... of microscopic size. Here, we describe a simple protocol to generate macroscopic intestinal tubes from small cystic organoids. Embedding proliferating organoids within a contracting floating collagen gel allows them to align and fuse to generate macroscopic hollow structures (‘tubes’) that are lined...

[email protected] ) Competing interests statement The authors declare no competing financial interests. 11 10 2011 © 2011. Drosophila Rho GTPase Actin Lumen Salivary gland Tube Epithelial tubes are the structural and functional components of many essential...

...Baohe Shen; James L. Manley ABSTRACT Determination of dorsal/ventral polarity in Drosophila requires 12 genetically defined, maternally encoded proteins. These include Toll, a transmembrane receptor, Pelle, a ser/thr protein kinase and Tube, all of which function intracytoplasmically to initiate...

...David N. Edwards; Par Towb; Steven A. Wasserman ABSTRACT A signaling pathway active on the ventral side of the Drosophila embryo defines dorsoventral polarity. A cell surface signal relayed by Toll, Tube and Pelle releases the Rel-related protein Dorsal from its cytoplasmic inhibitor Cactus; free...

...Rene L. Galindo; David N. Edwards; Susan K. H. Gillespie; Steven A. Wasserman ABSTRACT Within the Drosophila embryo, tube and the protein kinase pelle transduce an intracellular signal generated by the transmembrane receptor Toll. This signal directs import of the rel-related protein dorsal...