... that pseudotyped, replication-incompetent retroviruses can be used in axolotls to permanently express markers or genetic elements for functional study. These viruses, when modified by changing their coat protein, can infect axolotl cells only when they have been experimentally manipulated to express the receptor...

...+ -binding protein, S100β, in those cells. Introduction of the mouse Gcm1 gene in cultured cells from embryonic brains resulted in the induction of an astrocyte lineage. This effect was also observed by in utero injection of retrovirus harboring mouse Gcm1 into the embryonic brain. However, cultures from...

.... Retrovirus-mediated overexpression of ET A in ventricular myocytes in which endogenous ET receptors had been downregulated, enhanced their responsiveness to ET, allowing them to differentiate into conduction cells. These results suggest that the developmentally regulated expression of ET receptors plays...

... for correspondence (e-mail: [email protected] ) 27 05 2000 20 07 2000 © 2000 by Company of Biologists 2000 Heart development Cardiac conduction system Purkinje fiber Endothelin converting enzyme Retrovirus The rhythmic heart beat is regulated by a precisely timed...

... prestreak stages XI-XII through HH stage 3, when the primitive streak is initially established and prior to the migration of mesoderm. Using replication-defective retrovirus-mediated gene transfer and vital dye labeling, precursor cells of the stage 3 primitive streak were mapped predominantly to a specific...

... and localized recruitment of multipotent cardiomyogenic cells to the developing network of specialized cardiac tissues. * Author for correspondence (e-mail: [email protected] ) 23 08 1999 21 10 1999 © 1999 by Company of Biologists 1999 Heart Conduction system Retrovirus...

... for the control of VIP expression during the development of cholinergic sympathetic neurons. Cytokine Retrovirus Antisense Choline acetyltransferase Neuropeptide VIP The generation of different types of neurons and their connections in specific circuits is a prerequisite for the function...

[email protected] ) 18 12 1997 04 02 1998 © 1998 by Company of Biologists 1998 Fgf Neural retina Optic vesicle Eye development Retrovirus Vertebrate Development of the vertebrate eye depends on the coordinated interaction of three distinct tissues: neuroepithelium, overlying...

... , A. I. , Terzian , C. , Santamaria , P. , Pélisson , A. , Prud’homme , N. and Bucheton , A. ( 1994 ). Retroviruses in invertebrates: the gypsy retrotransposon is apparently an infectious retrovirus of Drosophila melanogaster . Proc. Natl. Acad. Sci. USA 91 , 1285...

... in and around the sensory organs. Reducing normal death using retrovirus-mediated overexpression of human bcl-2 causes abnormalities in ear morphogenesis: hollowing of the center of each canal is either delayed or fails entirely. These data provide new evidence to explain the role of cell death in morphogenesis...

... in mechanisms triggering terminal differen- tiation of the limb muscle mass during avian embryogen- esis. * Author for correspondence 20 09 1995 © 1996 by Company of Biologists 1996 fibroblast growth factor receptor retrovirus myogenesis cell migration chick limb muscle...

...John F. Engelhardt; Howard Schlossberg; James R. Yankaskas; Lorita Dudus ABSTRACT A bronchial xenograft model of the human airway was used to identify submucosal gland progenitor cells within the surface airway epithelium. Lineage analysis using recombinant retroviruses has demonstrated...

... forming myocardial conduction tissues, two origins, myogenic and neural, have been suggested for this specialized tissue. Using replication-defective retroviruses, encoding recombinant β-galactosidase (β-gal), we have analyzed cell lineage for Purkinje fibers (i.e., the peripheral elements...

... limb development FGF-2 retrovirus pattern formation chick Little is known about the role of fibroblast growth factor2 (FGF-2) in chick limb bud development. Although the limb bud contains significant quantities of FGF-2 ( Seed et al., 1988 ; Munaim et al., 1988 ) and FGF receptors ( Olwin...

... cell types, we used a recombinant retrovirus to label precursor cells in the chick optic tectum with a heritable marker, the E. coli lacZ gene. The progeny of the infected cells were detected at later stages of development with a histochemical stain for the lacZ gene product. Radial glia were...

...Eric Frank; Joshua R. Sanes ABSTRACT We used retrovirus-mediated gene transfer to study the lineage of neural crest cells in chick embryos. Individual crest cells were infected before they migrated from the neural tube, and their clonal progeny were subsequently revealed in sensory ganglia...

... perchlorate), and a replication-deficient retrovirus (LZ10; Galileo et al . 1990 ) were employed as tracers. Since LZ10 was constructed with lacZ of E. coli as a reporter gene, infected cells were identified by demonstrating β -galactosidase immunoreactivity. Dil and LZ10 were injected between the neural tube...

...Christopher P. Austin; Constance L. Cepko The migration patterns of embryonic mouse cortical cells were investigated using a replication-incompetent retrovirus vector (BAG). The lateral ventricles of embryonic day 12 mouse embryos were infected with BAG and brains were harvested 2, 3, 4 and 6 days...

... restriction endonuclease fragments to high specific activity . Analyt. Biochem . 132 , 6 – 13 . 10.1016/0003-2697(83)90418-9 Guild , B. C. , Finer , M. H. , Housman , D. E. and Mulligan , R. C. ( 1988 ). Development of retrovirus vectors useful for expressing genes in cultured...

... of Molecular Hematology, National Institutes of Health, Bethesda, MD 20892, USA † Present address: Department of Biological Chemistry, The Johns Hopkins School of Medicine, Baltimore, MD 21205, USA © 1988 by Company of Biologists 1988 retrovirus embryonal carcinoma cell cDNA cloning gene...