... sequencing experiments demonstrate that adult spheroid-generating cells belong to a hitherto undescribed developmental lineage, independent of Lgr5-positive CBCs, and are involved in regeneration of the epithelium following CBC ablation. * Present address: Core Unit pluripotent Stem Cells...

...Bryana N. Harris; Laura A. Woo; R. Noah Perry; Alexia M. Wallace; Mete Civelek; Matthew J. Wolf; Jeffrey J. Saucerman ABSTRACT Numerous regulators of cardiomyocyte (CM) proliferation have been identified, yet how they coordinate during cardiac development or regeneration is poorly understood. Here...

... embryonic and post-metamorphic processes, such as regeneration. We adopted a semi-automated aquaculture system for N. vectensis and developed a dietary protocol optimized for the different life stages. Thereby, we increased spawning efficiencies, juvenile growth and survival rates, and considerably reduced...

... in an unbiased way, just as similar studies use scRNA-seq to understand the sequence of events that occur in regeneration ( Kakebeen et al., 2020 ; Lin et al., 2021 ; Hu et al., 2022 ). Still, we have only touched the surface of the transfating sequence. Our dataset currently is too sparse to penetrate deeply...

.../ Summary: Primary intestinal organoids rely on Lgr5 + intestinal stem cells for survival following genotoxic injury induced by doxorubicin treatment, clarifying the cell types necessary for intestinal organoid regeneration in vitro . Doxorubicin Intestinal stem cells Organoids Regeneration...

... zebrafish, can robustly regenerate hair cells after severe organ damage. The zebrafish inner ear provides an understudied model system for understanding hair cell regeneration in organs that are highly conserved with their mammalian counterparts. Here, we quantitatively examine hair cell addition during...

...; Meera T. Saxena; David F. Ackerley; Jiang Qian; Seth Blackshaw; Eric Horstick; Jeff S. Mumm ABSTRACT Many genes are known to regulate retinal regeneration after widespread tissue damage. Conversely, genes controlling regeneration after limited cell loss, as per degenerative diseases, are undefined...

...Mi-Sun Lee; Jonathan Jui; Aresh Sahu; Daniel Goldman ABSTRACT In the injured zebrafish retina, Müller glial cells (MG) reprogram to adopt retinal stem cell properties and regenerate damaged neurons. The strongest zebrafish reprogramming factors might be good candidates for stimulating a similar...

...Can Aztekin ABSTRACT One of the enduring debates in regeneration biology is the degree to which regeneration mirrors development. Recent technical advances, such as single-cell transcriptomics and the broad applicability of CRISPR systems, coupled with new model organisms in research, have led...

...Loïc Bideau; Zoé Velasquillo-Ramirez; Loeiza Baduel; Marianne Basso; Pascale Gilardi-Hebenstreit; Vanessa Ribes; Michel Vervoort; Eve Gazave ABSTRACT The capacity to regenerate lost tissues varies significantly among animals. Some phyla, such as the annelids, display substantial regenerating...

...Douglas Terry; Colby Schweibenz; Kenneth Moberg ABSTRACT Regenerative ability often declines as animals mature past embryonic and juvenile stages, suggesting that regeneration requires redirection of growth pathways that promote developmental growth. Intriguingly, the Drosophila larval epithelia...

...Clayton J. Becker; Valentina Cigliola; Pierre Gillotay; Ashley Rich; Alessandro De Simone; Yanchao Han; Stefano Di Talia; Kenneth D. Poss ABSTRACT Identification of signaling events that contribute to innate spinal cord regeneration in zebrafish can uncover new targets for modulating injury...

...Xiang Li; Minhui Ren; Yunpeng Gu; Tong Zhu; Yu Zhang; Jie Li; Chao Li; Guangqin Wang; Lei Song; Zhenghong Bi; Zhiyong Liu ABSTRACT Cochlear inner hair cells (IHCs) are primary sound receptors, and are therefore a target for developing treatments for hearing impairment. IHC regeneration in vivo has...

...Tyler A. Square; Emma J. Mackey; Shivani Sundaram; Naama C. Weksler; Zoe Z. Chen; Sujanya N. Narayanan; Craig T. Miller ABSTRACT Most vertebrate species undergo tooth replacement throughout adult life. This process is marked by the shedding of existing teeth and the regeneration of tooth organs...

...Yu-Lieh Lin; Ling Yu; Mingquan Yan; Katherine Zimmel; Osama Qureshi; Felisha Imholt; Tao Li; Ivan Ivanov; Regina Brunauer; Lindsay Dawson; Ken Muneoka ABSTRACT A mouse organoid culture model was developed to regenerate articular cartilage by sequential treatment with BMP2 and BMP9 (or GDF2...

...Ahmed I. Mahmoud ABSTRACT Metabolic switches are a crucial hallmark of cellular development and regeneration. In response to changes in their environment or physiological state, cells undergo coordinated metabolic switching that is necessary to execute biosynthetic demands of growth and repair...

...Bidushi Chandra; Matthew G. Voas; Erin L. Davies; Rachel H. Roberts-Galbraith ABSTRACT Glia play multifaceted roles in nervous systems in response to injury. Depending on the species, extent of injury and glial cell type in question, glia can help or hinder the regeneration of neurons. Studying...

...Jonathan D. Louie; Benjamin H. Bromberg; Matthew J. Zunitch; James E. Schwob ABSTRACT Horizontal basal cells (HBCs) residing within severely damaged olfactory epithelium (OE) mediate OE regeneration by differentiating into odorant-detecting olfactory sensory neurons (OSNs) and other tissue...

... was suppressed by rapamycin-mediated inhibition of TOR signaling. As TOR signaling is also required for heart restoration after cryoinjury, we compared transcriptomes of hyperplastic and regenerating ventricles. Both conditions were associated with upregulation of cardiomyocyte dedifferentiation...

... in adult zebrafish. Using a newly generated CreER T2 transgenic line, we show that the cells directing expression of the bridging glial marker ctgfa give rise to regenerating glia after injury, with negligible contribution to either neuronal or oligodendrocyte lineages. A 1 kb sequence upstream...