... Fluorescent Timer Developmental trajectory analysis T-cells Thymus Mouse Medical Research Council http://dx.doi.org/10.13039/501100007155 MR/S000208/1 MR/S037764/1 Biotechnology and Biological Sciences Research Council http://dx.doi.org/10.13039/501100000268 BB/T020970/1 BB...

...-related organoids remain largely underdeveloped. Here, we established organoids from the thymus, the lymphoid organ responsible for T-cell development. We identified conditions enabling mouse thymic epithelial progenitor cell proliferation and development into organoids with diverse cell populations...

...Zhongyao Ma; Seung Woo Kang; Brian G. Condie; Nancy R. Manley ABSTRACT Thymic epithelial cells (TECs) are crucial to the ability of the thymus to generate T cells for the adaptive immune system in vertebrates. However, no in vitro system for studying TEC function exists. Overexpressing...

... investigated a K5.Foxn1 transgene that drives overexpression in mouse TECs, but causes neither hyperplasia nor delay or prevention of aging-related involution. Similarly, this transgene cannot rescue thymus size in Foxn1 lacZ/lacZ mice, which undergo premature involution as a result of reduced Foxn1 levels...

...Pedro Ferreirinha; Ruben G. R. Pinheiro; Jonathan J. M. Landry; Nuno L. Alves ABSTRACT The thymus stroma constitutes a fundamental microenvironment for T-cell generation. Despite the chief contribution of thymic epithelial cells, recent studies emphasize the regulatory role of mesenchymal cells...

... is highly expressed in the thymus and a recent study demonstrated that its constitutive knock out led to a hyperplastic thymus with retention of thymocytes and many mis-splicing events ( Sznajder et al., 2020 ). Mbnl1 was upregulated approximately two-fold in CD69 + DP cells in the absence of Foxa1...

...-positive cells in the thymus, and attenuates Notch1 signalling after β-selection. Pre-TCR Bcl6 Notch Thymus Thymocyte T-cell development αβT cells develop in the thymus, which provides an essential environment for T-cell fate specification and differentiation ( Koch and Radtke, 2011...

.../ Summary: Medullary thymic epithelial cell lineage specification and progenitor formation in the fetal thymus require notch signaling via notch 1 receptors, whereas further differentiation requires notch 1 to be downregulated. Foxn1 Notch Lineage mTEC Thymus Notch signaling is a highly...

...Dong Liu; Anastasia I. Kousa; Kathy E. O'Neill; Paul Rouse; Martyna Popis; Alison M. Farley; Simon R. Tomlinson; Svetlana Ulyanchenko; Francois Guillemot; Philip A. Seymour; Mette C. Jørgensen; Palle Serup; Ute Koch; Freddy Radtke; C. Clare Blackburn ABSTRACT Thymus function depends...

...Virginia E. Bain; Julie Gordon; John D. O'Neil; Isaias Ramos; Ellen R. Richie; Nancy R. Manley The thymus and parathyroids develop from third pharyngeal pouch (3rd pp) endoderm. Our previous studies show that S hh null mice have smaller, aparathyroid primordia in which thymus fate specification...

.... Previous studies have shown that the Hoxa3 null mutant lacks third pharyngeal pouch derivatives, the thymus and parathyroids by E18.5, and organ-specific markers are absent or downregulated during initial organogenesis. Our current analysis of the Hoxa3 null mutant shows that organ-specific domains did...

...Kaitlin A. G. Reeh; Kim T. Cardenas; Virginia E. Bain; Zhijie Liu; Micheline Laurent; Nancy R. Manley; Ellen R. Richie The thymus and parathyroid glands arise from a shared endodermal primordium in the third pharyngeal pouch (3rd pp). Thymus fate is specified in the ventral 3rd pp between E9.5...

...Jennifer R. Gardiner; Abigail L. Jackson; Julie Gordon; Heiko Lickert; Nancy R. Manley; M. Albert Basson The thymus and parathyroid glands are derived from the third pharyngeal pouch endoderm. The mechanisms that establish distinct molecular domains in the third pouch and control the subsequent...

...Julie Gordon; Nancy R. Manley The thymus is the primary organ responsible for generating functional T cells in vertebrates. Although T cell differentiation within the thymus has been an area of intense investigation, the study of thymus organogenesis has made slower progress. The past decade...

...Tadashi Okubo; Akinori Kawamura; Jun Takahashi; Hisato Yagi; Masae Morishima; Rumiko Matsuoka; Shinji Takada The pharyngeal apparatus is a transient structure that gives rise to the thymus and the parathyroid glands and also contributes to the development of arteries and the cardiac outflow tract...

.... We show that some organs, e.g. the thymus, require Tbx1 expression in the mesoderm and in the epithelia. In addition, these experiments revealed that different pharyngeal arches require Tbx1 in different tissues. Finally, we show that Tbx1 in the mesoderm is required to sustain cell proliferation...

... of TCR + cells appeared in OP9-DL1 co-cultures ( Fig. 2A ). T lineage progression was also demonstrable with respect to CD4 and CD8 expression (data not shown). Given that TCRγδ + cells normally constitute the first wave of lymphopoiesis in the fetal thymus( Havran and Allison, 1988...

...-mail: [email protected] ) 4 9 2003 © The Company of Biologists Limited 2003 2003 Cardiovascular development Pharyngeal arch FGF8 Endoderm Heart field Pharyngeal arch artery Congenital heart disease Vasculogenesis Aortic arch Outflow tract Coronary artery Thymus Parathyroid...

.... H. Ingbar), pp. 303-317. New York: Harper and Row. Smith, C. ( 1965 ). Studies on the thymus of the mammal XIV: Histology and histochemistry of embryonic and early postnatal thymuses of C57BL/6 and AKR strain mice. Am. J. Anat . 116 , 611 -630. Strachan, T. and Read, A. P. ( 1994 ). PAX...

... CD44 − CD25 + , influences the development of epithelial cells, critically inducing the extra, third dimension in the organization of the epithelial cells in the cortex. This tertiary configuration of the thymic epithelium is a typical feature for the thymus, enabling lymphostromal interaction during T...