...Stefani Gjorcheska; Sandhya Paudel; Sarah McLeod; David Paulding; Louisa Snape; Karen Camargo Sosa; Cunming Duan; Robert Kelsh; Lindsey Barske ABSTRACT Heterozygous variants in SOX10 cause congenital syndromes affecting pigmentation, digestion, hearing, and neural development, primarily...

... nuclear receptors as intrinsic activators of the ectomesenchyme program: zebrafish nr2f5 single and nr2f2;nr2f5 double mutants show marked delays in upregulation of ectomesenchyme genes, such as dlx2a , prrx1a , prrx1b , sox9a , twist1a and fli1a , and in downregulation of sox10 , which is normally...

...% of the enteric neurons and ∼90% of the Sox10-positive ENCDCs therein at 7 dpa were generated through proliferation. Thus, ENS regeneration involves proliferation, entrance and neurogenesis of ENCDCs. This is the first report regarding the regeneration process of the zebrafish ENS. Our findings provide a basis...

...., 2008 ); Tg(pax2a:GFP) , abbreviated as pax2a :GFP ( Picker et al., 2002 ); Tg(sox10(7.2):mRFP) , abbreviated as sox10 :mRFP ( Kirby et al., 2006 ); and Tg(mbp:EGFP) , abbreviated as mbp :GFP ( Almeida et al., 2011 ). Male and female fish were used for the experiments. Larvae were...

... pathways, and exposure of the cells to pertinent growth factors led to the expression of the Schwann cell markers SOX10, KROX20 (EGR2), p75NTR (NGFR), MBP and S100B by day 4 in virtually all cells, and maturation was completed by 2 weeks of differentiation. Gene expression profiling demonstrated expression...

... the expression of Sox10 to promote the formation of secretory acinar lobes in the lacrimal gland. Sox9 and FGF signaling were required for the expression of cartilage-associated extracellular matrix components during early stage lacrimal gland development. Fgfr2 deletion in the ocular surface epithelium reduced...

... Sp8-positive cells are seen streaming from the dLGE (K) with only scattered Olig2 cells (J), which is similar to control embryos (A-C). ( M-V ) No significant differences were found in Sox10- (control 197.8±6.7; Gsx2 ; Ascl1 KO 159.1±9; Ascl1 KO 160.4±10.9 cells/mm 2 ) and Pdgfrα- (control 162.1...

.... We demonstrate temporal manipulation of Gal4 transgene expression in only primary motoneurons and not secondary motoneurons, heretofore impossible with conventional transgenic approaches. In another example, we follow and analyze neural crest cells that regained sox10 function after deactivation...

...Paola Betancur; Tatjana Sauka-Spengler; Marianne Bronner The otic placode, a specialized region of ectoderm, gives rise to components of the inner ear and shares many characteristics with the neural crest, including expression of the key transcription factor Sox10. Here, we show that in avian...

... transcription factors that control neural crest specification and differentiation are often associated with Waardenburg and related disorders. For example, mutations in SOX10 result in a severe form of Waardenburg syndrome, Type IV, also known as Waardenburg-Hirschsprung disease, characterized by pigmentation...

..., as the speed of CNC cells was indistinguishable from that of wild-type counterparts. We determined that the failure of CNC cells to migrate away from the neural rod correlated with the enhanced expression of two transcription factors, foxd3 and sox10 . These transcription factors have many functions in CNC...

... in enteric neural crest cells (ENCCs) resulting from premature neurogenesis. We found that Sox10 expression, which is normally maintained in ENCC progenitors, was decreased in Pofut1 -null ENCCs. By contrast,the number of ENCCs that expressed Mash1, a potent repressor of Sox10, was increased in the Pofut1...

... system. In the embryonic mouse pancreas, we found Phox2b expressed briefly together with Sox10 along the epithelial-mesenchymal border at E12.5 in cells derived from the neural crest. Downregulation of Phox2b shortly thereafter was dependent upon Nkx2.2 expressed in the adjacent pancreatic epithelium...

.... * Author for correspondence (e-mail: [email protected] ) 4 9 2007 © 2007. 2007 Dkk Kremen LRP6 Mesd Slug Sox10 Wnt Xenopus The canonical Wnt/β-catenin signaling cascade is an evolutionarily conserved and ancient pathway playing major roles during animal...

... a member of the spalt family, Xspalt1 ( Munoz-Sanjuan and Brivanlou,2005 ). Overexpression of other transcription factors has been show to result in ectopic vesicles in other species. Pax6 overexpression generates ectopic lens vesicles ( Altmann et al., 1997 ) and Sox10 can generate ectopic otic...

...Thomas J. Carney; Kirsten A. Dutton; Emma Greenhill; Mariana Delfino-Machín; Pascale Dufourcq; Patrick Blader; Robert N. Kelsh sox10 is necessary for development of neural and pigment cell derivatives of the neural crest (NC). However, whereas a direct role for Sox10 activity has been established...

...Michael O'Donnell; Chang-Soo Hong; Xiao Huang; Raymond J. Delnicki; Jean-Pierre Saint-Jeannet Among the families of transcription factors expressed at the neural plate border, Sox proteins have been shown to regulate multiple aspects of neural crest development. Sox8, Sox9 and Sox10, exhibit...

...Nadege Bondurand; Dipa Natarajan; Amanda Barlow; Nikhil Thapar; Vassilis Pachnis The transcriptional regulator SOX10 and the signalling molecule endothelin 3 have important roles in the development of the mammalian enteric nervous system (ENS). Using a clonal cell culture system, we show that SOX10...

... specification, whereas Sox10 is required for terminal differentiation. We show that oligodendrocytes in the spinal cord additionally express the highly related Sox8. In Sox8-deficient mice, oligodendrocyte development proceeded normally until birth. However, terminal differentiation of oligodendrocytes...

...Stone Elworthy; James A. Lister; Tom J. Carney; David W. Raible; Robert N. Kelsh The transcription factor Sox10 is required for the specification, migration and survival of all nonectomesenchymal neural crest derivatives including melanophores. sox10 -/- zebrafish lack expression...