...Stéphane Zaffran; Ingolf Reim; Li Qian; Patrick C. Lo; Rolf Bodmer; Manfred Frasch The NK homeobox gene tinman ( tin ) is required for the specification of the cardiac, visceral muscle and somatic muscle progenitors in the early dorsal mesoderm of Drosophila. Like its vertebrate counterpart Nkx2.5...

... remain unknown. Here, we show that the highly conserved basic helix-loop-helix (bHLH)transcription factor Hand is expressed in cardioblasts, pericardial nephrocytes and hematopoietic progenitors. The homeodomain protein Tinman and the GATA factors Pannier and Serpent directly activate Hand in these cell...

... of Tinman, a mesoderm-specific homeobox transcription factor. We present evidence that the Gata transcription factor, Pannier, and its binding partner U-shaped, also a zinc-finger protein, cooperate in the process of heart development. Loss-of-function and germ layer-specific rescue experiments suggest...

.... References Alcedo, J. and Noll, M. ( 1997 ). Hedgehog and its Patched-Smoothened receptor complex: a novel signaling mechanism at the cell surface. Biol. Chem. 378 , 583 -590. Azpiazu, N. and Frasch, M. ( 1993 ). tinman and bagpipe: Two homeobox genes that determine cell fates in the dorsal...

... and Anatomy, University of Arizona College of Medicine, 1601 N. Campbell Avenue, Tucson, AZ 85724, USA (e-mail: [email protected] ) 08 10 1999 08 12 1999 © 1999 by Company of Biologists 1999 Left/right axis Spleen Nkx2-5 Homeobox gene tinman Polysplenia Vertebrate...

... repressing a pericardial cell fate. Ectopic expression of Pannier results in cardial cell overproduction, while co-expression of Pannier and the homeodomain protein Tinman synergistically activate cardiac gene expression and induce cardial cells. The related GATA4 protein of mice likewise functions...

... homeobox gene tinman (tin) is expressed in cardiac and visceral mesodermal progenitors and is essential for their specification. In vertebrates, the tin homologue Nkx2-5/Csx and related genes are expressed in early cardiac and visceral mesodermal progenitors. To test for an early cardiogenic function...

... into the characteristic ‘ladder’ structure found at this stage in wild-type embryos ( Fig. 4D ). Gonadal mesoderm Fat body Drosophila abdominal A Abdominal B clift serpent tinman zinc finger homeodomain protein-1 Developmental programs leading to the specification and differentiation...

...Zhizhang Yin; Xiao-Lei Xu; Manfred Frasch ABSTRACT The Drosophila tinman homeobox gene has a major role in early mesoderm patterning and determines the formation of visceral mesoderm, heart progenitors, specific somatic muscle precursors and glia-like mesodermal cells. These functions of tinman...

... and pericardial cell precursors and is critically dependent on mesodermal tinman function, epidermal Wingless signaling and the coordinate action of neurogenic genes. Negative regulation by hedgehog is required to restrict ladybird expression to two out of six cardioblasts in each hemisegment. Overexpression...

... in the adult heart, with only trace levels in skeletal muscle. During murine embryogenesis, endogenous carp expression was first clearly detected as early as E8.5 specifically in heart and is regulated temporally and spatially in the myocardium. Nkx2-5 , the murine homologue of Drosophila gene tinman...

... process leading to heart muscle and those that define the earliest embryonic heart field, but for neither class are genes defined. We cloned zebrafish Nkx2 . 5 , a homolog of the tinman homeodomain gene needed for visceral and cardiac mesoderm formation in Drosophila . In the zebrafish, its expression...

...Ondine B. Cleaver; Kristin D. Patterson; Paul A. Krieg ABSTRACT Drosophila tinman is an NK-class homeobox gene required for formation of the dorsal vessel, the insect equivalent of the vertebrate heart. Vertebrate sequences related to tinman , such as mouse Nkx-2 . 5 , chicken cNkx-2 . 5, Xenopus...

...Sylvia M. Evans; Wei Yan; M. Patricia Murillo; Jeanette Ponce; Nancy Papalopulu ABSTRACT tinman is a Drosophila Nk-homeobox gene required for heart and visceral mesoderm specification. Mutations in tinman result in lack of formation of the Drosophila heart, the dorsal vessel. We have isolated an Nk...

...Rolf Bodmer ABSTRACT The homeobox-containing gene tinman (msh-2 , Bodmer et al., 1990 Development 110, 661-669) is expressed in the mesoderm primordium, and this expression requires the function of the mesoderm determinant twist . Later in development, as the first mesodermal subdivisions...