...Catarina Sequeira; Lou Martha Wackerbarth; Andreia Pena; Mafalda Sá-Pereira; Cláudio A. Franco; Edgar R. Gomes ABSTRACT Skeletal muscle development is a complex process involving myoblast fusion to generate multinucleated fibers. Myonuclei first align in the center of the myotubes before migrating...

...Olga G. Jaime; Jessica Arias; Shreya Pavani; April D. Pyle; Michael R. Hicks ABSTRACT The earliest skeletal muscle progenitor cells (SMPCs) derived from human pluripotent stem cells (hPSCs) are often identified by factors expressed by a diverse number of progenitors. An early transcriptional...

...Yudai Ono; Saundra Schlesinger; Kanako Fukunaga; Shinsei Yambe; Tempei Sato; Takako Sasaki; Chisa Shukunami; Hiroshi Asahara; Masafumi Inui ABSTRACT Movement of the vertebrate body is supported by the connection of muscle, tendon and bone. Each skeletal muscle in the vertebrate body has a unique...

... in actin-related processes at different subcellular locales, all essential for muscle integrity and function. Skeletal muscle Tropomodulin (Tmod) Drosophila Sarcomere length Myofibril orientation Tension-mediating proteins Misshapen nuclei National Institutes of Health R01...

.../10.13039/100000002 R21 AR072511 Skeletal muscle Muscle interstitial cells Hox genes Highlighted Article: Hoxa11 expression marks a population of muscle interstitial cells capable of an extensive satellite cell-independent contribution to skeletal muscle fibers during adult homeostasis...

...-Bankston et al., 2015 ), CDH15 ( Marti et al., 2013 ) and the small fusogenic peptide myomixer ( MYMX ), which in mouse peaks in skeletal muscles at E14.5 ( Bi et al., 2017 ) ( Figs S2 and S3 ). This cluster was located between the PAX7 + MYOG − clusters and a fourth cluster (cluster 4) characterized...

...Megha Agarwal; Akashi Sharma; Pankaj Kumar; Amit Kumar; Anushree Bharadwaj; Masum Saini; Gabrielle Kardon; Sam J. Mathew ABSTRACT Myosin heavy chain-embryonic (MyHC-emb) is a skeletal muscle-specific contractile protein expressed during muscle development. Mutations in MYH3 , the gene encoding MyHC...

...Allison P. Kann; Robert S. Krauss ABSTRACT Skeletal muscle myofibers are large syncytial cells comprising hundreds of myonuclei, and in situ hybridization experiments have reported a range of transcript localization patterns within them. Although some transcripts are uniformly distributed...

.... ( 2003 ). Intrinsic, Hox-dependent cues determine the fate of skeletal muscle precursors . Dev. Cell   5 , 379 - 390 . 10.1016/S1534-5807(03)00263-6 Anderson , C. , Williams , V. C. , Moyon , B. , Daubas , P. , Tajbakhsh , S. , Buckingham , M. E. , Shiroishi , T...

... Skeletal muscle Transcription factors Epigenetics Cell signaling Differentiation National Institutes of Health 10.13039/100000002 1R01EY026202 Australian Research Council 10.13039/501100000923 FT110100573 Flinders Foundation Skeletal muscle owes its...

..., the expression of the myogenic differentiation markers MyoD and myogenin was investigated. As shown in Fig. 2 A,B, an increased number of MyoD + or myogenin + cells was detected in skeletal muscle sections of co-dKO mice. Consistent with Fig. 1 D, a decrease in the number of MuSCs on isolated single...

... skeletal muscle development, where it is a master regulator of the transition from embryonic to foetal myogenesis. Recently, Nfix was shown to be involved in adult muscle regeneration and in muscular dystrophies. Here, we have investigated the signalling that regulates Nfix expression, and show that JunB...

... Body skeletal muscles derive from the paraxial mesoderm, which forms in the posterior region of the embryo. Using microarrays, we characterize novel mouse presomitic mesoderm (PSM) markers and show that, unlike the abrupt transcriptome reorganization of the PSM, neural tube differentiation...

...Amir Mizbani; Edlira Luca; Elisabeth J. Rushing; Jan Krützfeldt MicroRNAs (miRNAs) are important regulators of skeletal muscle regeneration, but the underlying mechanisms are still incompletely understood. Here, comparative miRNA sequencing analysis of myogenic progenitor cells (MPs) and non...

... expression of JMJD3c followed by the forced expression of lineage-defining transcription factors enabled the hPSCs to activate tissue-specific genes directly. We have also shown that the introduction of JMJD3c facilitates the differentiation of hPSCs into functional hepatic cells and skeletal muscle cells...

... to reflect the localization of active canonical Wnt signaling but without itself being of any obvious importance for skeletal muscle under steady-state conditions. Although general muscle biology is not affected by the absence of Axin2, muscle fibers with active Wnt signaling differ morphologically from...

...: Satellite cell depletion prevents fiber hypertrophy in the plantaris and extensor digitorum longus muscles in mice, suggesting that satellite cells are obligatory for hypertrophic growth. Hypertrophy Plasticity Satellite cells Skeletal muscle Stem cells Mouse Norges Forskningsråd...

...Nicolas A. Dumont; Yu Xin Wang; Michael A. Rudnicki Muscle stem cells, termed satellite cells, are crucial for skeletal muscle growth and regeneration. In healthy adult muscle, satellite cells are quiescent but poised for activation. During muscle regeneration, activated satellite cells transiently...

... development Satellite cell differentiation Satellite cell quiescence Skeletal muscle Mouse Human Stem cells reside in specialized environments that regulate their behavior, called stem cell niches. Myogenic stem cells called satellite cells (mSCs) are involved in muscle growth and in regeneration...

... in vivo tagging of the nuclear envelope with biotin and the subsequent affinity purification of the labeled nuclei. In this study we successfully purified nuclei of cardiac and skeletal muscle from Xenopus using this strategy. We went on to demonstrate the utility of this approach by coupling the INTACT...