... and Tcf21-CreER + stromal cells in the testis, but Sertoli cells are the only physiologically important source of SCF that contributes to spermatogenesis. Spermatogenesis Stem cell factor Sertoli cell Testis Mammalian spermatogenesis is a complex developmental process based on a robust...

... determination to determine the source of the center-biased pattern of testis fate disposition in XY gonad development. Hypotheses of center-first expression and paracrine relay were ruled out by the patterns of SRY + and SOX9 + cells, which appear first in a widespread pattern and then exhibit a bias towards...

... Male Contraceptive Initiative S10-OD021615 National Institutes of Health http://dx.doi.org/10.13039/100000002 HD090083 HD090007 HD105963 Testis Spermatogenesis Meiosis Spermatogonia Spermatocytes Summary: Here, we report robust and reliable tools to model mammalian...

... Sox3 Testis National Key Research and Development Program of China 2018YFC1003504 Priority Academic Program Development of Jiangsu Higher Education Institutions Yangzhou University http://dx.doi.org/10.13039/501100007062 XKYCX18_111 In mammalian...

... of proteins mediates movement of specific cargo proteins when bound to karyopherin β. Karyopherin α genes have distinct expression patterns in mouse testis, implying they may have unique roles during mammalian spermatogenesis. Here, we use a loss-of-function approach to determine specifically the role...

... by The Company of Biologists Ltd 2020 http://www.biologists.com/user-licence-1-1/ Summary: Zebrafish Nedd8 plays an important role in controlling ovarian maturation through suppressing androgen receptor activity. Nedd8 Zebrafish Androgen receptor Ovary Testis Neddylation Total RNA...

...Aya Uchida; Sadman Sakib; Elodie Labit; Sepideh Abbasi; R. Wilder Scott; T. Michael Underhill; Jeff Biernaskie; Ina Dobrinski ABSTRACT In mammalian testis, contractile peritubular myoid cells (PMCs) regulate the transport of sperm and luminal fluid, while secreting growth factors and extracellular...

...). Genes involving ‘integrin-mediated signaling’ and ‘vessel development’ are enriched in SSC-s1 ( Fig. S4B,C ), suggesting these processes may play roles in SSC development. Fig. 3. Undifferentiated SG clusters during the SSC establishment period. (A) UMAP plot of SG from P7 mouse testis. Number...

...John E. La Marca; Sarah T. Diepstraten; Amy L. Hodge; Hongyan Wang; Adam H. Hart; Helena E. Richardson; W. Gregory Somers ABSTRACT One fundamental property of a stem cell niche is the exchange of molecular signals between its component cells. Niche models, such as the Drosophila melanogaster testis...

...Ellen K. Velte; Bryan A. Niedenberger; Nicholas D. Serra; Anukriti Singh; Lorena Roa-DeLaCruz; Brian P. Hermann; Christopher B. Geyer; Allon Klein; Barbara Treutlein ABSTRACT In the mammalian testis, sustained spermatogenesis relies on spermatogonial stem cells (SSCs); their progeny either remain...

... and highlighting JAK/STAT pathway functions in proliferation, survival and cell competition that are conserved between Drosophila and vertebrates. upd continues to be produced by niche cells in the adult testis but declines with age ( Kiger et al., 2001 ; Toledano et al., 2012 ; Tulina and Matunis, 2001...

... relationship between FLCs and ALCs has not been clarified yet. Here, we reveal that a subset of FLCs dedifferentiate at fetal stages to give rise to ALCs at the pubertal stage. Moreover, the dedifferentiated cells contribute to the peritubular myoid cell and vascular pericyte populations in the neonatal testis...

.../ Summary: Dynamic cytoplasmic projections connect mammalian spermatogonia and provide patent connections for the sharing of macromolecules during testis development. TEX14 Intercellular bridge Spermatogenesis Spermatogonia Testis Mouse Baboon The foundation of mammalian spermatogenesis...

.... Additionally, blocking RA signaling in Leydig cells resulted in increased permeability of the blood-testis barrier, decreased levels of the steroidogenic enzyme cytochrome P450 17a1 and decreased testosterone levels. Surprisingly, the epididymides of the mutant mice also displayed an abnormal phenotype...

... cyst stem cells (CySCs) in the Drosophila testis is actively promoted by PI3K/Tor signaling, as CySCs lacking PI3K/Tor activity cannot differentiate properly. We find that an insulin peptide produced by somatic cells immediately outside of the stem cell niche acts locally to promote somatic...

...Chang Liu; Karina Rodriguez; Humphrey H.-C. Yao Testis morphogenesis is a highly orchestrated process involving lineage determination of male germ cells and somatic cell types. Although the origin and differentiation of germ cells are known, the developmental course specific for each somatic cell...

... synthesis and degradation. A group of genes that are required for mRNA splicing and protein translation contributes to both GSC self-renewal and early germ cell differentiation. Loss of genes in the protein degradation pathway in cyst cells leads to testis tumors consisting of overproliferated germ cells...

... , B. L. , Goodfellow , P. N. and Fellous , M. ( 1990 ). Genetic evidence equating SRY and the testis-determining factor . Nature   348 , 448 - 450 . 10.1038/348448A0 Beverdam , A. , Wilhelm , D. and Koopman , P. ( 2003 ). Molecular characterization of three gonad...

... development and disease progression. However, its function in adult stem cell maintenance is largely unknown. Here, we report the function of SUMOylation in somatic cyst stem cell (CySC) self-renewal in adult Drosophila testis. The SUMO pathway cell-autonomously regulates CySC maintenance. Reduction...

... histone genes by retinoic acid signaling contributes to spermatogonial differentiation in mice. Spermatogonial differentiation Spermatogenesis Retinoic acid Dominant-negative retinoic acid receptor Testis Replication-dependent core histone genes Spermatogenesis is a highly organized...