...://www.biologists.com/user-licence-1-1/ Summary: Using body axis elongation in Drosophila embryos as a model, novel image analysis and machine learning methods identify cell divisions oriented by mechanical cues to reduce strain. Image analysis Machine learning Time-lapse microscopy Morphogenesis...

... and blood flow in avian embryos allows precise quantification of the hemodynamic parameters that regulate vascular remodelling. Angiogenesis Arteriogenesis Hemodynamics Time-lapse microscopy Micro-particle image velocimetry Computational fluid dynamics Vascular remodelling is an essential...

... is understudied. We recently developed a technique to simultaneously analyse flow dynamics and vascular remodelling by time-lapse microscopy in the capillary plexus of avian embryos and used this to study the hemodynamic environment present during angiogenic sprouting. We found that sprouts always form from...

... and nondividing cells. When compared with nondividing cells, dividing cells showed a reduction in the ratio of apical to basolateral TdTomato, consistent with them losing a degree of apicobasolateral polarity during division ( Fig. 2 C-E). Fig. 1. 4D time-lapse microscopy of blastocyst formation...

... for Biotechnology CNB-CSIC, E-28049 Madrid, Spain. Fgf signaling Wnt signaling Embryonic elongation Segmentation clock Signal gradient Time-lapse microscopy Surgical manipulations in chick show that segments form rhythmically and sequentially even when the PSM is separated from...

... cell Time-lapse microscopy Cell tracking Mouse Cell cycle progression and lineage commitment of stem cells appear to be tightly linked processes ( Lange and Calegari, 2010 ; Orford and Scadden, 2008 ; Singh and Dalton, 2009 ). In particular, the early G1 phase of the cell cycle...

...-lapse microscopy Mounting Light sheet microscopy SPIM Fluorescence microscopy is an invaluable tool for in vivo studies of developmental processes. However, excessive light exposure in commonly employed techniques, such as confocal, two-photon or spinning disc systems, causes...

.... * Author for correspondence ( [email protected] ) Competing interests statement The authors declare no competing financial interests. 18 4 2011 © 2011. 2011 Hemangioblast Hemogenic endothelium Serum-free differentiation Single cell colonial differentiation Time-lapse microscopy...

... 2009. 2009 Hirschsprung's disease Endothelin receptor B Multicellular invasion Neural crest cells Time-lapse microscopy Tissue graft Cre-lox Colon Mouse The enteric nervous system (ENS) is formed primarily from neural crest cells (NCCs) from the post-otic hindbrain (vagal NCCs...

... signalling pathways can generate cyclopic phenotypes by disrupting normal cell movements or introducing new erroneous behaviours. * Author for correspondence (e-mail: [email protected] ) 4 10 2006 © 2006. 2006 Zebrafish Time-lapse microscopy Neurulation Cyclopia Quantitative...

.... and Kane , D. A. ( 1990 ). Cell-autonomous action of zebrafish spt-1 mutation in specific mesodermal precursors . Nature 348 , 728 – 30 . 10.1038/348728a0 Kane , D. A. and Warga , R. M. ( 1991 ). Zebrafish development . In A Dozen Eggs: Time-lapse Microscopy of Normal...

... the cellular changes and dynamics of furrow formation, we examined living Drosophila embryos using three-dimensional time-lapse microscopy. By injecting fluorescent markers that visualize cell outlines and nuclei, we monitored changes in cell shapes and nuclear positions. We find that the ventral furrow...