... trajectories in open-book preparations of Syk −/− ; Zap70 −/− mouse embryos, we show that Syk kinases are dispensable for attraction towards the midline but confer growth cone responsiveness to repulsive signals that expel commissural axons from the midline. Known to serve a repulsive function at the midline...

..., and rescue of eye vesicle integrity with simultaneous knockdown of sema6a and plxna2 , point to an eye-autonomous requirement for Sema6a/Plxna2. We propose a novel, tissue-autonomous mechanism of organ cohesion, with neutralization of repulsion suggested as a means to promote interactions between cells...

... at the site of dorsal root entry. Our results suggest that Sema5B contributes to a repulsive barrier for centrally projecting primary sensory axons, forcing them to turn and establish the dorsal funiculus. * Author for correspondence ( [email protected] ) Competing interests The authors declare...

... increased to ∼30% compared with control antibody-treated cells ( Fig. 3E ). These results suggest a reduction in the repulsive interactions between neurites after neutralization of Nogo-A. Similar observations were made in cultures of dissociated Nogo-A KO DRG neurons ( Fig. 3A ). Genetic ablation...

...Hope A. Coleman; Juan-Pablo Labrador; Rebecca K. Chance; Greg J. Bashaw Slits and their Roundabout (Robo) receptors mediate repulsive axon guidance at the Drosophila ventral midline and in the vertebrate spinal cord. Slit is cleaved to produce fragments with distinct signaling properties...

... influence these patterning and avoidance processes by promoting attractive, repulsive or adhesive responses to specific cues. The Drosophila transmembrane protein Turtle (Tutl) and its orthologs in other species are conserved members of the immunoglobulin superfamily, the in vivo functions of which...

...-Spectrin in midline repulsion. Surprisingly, neither the Ankyrin-binding nor the pleckstrin homology (PH) domains of β-Spectrin are required for accurate guidance decisions. α-Spectrin is dependent upon β-Spectrin for its normal subcellular localization and/or maintenance, whereas α- spectrin mutants...

... the mesenchyme. Here, we have provided in vivo evidence that Slit-Robo signalling is involved in cranial motor axons repulsion from the midline; currently evidence of an in vivo role for netrin 1 and Sema3a in this process is lacking. The relative contribution of different repellent mechanisms remains...

... Beats plays only a small role in generating axonal specificity, secondary to stronger mechanisms such as Fas II-mediated axon adhesion or Semaphorin-mediated repulsion. If the latter is true, then we may be able to observe an effect of the loss of one of the new Beats if we look in a sensitized genetic...

... mutants, although Roundabout remains expressed on crossing axons. Calmodulin and Son of sevenless also regulate axon crossing in a commissureless mutant. These data suggest that Calmodulin and Son of sevenless signaling pathways function to interpret midline repulsive cues which prevent axons crossing...

... collapsin-1 semaphorin-D semaphorin-III spinal cord sensory afferent trk-C antibody repulsion repellent growth cone collapse guidance pathfinding chick The correct wiring together of the nervous system requires that specific axonal growth cones respond appropriately to a variety...

..., the first cells to migrate all choose the medial pathway. High resolution imaging of cells in living embryos suggests that neural crest cells do so because of repulsion by somites: cells take the medial pathway because the lateral somite surface triggers a paralysis and retraction of protrusions (contact...