... into oocytes. These injected nuclei were remodeled and reprogrammed using RRFs, and then RRFs were removed by subsequent deletion of somatic nuclei from oocytes. The size of the metaphase II spindle reduced immediately, but did recover when transferred into fresh oocytes. Though affected, the full-term...

..., localizes to ciliary axonemes and basal bodies, and is required for ciliogenesis. We also demonstrate that Dyrk1a localizes to mitotic spindles and that its inhibition leads to decreased forebrain size, abnormal cell cycle progression and cell death during brain development. These findings provide...

...Elizabeth Vargas; Karen P. McNally; Daniel B. Cortes; Michelle T. Panzica; Brennan M. Danlasky; Qianyan Li; Amy Shaub Maddox; Francis J. McNally ABSTRACT Meiotic spindles are positioned perpendicular to the oocyte cortex to facilitate segregation of chromosomes into a large egg and a tiny polar...

... spindle assembly. Also, mechanistic studies demonstrated that WDR62 interacts with centrosome-associated protein CEP170, and deletion of Wdr62 causes downregulation of the CEP170 protein, which in turn leads to the aberrant spindle assembly. In summary, this study indicates that the meiosis of first wave...

... cell division depends on the positioning of the mitotic spindle relative to an axis of polarity. Recent studies have illuminated an expanding list of spindle orientation regulators, and a molecular model for how cells couple cortical polarity with spindle positioning has begun to emerge. Here, we...

... errors by detaching incorrect microtubule-kinetochore interactions. Here, we examine whether MCAK is involved in spindle function in mouse oocyte meiosis I, and whether MCAK is necessary to prevent chromosome segregation errors. We find that MCAK is recruited to centromeres, kinetochores and chromosome...

...François Prodon; Janet Chenevert; Céline Hébras; Rémi Dumollard; Emmanuel Faure; Jose Gonzalez-Garcia; Hiroki Nishida; Christian Sardet; Alex McDougall Mitotic spindle orientation with respect to cortical polarity cues generates molecularly distinct daughter cells during asymmetric cell division...

... ale oko Dynactin Photoreceptor Glia Eye Retina Retinotectal Hair cell Axon Polarity Spindle Microtubules play many essential roles in the structure and function of most, if not all, cells. As a component of the cytoskeleton, they provide structural support for morphological features...

...Greg FitzHarris Microtubules within meiotic and mitotic spindles continually move towards spindle poles in a process termed poleward flux, which is essential for spindle integrity and faithful chromosome segregation. Kinesin 5 is a longstanding candidate for a molecular motor that might drive...

...Bernhard Strauss; Richard J. Adams; Nancy Papalopulu The process of oriented divisions of polarised cells is a recurrent mechanism of cell fate diversification in development. It is commonly assumed that a specialised mechanism of spindle alignment into the axis of polarity is a prerequisite...

...Changbin Chen; Adam Marcus; Wuxing Li; Yi Hu; Jean-Philippe Vielle Calzada; Ueli Grossniklaus; Richard J. Cyr; Hong Ma The spindle plays a central role in chromosome segregation during mitosis and meiosis. In particular, various kinesins are thought to play crucial roles in spindle structure...

... segregation and spindle organization during the syncytial cleavage divisions. In these embryos the syncytial divisions proceed as far as the twelfth cycle, and embryos fail to cellularize. Aberrant divisions and nuclear fusions occur in the early cycles of the nuclear divisions, and become more prominent...

... gene family expressed during gametogenesis. We find that spindlin associates with the meiotic spindle and is modified by phosphorylation in a cell-cycle-dependent fashion. Furthermore, it comigrates with the previously described 30×10 3 M r metaphase complex which is post-translationally modified...

..., that the microtubules of the array rotate with the cytoplasm. © 1988 by Company of Biologists 1988 cortical rotation microtubule sperm aster spindle Xenopus The unfertilized frog egg is organized with radial symmetry about an animal-vegetal axis. The bilateral symmetry of the tadpole...

... induces the formation of tetraploid oocytes. After the separation of chromosomes in anaphase, two spindles start to assemble. However, they merge rapidly and a single meiotic spindle forms. During the transition between metaphase I and metaphase II, in the presence of cytochalasin D, a drop in histone...