... that encode direct mediators of circuit remodeling within developing cells. To identify potential drivers of sensory-dependent synaptic development, we generated a single-nucleus RNA sequencing dataset describing the transcriptional responses of cells in the mouse visual cortex to sensory deprivation...

... properties in nc14 like decreasing the duration of a long transcriptionally inactive (OFF) state (long inter-burst periods). Our analysis further reveals changes of these transcriptional bursting properties across space, suggesting a mispatterning at the kinetic level. This analysis was only possible through...

... imaging tools allow imaging nascent transcription in various tissues, keeping tissues alive and transcriptionally active during live imaging is an important consideration. However, many protocols already exist that allow ex vivo imaging of tissues such as we have used here in the larval discs ( Dye...

... response is mostly mediated by repression of transcriptional targets that occur in tandem with reductions in chromatin accessibility at regulatory regions. Furthermore, immediate early ERK responses are largely shared by different cell types but produce cell-specific programmes as these responses interface...

... and tuning of its genomic plasticity. Foxg1 represses gene transcription, and L1 elements share putative Foxg1-binding motifs, suggesting the former might limit telencephalic expression (and activity) of the latter. We tested such a prediction, in vivo as well as in engineered primary neural cultures, using...

... HDAC1-3, NCoR (NCOR1) and SMRT (NCOR2) ( Gottlieb et al., 2002 ; Sims et al., 2002 ). Second, SMYD1 functions as a transcriptional activator by catalyzing trimethylation of H3K4, a histone modification associated with transcriptionally active loci ( Tan et al., 2006 ). Lastly, in skeletal and heart...

...Michael F. Rogers; Owen J. Marshall; Julie Secombe ABSTRACT Histone-modifying proteins play important roles in the precise regulation of the transcriptional programs that coordinate development. KDM5 family proteins interact with chromatin through demethylation of H3K4me3 as well as demethylase...

...Min Tang; Isabel Regadas; Sergey Belikov; Olga Shilkova; Lei Xu; Erik Wernersson; Xuewen Liu; Hongmei Wu; Magda Bienko; Mattias Mannervik ABSTRACT The histone deacetylase HDAC3 is associated with the NCoR/SMRT co-repressor complex, and its canonical function is in transcriptional repression...

... to activate MYC transcription. Psi, the sole FUBP protein in Drosophila , binds RNA to regulate P-element and mRNA splicing. Our previous work revealed pro-growth functions for Psi, which depend, in part, on transcriptional activation of Myc. Genome-wide functions for FUBP family proteins in transcriptional...

...Gianluca Zambanini; Anna Nordin; Mattias Jonasson; Pierfrancesco Pagella; Claudio Cantù ABSTRACT Upon WNT/β-catenin pathway activation, stabilized β-catenin travels to the nucleus where it associates with the TCF/LEF transcription factors, constitutively bound to genomic Wnt-responsive elements...

.... A strong translational selection of low-abundance transcripts involved in metabolic pathways and lysosomes was found throughout bovine embryonic development. Notably, genes involved in mitochondrial function were prioritized for translation. We found that translation largely reflected transcription...

...Laura J. Harrison; Daniel Bose ABSTRACT Enhancers confer precise spatiotemporal patterns of gene expression in response to developmental and environmental stimuli. Over the last decade, the transcription of enhancer RNAs (eRNAs) – nascent RNAs transcribed from active enhancers – has emerged...

...Cai-Li Bi; Qian Cheng; Ling-Yue Yan; Hong-Yan Wu; Qiang Wang; Ping Wang; Lin Cheng; Rui Wang; Lin Yang; Jian Li; Feng Tie; Hao Xie; Ming Fang ABSTRACT The evolutionarily conserved C-terminal binding protein (CtBP) has been well characterized as a transcriptional co-repressor. Herein, we report...

... establishment of female gametes is orchestrated through complex oocyte transcription networks that must also properly distinguish germ cell and somatic cell lineages. In addition to the more well-known enhancer-bound transcription activators and repressors, tissue-selective components of the basal transcription...

.... First, activation of target-gene expression expands over time from the ventral-most region of high nuclear Dorsal to lateral regions, where the levels are lower, as a result of a Dorsal-dependent activation probability of transcription sites. Thus, sites that are activated earlier will exhibit more mRNA...

... underlying gene regulation. This Review covers core technologies, concepts, recent advances and applications of in vivo single-molecule tracking. Fluorescence Gene regulation Light-sheet Single-molecule microscopy Single-molecule tracking Transcription Children's Hospital...

...Margherita Peron; Alberto Dinarello; Giacomo Meneghetti; Laura Martorano; Riccardo M. Betto; Nicola Facchinello; Annachiara Tesoriere; Natascia Tiso; Graziano Martello; Francesco Argenton ABSTRACT The STAT3 transcription factor, acting both in the nucleus and mitochondria, maintains embryonic stem...

... by pioneering studies of Drosophila neuroblasts. In these lineages, the transcription factors hunchback (Hb), krüppel (Kr), Nub/Pdm2 (collectively called Pdm) and castor (Cas) are part of a temporal identity cascade where each factor is necessary and sufficient for the generation of neurons born during...

...Brett R. Lancaster; James D. McGhee ABSTRACT We define a quantitative relationship between the affinity with which the intestine-specific GATA factor ELT-2 binds to cis -acting regulatory motifs and the resulting transcription of asp-1 , a target gene representative of genes involved...

... silencing complex (RISC). The mechanism for growth inhibition does not, however, involve canonical roles as part of the RISC; rather, AGO1 controls cell and tissue growth by functioning as a direct transcriptional repressor of the master regulator of growth, Myc. AGO1 depletion in wing imaginal discs drives...