...Anish Dattani; Tao Huang; Corin Liddle; Austin Smith; Ge Guo ABSTRACT Propagation of human naïve pluripotent stem cells (nPSCs) relies on the inhibition of MEK/ERK signalling. However, MEK/ERK inhibition also promotes differentiation into trophectoderm (TE). Therefore, robust self-renewal requires...

... for trophectodermal Tat-Cre/loxP recombination. We used the natural permeability barrier in mouse blastocysts in combination with off-the-shelf Tat-Cre recombinase to achieve editing of conditional alleles in the trophoblast lineage. This direct approach enables gene function analysis during implantation...

... NANOG expression in human epiblast cells, consistent with a requirement for this pathway in pluripotency. Although the key trophectoderm factors Id2 , Elf5 and Eomes are exclusively localized to this lineage in the mouse, the human orthologues are either absent or expressed in alternative lineages...

... how the chromatin remodelling protein CHD4 facilitates the first lineage decision in mammalian embryogenesis. Embryos lacking CHD4 can form a morphologically normal early blastocyst, but are unable to successfully complete the first lineage decision and form functional trophectoderm (TE...

...Tomoko Watanabe; John S. Biggins; Neeta Bala Tannan; Shankar Srinivas The formation of trophectoderm (TE) and pluripotent inner cell mass (ICM) is one of the earliest events during mammalian embryogenesis. It is believed that the orientation of division of polarised blastomeres in the 8- and 16...

... outer trophectoderm (TE) and internal primitive endoderm (PE) in the blastocyst and subsequently give rise to chorio-allantoic and visceral yolk sac placentae, respectively. We have shown maternal low protein diet exclusively during mouse preimplantation development (Emb-LPD) is sufficient to cause...

...Kotaro J. Kaneko; Melvin L. DePamphilis It has been suggested that during mouse preimplantation development, the zygotically expressed transcription factor TEAD4 is essential for specification of the trophectoderm lineage required for producing a blastocyst. Here we show that blastocysts can form...

... choice . Reprod. Biomed. Online 22 , 521 - 524 . Ralston A. , Rossant J. ( 2008 ). Cdx2 acts downstream of cell polarization to cell-autonomously promote trophectoderm fate in the early mouse embryo . Dev. Biol. 313 , 614 - 629 . Ralston A. , Cox B. J...

... and the trophectoderm. Shortly after implantation, the anterior-posterior axis is firmly established. Recent studies have provided a better understanding of how the earliest cell fate decisions are regulated in the pre-implantation embryo, and how and when the body axes are established in the pregastrulation embryo...

...Guangming Wu; Luca Gentile; Takuya Fuchikami; Julien Sutter; Katherina Psathaki; Telma C. Esteves; Marcos J. Araúzo-Bravo; Claudia Ortmeier; Gaby Verberk; Kuniya Abe; Hans R. Schöler The separation of the first two lineages – trophectoderm (TE) and inner cell mass (ICM) – is a crucial event...

...-implantation embryo. Here, we show that Klf5 is required for the formation of the trophectoderm (TE) and the inner cell mass (ICM), and for repressing primitive endoderm (PE) development. Although cell polarity appeared normal, Klf5 mutant embryos arrested at the blastocyst stage and failed to hatch due...

...Robert Odell Stephenson; Yojiro Yamanaka; Janet Rossant The first two cell lineages in the mouse, the surface trophectoderm (TE) and inner cell mass (ICM), are morphologically distinguishable by E3.5, with the outer TE forming a polarized epithelial layer enclosing the apolar ICM. We show here...

... in the trophectoderm of peri-implantation embryo and directly regulates Cdx2 gene expression . J. Biol. Chem. 284 , 28729 - 28737 . Kehler J. , Tolkunova E. , Koschorz B. , Pesce M. , Gentile L. , Boiani M. , Lomeli H. , Nagy A. , McLaughlin K. J. , Scholer H...

... to facilitate or to suppress cell-cycle progression. In stark contrast to the milder phenotypes that result from inactivation of E2Fs, we report that loss of Dp1 leads to death in utero because of the failure of extra-embryonic development. Loss of Dp1 compromises the trophectoderm-derived tissues...

...Bhavwanti Sheth; Breda Moran; James M. Anderson; Tom P. Fleming ABSTRACT The mouse blastocyst forms during the 32-cell stage with the emergence of the blastocoelic cavity. This developmental transition is dependent upon the differentiation and transport function of the trophectoderm epithelium...

...Bhavwanti Sheth; Ira Fesenko; Jane E. Collins; Breda Moran; Arthur E. Wild; James M. Anderson; Tom P. Fleming ABSTRACT The mouse preimplantation embryo has been used to investigate the de novo synthesis of tight junctions during trophectoderm epithelial differentiation. We have shown previously...

.... Previously, we have shown that desmocollin protein synthesis is undetectable in eggs and cleavage stages but initiates at the early blastocyst stage when desmocollin localises at, and appears to regulate assembly of, nascent desmosomes that form in the trophectoderm but not in the inner cell mass (Fleming, T...

... in the absence of extracellular calcium. Cingulin synthesis is also tissue-specific in blastocysts, being up-regulated in trophectoderm and down-regulated in the inner cell mass. Taken together, the results suggest that (i) cingulin may have a role during oogenesis and (ii) cell-cell contact patterns regulate...

...Tom P. Fleming; Qamar Javed; Mark Hay ABSTRACT Trophectoderm differentiation during blastocyst formation provides a model for investigating how an epithelium develops in vivo. This paper briefly reviews our current understanding of the stages of differentiation and possible control mechanisms...

..., desmoplakins), dp3 (plakoglobin), desmosomal glycoprotein 1 (dgl, desmoglein) and dg2+3 (desmocollins). Immunofluorescence labelling of staged intact embryos and synchronised cell clusters indicates that dp1+2, dgl and dg2+3 are first detectable on the lateral membrane contact sites between trophectoderm cells...