... technique combining Easi-CRISPR with in utero electroporation allows efficient and rapid visualization of endogenous proteins in embryonic brain tissue. CRISPR Brain development Radial glia Neurons In utero electroporation FMRP National Institutes of Health http://dx.doi.org...

... not be confused with the transient cell type of the same name in the developing mammalian cortex ( Villalba et al., 2021 ) ( Fig. 1 B,C). Radial glia in zebrafish are likely direct descendants of ventricular cells during development and increase proliferation and neurogenesis after brain injury ( Barbosa et al...

...Li He; Jennifer Jones; Weiguo He; Bryan C. Bjork; Jiayu Wen; Qi Dai ABSTRACT Radial glia (RG) in the neocortex sequentially generate distinct subtypes of projection neurons, accounting for the diversity and complex assembly of cortical neural circuits. Mechanisms that drive the rapid and precise...

... characterized. Here, we combine experiments of radial glia cultures with numerical models and a branching process theoretical formalism to show that fibroblast growth factor 2 has a triple effect by simultaneously increasing the growth fraction, promoting symmetric divisions and shortening the length...

... detection in the mammalian retina . Curr. Biol.   15 , 1099 - 1107 . 10.1016/j.cub.2005.05.053 Siqueira , M. , Francis , D. , Gisbert , D. , Gomes , F. C. A. and Stipursky , J. ( 2018 ). Radial glia cells control angiogenesis in the developing cerebral cortex through TGF...

... nervous system injury. However, the cellular identity and the biological properties of adult newborn neurons are elusive for most brain areas. Here, we have used short-term lineage tracing of radial glia progeny to prospectively isolate newborn neurons from the her4.1 + radial glia lineage...

... immediately outside the neuroepithelium causes basal overshooting of progenitor cell nuclei/somata, disturbing their cell cycle and apical nucleokinesis, suggesting the importance of neuron-progenitor physical interactions. Interkinetic nuclear migration Radial glia Ventricular zone Pseudostratified...

... conditional lineage tracing we demonstrate that cerebellar regeneration depends on the availability of specific stem cells. Radial glia-like cells are thought to be the predominant stem cell type in homeostasis and after injury. However, we find that radial glia-like cells play a minor role in adult...

... development, the Arp2/3 actin branching complex regulates process formation and the maintenance of radial glial cell polarity, as well as affecting neuronal migration. Arp2/3 complex Actin Cortical development Neurogenesis Neuronal migration Radial glia Mouse National Institutes of Health...

...Robert N. Duncan; Yuanyuan Xie; Adam D. McPherson; Andrew V. Taibi; Joshua L. Bonkowsky; Adam D. Douglass; Richard I. Dorsky The vertebrate hypothalamus contains persistent radial glia that have been proposed to function as neural progenitors. In zebrafish, a high level of postembryonic...

... have been identified, namely short neuronal precursors located in the VZ ( Gal et al., 2006 ; Hartfuss et al., 2001 ; Stancik et al., 2010 ), as well as basal RGCs, which share radial glia characteristics although they reside in the SVZ ( Borrell and Gotz, 2014 ; Pilz et al., 2013 ; Shitamukai et...

... ( [email protected] ) Competing interests The authors declare no competing financial interests. 10 4 2014 1 9 2014 © 2014. Published by The Company of Biologists Ltd 2014 Commissure Radial glia Glial wedge Indusium griseum Guidepost neurons Nervous system...

... inactivation in mice, we show that CCM3 has both cell autonomous and cell non-autonomous functions in neural progenitors and is specifically required in radial glia and newly born pyramidal neurons migrating through the subventricular zone, but not in those migrating through the cortical plate. Loss of CCM3...

... for correspondence ( [email protected] ) Competing interests statement The authors declare no competing financial interests. 7 3 2013 © 2013. Published by The Company of Biologists Ltd 2013 Ephrin Cell adhesion Integrin Arf6 Exencephaly Radial glia Mouse...

.... Published by The Company of Biologists Ltd Transcriptional regulator Radial glia Cortex development Mouse During organ formation, cell proliferation and differentiation have to be tightly controlled and coordinated. This is of particular relevance in the CNS, as regulation of neuron...

... declare no competing financial interests. 21 9 2011 © 2011. Radial glia Asymmetric cell division Neurogenesis Spag5 The mammalian neocortex develops from a pseudostratified neuroepithelium by progressive cell divisions of neuroepithelial cells and radial glia ( Götz...

... of reelin on neuronal migration. Here, we show that reelin gain-of-function causes a higher expression of Notch target genes in radial glia and accelerates the production of both neurons and intermediate progenitor cells. Converse alterations correlate with reelin loss-of-function, consistent with reelin...

... that maintained an astro/radial glia identity. These, in turn, gave rise to symmetrically and fast-dividing cells that lost glial hallmarks, but had not yet acquired neuronal features. The number of amplifying divisions was limited to a maximum of five in this system. Moreover, we found that cell growth...

...Yukako Yokota; Tae-Yeon Eom; Amelia Stanco; Woo-Yang Kim; Sarada Rao; William D. Snider; E. S. Anton Polarized radial glia are crucial to the formation of the cerebral cortex. They serve as neural progenitors and as guides for neuronal placement in the developing cerebral cortex. The maintenance...

... cases, lie at the origin of brain tumours and other diseases. * Authors for correspondence ( [email protected] ; [email protected] ) © 2010. 2010 Drosophila Brain tumours Neural stem cells Radial glia Self-renewal In recent years, tumour cells with stem-like...