... mesenchyme is absent. Lineage tracing demonstrates the previously unknown developmental origin of the mesenchyme, the sclerotome, which also gives rise to the spine. Strikingly, we find that there is significantly less sclerotome in smb than in wild type. Our results give insight into the origin...

... known as pdgfaa ) and pdgfαb (also known as pdgfab ) or blocking PDGF signaling with an inhibitor impairs erythroid progenitor differentiation, thus resulting in a significant decrease in the number of erythrocytes. We reveal that pdgfαb is expressed in sclerotomal cells, and that its receptor genes...

... ichthyologists, paleontologists and developmental biologists and discusses the mechanisms that regulate and diversify vertebral patterning. Bone Notochord Sclerotome Segmentation Vertebrae The vertebral column is the defining feature of vertebrates and comprises a segmented/repeat series...

... using such small molecules were chondrogenic in vitro , and expressed trunk paraxial mesoderm markers such as Tcf15 and Meox1, and somite markers such as Uncx, but failed to express sclerotome markers such as Pax1. Induction of the osteochondrogenically committed sclerotome from somite requires sonic...

... the effects of BMP signaling on the specification of the sclerotome. We confirmed reduction of Pax1 and Pax9 expression in Nog mutants, but found that Nog ; Grem1 double mutants completely fail to initiate sclerotome development. Furthermore, Nog mutants that also lack one allele of Grem1 exhibit a dramatic...

... migration and segmentation of the peripheral nervous system are separable processes. * Author for correspondence (e-mail: [email protected] ) 27 10 2005 © 2006. 2006 Trunk neural crest migration Sclerotome Neuropilin 2 Semaphorin 3F Mouse Chick The neural...

... compartments, the myotome and sclerotome, in response to signals secreted from surrounding tissues. As the somite matures, the tendon lineage is established within the dorsolateral sclerotome, adjacent to and beneath the myotome. We examine interactions between the three lineages by observing tendon...

...Ava E. Brent; Clifford J. Tabin During somite development, a fibroblast growth factor (FGF) signal secreted from the myotome induces formation of a scleraxis ( Scx )-expressing tendon progenitor population in the sclerotome, at the juncture between the future lineages of muscle and cartilage. While...

... migration Convergence extension Adaxial cells Slow muscle Sclerotome Germ cells Metastasis Zebrafish Hyaluronan (HA) is a linear polysaccharide of high molecular weight,consisting of glucuronic acid (GlcA) and N -acetyl-glucosamine(GlcNAc) disaccharide repeats, linked by alternating β1-3...

.... In the early chicken embryo CPZ is initially expressed throughout the somites and subsequently becomes restricted to the sclerotome. To initiate a functional analysis of CPZ, a CPZ producing retroviral vector was applied to the presomitic mesoderm at the level of the future wing. This resulted in a loss...

... and their target genes remain to be elucidated. We show, by analyzing Pax1;Pax9 double mutant mice, that expression of Bapx1 in the sclerotome requires the presence of Pax1 and Pax9, in a gene dose-dependent manner. By using a retroviral system to overexpress Pax1 in chick presomitic mesoderm explants, we show...

... vertebral column cellular and developmental biology. In this study, we characterized alignment of somites and vertebrae, distribution of individual sclerotome progeny along the anteroposterior axis and development of the axial skeleton in zebrafish. Our clonal analysis of zebrafish sclerotome shows...

... embryo are scattered throughout the entire structure. In both cases, short pulses of radiolabeled thymidine were delivered followed by immediate fixation thus reflecting intrinsic cell proliferation. D, dermis; M, myotome, S, sclerotome. Scale bar: 30 μm in A; 70 μm in B. Fig. 1. Mitotically competent...

... by an accumulation of Pax1 -expressing cells. (2) The scapular head and neck are of lateral plate mesodermal origin. (3) In contrast, the scapular blade is composed of somitic cells. (4) Unlike the Pax1 -positive cells of the vertebral column, which are of sclerotomal origin, the Pax1 -positive cells of the scapular...

...Ahmed Mansouri; Anne K. Voss; Tim Thomas; Yoshifumi Yokota; Peter Gruss ABSTRACT The expression of the homeobox gene Uncx4 . 1 in the somite is restricted to the caudal half of the newly formed somite and sclerotome. Here we show that mice with a targeted mutation of the Uncx4 . 1 gene exhibit...

...Jennifer Dockter; Charles P. Ordahl ABSTRACT We have repeated classic dorsoventral somite rotation experiments ( Aoyama and Asamoto, 1988 , Development 104, 15-28) and included dorsal and ventral gene expression markers for the somitogenic tissue types, myotome and sclerotome, respectively. While...

...Ruijin Huang; Qixia Zhi; Corina Schmidt; Jörg Wilting; Beate Brand-Saberi; Bodo Christ* ABSTRACT The somites of vertebrate embryos give rise to sclerotomes and dermomyotomes. The sclerotomes form the axial skeleton, whereas the dermomyotomes give rise to all trunk muscles and the dermis of the back...

... the sclerotome, while the dorsally located surface ectoderm and dorsal neural tube provide the dorsalizing signals to specify the dermomyotome. Noggin and SHH-N have been implicated as the ventralizing signals produced by the notochord. Members of the WNT family of proteins, on the other hand, have been...

... cells Sclerotome Somite Ventrolateral lip Skeletal muscles of vertebrate embryos are generated from the metameric somites. The latter structures are initially epithelial, and in response to signals from their local environment, become specified into a dorsal dermomyotome and a ventral...

... of the developing limb, extending its role in axon pathfinding to the distal periphery. * Author for correspondence (e-mail: [email protected] ) 26 08 1998 © 1998 by Company of Biologists 1998 Chick Eph EphA7 Cek11 Ephrins Neural tube Axon guidance Sclerotome Limb patterning...