... in the leftward curvature of the stomach. To elucidate the molecular events that shape this archetypal laterality, we performed transcriptome analyses of the left versus right sides of the developing stomach in frog embryos. Besides the known LR gene pitx2 , the only gene found to be expressed asymmetrically...

... body axis are beginning to be understood, the mechanisms that shape the asymmetries of individual organs remain less clear. Here, we summarize new evidence challenging century-old ideas about the development of stomach and intestine laterality. We compare classical and contemporary models of asymmetric...

... - and Lgr5 -expressing cells are gastric progenitors in the developing mouse stomach. We show that Axin2 + cells represent a transient population of embryonic epithelial cells in the forestomach. Lgr5 + cells generate both glandular corpus and squamous forestomach organoids ex vivo . Only Lgr5 + progenitors...

... rotation of the intestine is directed by forces outside the gut, but the morphogenetic events that generate anatomical asymmetry in other regions of the digestive tract remain unknown. Here, we show in mouse and Xenopus that the mechanisms that drive the curvature of the stomach are intrinsic to the gut...

... the transmembrane glycoprotein Trop2 as a marker. Here, we report the characterization of Trop2-expressing cells from fetal pre-glandular stomach, growing as immortal undifferentiated spheroids, and their relationship with gastric development and regeneration. Trop2 + cells generating gastric spheroids differed...

... and function. We reveal part of the regulatory machinery responsible for the onset of Xlox and Cdx transcription, uncover a Wnt10 signal that mediates Xlox repression in the intestinal cells, and provide evidence of Xlox - and Cdx -mediated control of stomach and intestine differentiation, respectively. Our...

... ) * These authors contributed equally to this work † Present address: Massachusetts General Hospital, Boston, MA 02114, USA Competing interests statement The authors declare no competing financial interests. 13 5 2011 © 2011. Hedgehog Intestine Stomach Endoderm...

... ) Competing interests statement The authors declare no competing financial interests. 12 3 2010 © 2010. 2010 Hedgehog Intestine Stomach Endoderm Mesoderm Mouse In vertebrate embryos, the visceral endoderm recruits adjacent splanchnic mesoderm to form a primitive gut tube...

... of approximately 1000 TF genes in gastrointestinal development. Despite distinctive structures and functions, the stomach and intestine exhibit limited differences in TF genes. Among differentially expressed transcripts, a few are virtually restricted to the digestive tract, including Nr2e3,previously regarded...

...Yoshimasa Matsuda; Yoshio Wakamatsu; Jun Kohyama; Hideyuki Okano; Kimiko Fukuda; Sadao Yasugi During development of the chicken proventriculus (glandular stomach), gut endoderm differentiates into glandular and luminal epithelium. We found that Delta1- expressing cells, undifferentiated cells...

.... The generation of a lacZ knock-in allele in the COUP-TFII locus in mice allows us to use X-gal staining to follow the expression of COUP-TFII in the developing stomach. We found COUP-TFII is expressed in the mesenchyme and the epithelium of the developing stomach. Conditional ablation of floxed COUP-TFII by Nkx3...

... equally to this work 8 5 2004 © 2004. 2004 SOX SOX9 BMP Gremlin Stomach Pyloric sphincter Differentiation Chick The vertebrate gastrointestinal (GI) tract is a remarkably complex, three dimensional, specialized and vital organ system derived from a simple tubular...

... patterning using the Hoxa5 –/– mice as a model. Hoxa5 is expressed in a dynamic fashion in the mesenchymal component of the developing gut. Its loss of function results in gastric enzymatic anomalies in Hoxa5 –/– surviving mutants that are due to perturbed cell specification during stomach development...

... and at the same times as the endogenous Hoxb-1 gene. However, a transgene construct with point mutations in the DR 5 RARE (DR 5 mu) was not expressed in the developing foregut, which gives rise to organs such as the esophagus, lung, stomach, liver and pancreas. Like the wild-type transgene, this DR 5 RARE mutated...

..., along with a dramatic reduction in Schwann cells, enteric ganglia and adrenal chromaffin cells, suggests a generalized effect on the neural crest. Numerous organs, including the stomach and pancreas also exhibited anomalous development. * Author for correspondence (e-mail: [email protected]...

... experiences aberrant growth and a coincident repression of hydrochloric acid production. Here we show that ectopically expressed TGFα induces an age-dependent cellular reorganization of the transgenic stomach, in which the surface mucous cell population in the gastric pit is greatly expanded at the expense...

... embryogenesis using three different techniques: RT-PCR, northern blots and immunohistochemistry. Our results indicate that Muc-1 expression correlates with epithelial differentiation in stomach, pancreas, lung, trachea, kidney and salivary glands. Once started, Muc-1 synthesis continually increases with time...

... when these were tested on the medium over cultured stomach, trophoblast, adrenal, kidney and brain. The anti-LDL antibody precipitated major labelled molecules with apparent relative molecular masses of 28 ×10 3 , and >200 ×10 3 . The mobilities of these proteins correspond with the respective...