebi provides a mechanism for the maintenance of Notch signaling during wing development. * Present address: Department of Developmental and Regenerative Biology and Graduate School of Biomedical Sciences...

..., controlling both the density and timing of lateral root emergence in Arabidopsis. Arabidopsis Phytohormone Saccharomyces Ubiquitin The plant hormone auxin directs many developmental responses, including the elaboration of branching patterns in the root. Among the first steps in auxin...

...Chenggang Lu; Jongmin Kim; Margaret T. Fuller The ubiquitin proteasome system (UPS) regulates many biological pathways by post-translationally ubiquitylating proteins for degradation. Although maintaining a dynamic balance between free ubiquitin and ubiquitylated proteins is key to UPS function...

... of the proteasome in lens fiber differentiation. We found that an E3 ubiquitin ligase anaphase-promoting complex/cyclosome (APC/C) is involved in lens defects in the vov mutant. These data suggest that the ubiquitin proteasome system is required for cell proliferation of lens epithelium and for the differentiation...

.... Nutcracker interacts through its F-box domain with members of a Cullin-1-based ubiquitin ligase complex (SCF): Cullin-1 and SkpA. This ubiquitin ligase does not regulate the stability of the caspase inhibitors DIAP1 and DIAP2, but physically binds Bruce, a BIR-containing giant protein involved in apoptosis...

...Katia Marrocco; Alexis Thomann; Yves Parmentier; Pascal Genschik; Marie Claire Criqui Selective protein degradation via the ubiquitin-26S proteasome is a major mechanism underlying DNA replication and cell division in all eukaryotes. In particular, the APC/C (anaphase promoting complex or cyclosome...

... of the neural crest regulatory program. We identify Partner of paired (Ppa) as the F-box component of a modular E3 ligase, and show that it is expressed in neural crest-forming regions, and that it binds to and promotes ubiquitin-mediated proteasomal degradation of Slug. Misexpression of Ppa inhibits...

... of the neural crest lineage during embryogenesis. We describe the identification of Xenopus Cullin-1 , an E3 ubiquitin ligase, and show that blocking the function of endogenous Cullin-1 leads to pleiotropic defects in development. Notably, there is an increased allocation of cells to a neural crest fate...

... is necessary for three different Notch/Delta signaling events at the morphogenetic furrow, Faf is essential only for one:Delta signaling by photoreceptor precluster cells, which prevents recruitment of ectopic neurons. In addition, we show that the ubiquitin-ligase Neuralized(Neur), which ubiquitinates Delta...

... to isolate lin-35 synthetic-lethal mutations, we have identified redundant roles for lin-35 and ubc-18 , a gene that encodes an E2 ubiquitin-conjugating enzyme closely related to human UBCH7. lin-35 and ubc-18 cooperate to control one or more steps during pharyngeal morphogenesis. Based on genetic...

... encodes a HECT domain E3 ubiquitin ligase that is likely to act by targeting Cubitus interruptus and an unknown activator of hedgehog expression for proteolysis. References Alcedo, J., Ayzenzon, M., von Ohlen, T., Noll, M. and Hooper, J. E. (1996). The Drosophila smoothened gene encodes a seven...

...Sergey Doronkin; Inna Djagaeva; Steven K. Beckendorf The COP9 signalosome (CSN) is linked to signaling pathways and ubiquitin-dependent protein degradation in yeast, plant and mammalian cells,but its roles in Drosophila development are just beginning to be understood. We show that during oogenesis...

... E3 ligases, implying a key role in ubiquitin-proteasome mediated protein degradation. We report that Arabidopsis FUS11 gene encodes the subunit 3 of the COP9 signalosome (CSN3). The fus11 mutant is defective in the COP9 signalosome and accumulates significant amount of multi-ubiquitinated proteins...

...Angelica L. M. Cadavid; Andrew Ginzel; Janice A. Fischer Fat facets is a deubiquitinating enzyme required in a cell communication pathway that limits to eight the number of photoreceptor cells in each facet of the Drososphila compound eye. Genetic data support a model whereby Faf removes ubiquitin...

... an inhibitory signal to extraneous cells within the facet preclusters. Generation of the inhibitory signal requires the ubiquitin-specific protease encoded by the fat facets gene and is thus regulated by ubiquitin-dependent proteolysis. * Author for correspondence (e-mail: [email protected]...

...Patrick H. Krone; John J. Heikkila Heat-induced accumulation of hsp 30 mRNA (1·1 kb) during early development of Xenopus laevis was first detectable at the tailbud stage (stage 30–34). This contrasts with heat-induced accumulation of hsp 70 mRNA (2·7 kb) and ubiquitin mRNA (size range = 1·7–3·1 kb...