... or predetermined subpopulation of VAE, as well as the molecular mechanism underlying this process, remain unclear. We previously reported that different zebrafish stem cell leukemia ( scl ) isoforms are differentially required for HSC formation in the ventral wall of the dorsal aorta. However, the exact stage...

... cell leukaemia (Scl/Tal1) is crucial for development of these adult haemangioblasts in Xenopus and establish the regulatory cascade controlling its expression. We show that VEGFA produced in the somites is required to initiate adult haemangioblast programming in the adjacent DLP by establishing...

... progenitors marked by another bHLH factor, Scl . This is achieved by crosstalk with the BMP and Wnt pathways,which are involved in mesoderm ventralization and SMC lineage induction,respectively. Our findings reveal a novel role of the Notch pathway in early ventral mesoderm differentiation, and suggest...

... within the enhancer diminished its activity, the ablation of its single E box extinguished endothelial enhancer-directed expression in transgenic mice. Development of the endothelium is known to require SCL(TAL1), and an SCL-E12 (SCL-Tcfe2a) heterodimer can bind the crucial E box in the enhancer in vitro...

...-CFC-enriched transcripts, was further characterized by utilizing the in vitro model of ES cell differentiation. Our studies revealed that Gata2 was a direct target of BMP4 and that enforced GATA2 expression upregulated Bmp4, Flk1 and Scl . Conditional GATA2 induction resulted in a temporal-sensitive...

..., migration, and lobulation. Dev. Dyn . 235 , 444 -445. Ferrara, N. ( 2004 ). Vascular endothelial growth factor: basic science and clinical progress. Endocr. Rev. 25 , 581 -611. Gering, M., Rodaway, A. R., Gottgens, B., Patient, R. K. and Green, A. R. ( 1998 ). The SCL gene specifies...

...Changwon Park; Iva Afrikanova; Yun Shin Chung; Wen Jie Zhang; Elizabeth Arentson; Guo hua Fong; Alexander Rosendahl; Kyunghee Choi The receptor tyrosine kinase FLK1 and the transcription factor SCL play crucial roles in the establishment of hematopoietic and endothelial cell lineages in mice. We...

...Richard Martin; Rachid Lahlil; Annette Damert; Lucile Miquerol; Andras Nagy; Gordon Keller; Trang Hoang During development, hematopoiesis initiates in the yolk sac through a process that depends on VEGF/Flk1 signaling and on the function of the SCL/Tal1 transcription factor. Here we show that VEGF...

... lineages, we have generated knock-in embryonic stem (ES) cells carrying a non-functional human CD4 at the Scl locus. By using in vitro differentiated Scl +/CD4 ES cells, we demonstrate that FLK1 and SCL are molecular determinants of the hemangioblast. Furthermore, our studies demonstrate that hematopoietic...

.... Expression analysis of hematopoietic genes suggests that formation of primitive hematopoietic precursors is deficient in bls mutants and those few blood precursors that are specified fail to differentiate and undergo apoptosis. Overexpression of scl , but not bmp4 or gata1 , can lead to partial rescue...

...Paul E. Mead; Anne E. Deconinck; Tara L. Huber; Stuart H. Orkin; Leonard I. Zon Hematopoietic stem cells are derived from ventral mesoderm during vertebrate development. Gene targeting experiments in the mouse have demonstrated key roles for the basic helix-loop-helix transcription factor SCL...

... of the basic helix-loop-helix gene SCL (stem cell leukemia) causes a block early in the hematopoietic program with defects in all hematopoietic lineages. To understand how SCL participates in the organogenesis of blood, we have isolated cDNAs encoding Xenopus SCL and characterized the function of SCL during...