...Abdull J. Massri; Laura Greenstreet; Anton Afanassiev; Alejandro Berrio; Gregory A. Wray; Geoffrey Schiebinger; David R. McClay ABSTRACT Using scRNA-seq coupled with computational approaches, we studied transcriptional changes in cell states of sea urchin embryos during development to the larval...

... Dorsoventral patterning Sea urchin embryo Ministry of Science and Technology, Taiwan 10.13039/501100004663 104-2627-B-001-001 103-2311-B-001-030-MY3 105-2321-B-001-060 Oxygen is essential for the survival of aerobic organisms. In animal cells, several signaling pathways can...

...Michael L. Piacentino; Janani Ramachandran; Cynthia A. Bradham Skeletal patterning in the sea urchin embryo requires a conversation between the skeletogenic primary mesenchyme cells (PMCs) and the overlying pattern-dictating ectoderm; however, our understanding of the molecular basis...

...Vincenzo Cavalieri; Rosa Guarcello; Giovanni Spinelli In the indirect developing sea urchin embryo, the primary mesenchyme cells (PMCs) acquire most of the positional and temporal information from the overlying ectoderm for skeletal initiation and growth. In this study, we characterize the function...

...Tara Sharma; Charles A. Ettensohn The well-known regulative properties of the sea urchin embryo, coupled with the recent elucidation of gene regulatory networks (GRNs) that underlie cell specification, make this a valuable experimental model for analyzing developmental plasticity. In the sea urchin...

... © 2010. 2010 Sea urchin embryo Endomesoderm Nodal BMP2/4 Activin FoxA FoxD sm30 GATA1/2/3 LMO2 ese gcm Pigment cells Blastocoelar cells In vertebrates, Nodal factors play conserved roles in the induction of the mesoderm and endoderm, in the formation of a dorsally...

...Charles A. Ettensohn; Chisato Kitazawa; Melani S. Cheers; Jennifer D. Leonard; Tara Sharma Cell fates in the sea urchin embryo are remarkably labile, despite the fact that maternal polarity and zygotic programs of differential gene expression pattern the embryo from the earliest stages. Recent work...

... evidence that during early development of the sea urchin embryo, NLK is expressed in the mesodermal precursors in response to Notch signalling and directs their fate by downregulating TCF. The expression pattern of nlk is strikingly similar to that of Delta and the two genes regulate the expression of each...

... study, we have exploited the optical transparency of the sea urchin embryo to measure the half-life of β-catenin in specific cell lineages in vivo. We report a gradient in β-catenin stability along the animal-vegetal (A-V) axis during cleavage and show that degradation ofβ-catenin in animal blastomeres...

...Eric Röttinger; Lydia Besnardeau; Thierry Lepage In the sea urchin embryo, the skeleton of the larva is built from a population of mesenchymal cells known as the primary mesenchyme cells (PMCs). These derive from the large micromeres that originate from the vegetal pole at fourth cleavage...

...Charles A. Ettensohn; Michele R. Illies; Paola Oliveri; Deborah L. De Jong In the sea urchin embryo, the large micromeres and their progeny function as a critical signaling center and execute a complex morphogenetic program. We have identified a new and essential component of the gene network...

...Xiaodong Zhu; Gregory Mahairas; Michele Illies; R. Andrew Cameron; Eric H. Davidson; Charles A. Ettensohn The primary mesenchyme cells (PMCs) of the sea urchin embryo have been an important model system for the analysis of cell behavior during gastrulation. To gain an improved understanding...

...Eric W. Howard; Laurel A. Newman; David W. Oleksyn; Robert C. Angerer; Lynne M. Angerer ABSTRACT Localization of nuclear β-catenin initiates specification of vegetal fates in sea urchin embryos. We have identified SpKrl , a gene that is activated upon nuclear entry of β-catenin. SpKrl...

...Zheng Wei; Lynne M. Angerer; Robert C. Angerer Because the transcription of the SpHE gene is regulated cell-autonomously and asymmetrically along the maternally determined animal-vegetal axis of the very early sea urchin embryo, its regulators provide an excellent entry point for investigating...

...Kirsten A. Guss; Charles A. Ettensohn ABSTRACT The skeleton of the sea urchin embryo is synthesized by the primary mesenchyme cells (PMCs). Previous studies have shown that local interactions between PMCs and the neighboring ectoderm regulate several aspects of skeletal morphogenesis, including PMC...

... gene regulation sea urchin embryo Endo16 gene cis -regulatory system The cis -regulatory systems that control spatial and temporal gene expression are typically composed of subelements that function positively or negatively in different embryonic territories, or are utilized in different...

... is initially specified, as defined above. However, these Endo16 -positive cells are not completely specified, as indicated by their greatly delayed and morphologically abnormal gastrulation. 14 06 1995 © 1995 by Company of Biologists 1995 sea urchin embryo blastomere specification cell...

... . * Author for correspondence (e-mail: [email protected] ) 15 03 1995 © 1995 by Company of Biologists 1995 tektin cilia ciliogenesis length regulation sea urchin embryo messenger RNA Ciliogenesis and flagellar development are cellular events marked by coordinated gene...

...Sheng-Ping L. Hwang; Jacqueline S. Partin; William J. Lennarz ABSTRACT A cDNA clone encoding a protein homologous to human bone morphogenetic protein 1 (huBMP1) was isolated from a sea urchin embryo cDNA library. This sea urchin gene, named suBMP , encodes a protein of M r of 72×10 3 . The deduced...

... that regulates the rate of skeletal rod elongation. * Author for correspondence 28 05 1993 © 1993 by Company of Biologists 1993 sea urchin embryo mesoderm primary mesenchyme skeletogenesis morphogenesis size regulation Studies of morphogenesis seek to identify...