..., a process essential for tissue patterning and function. Localized in ovo inhibition in quail embryos of retinoic acid activity followed by single-cell transcriptomics unraveled a comprehensive list of differentially expressed genes relevant to these processes. Importantly, progenitors co-expressed neural...

... interests. 5 9 2022 3 5 2023 © 2023. Published by The Company of Biologists Ltd 2023 Highlighted Article: The characterization of vacuoles in chick and quail hemogenic endothelial cells, and the analysis of aquaporin localization suggest that endothelial cell rounding...

.... Published by The Company of Biologists Ltd 2016 Summary: A new model for understanding the earliest steps of hematopoiesis is based on the in vitro recapitulation of hemogenic endothelium commitment from quail pre-somitic mesoderm. Hemogenic endothelium Hematopoiesis Endothelium Aorta...

... , 4335 - 4347 . Burns , A. J. and Le Douarin , N. M. ( 2001 ). Enteric nervous system development: analysis of the selective developmental potentialities of vagal and sacral neural crest cells using quail-chick chimeras . Anat. Rec.   262 , 16 - 28 . 10.1002/1097-0185(20010101)262:1...

... of sperm extract (SE) or its components led to the development and birth of healthy quail chicks. SE contains three factors – phospholipase Cζ (PLCZ), aconitate hydratase (AH) and citrate synthase (CS) – all of which are essential for full egg activation and subsequent embryonic development. PLCZ induces...

... lineage-tracing studies, we demonstrate that mesothelial progenitors of the intestine are intrinsic to the gut tube anlage. Furthermore, a novel chick-quail chimera model of gut morphogenesis reveals these mesothelial progenitors are broadly distributed throughout the gut primordium and are not derived...

... parallel to ephrin stripes. This behavior can be replicated in a heterologous in vivo system, the hindbrain of the developing quail, in which neural crest cells are directed in streams to the branchial arches and to the forelimb of the developing quail, where presumptive limb myoblasts emigrate from...

... significant progress has been made toward elucidating the embryonic origins of the myocardium, the origins of the endocardium remain unclear. Here, we have identified an endocardium-forming field medial to the cardiac crescent, in a continuum with the endothelial plexus. In vivo live imaging of quail embryos...

... were re-incubated for 16-45 hours. Fertile quail ( Coturnix coturnix Japonica ) and chick ( Gallus gallus ) eggs from commercial sources (Moshav Orot and Moshav Mata) were used in this study. Analysis was restricted to interlimb levels of the axis (somites 21-26). Embryos were staged according...

... ( [email protected] ) Competing interests statement 16 6 2010 © 2010. Muscle development Myogenesis Skeletal Somite Vertebrate Chick Quail The innovation that permitted free movement of the head independently of the body is considered...

... was assayed using Magic Red Caspases 3&7 reagent(Neuromics). To determine whether FOXD3 could repress expression of MITF in avian melanoblasts, cultured quail NC cells were transfected with pFOXD3 or with empty pMES and the cells then labeled with an anti-MITF antibody. In cultures transfected...

... to produce and select ES cell-derived neuroepithelial progenitors and showed that neuroectoderm, rather than mesoderm, may be a source of adipocytes in mES cell-derived cultures. We then used primary and secondary cultures of developing quail neural crest (NC) cells to demonstrate that NC cells are able...

... Epithelial to mesenchymal transition Wnt Quail The neural crest (NC) is a transient population of embryonic progenitors that constitutes the dorsal midline of the early neural tube (NT) and generates a wide variety of derivatives( Le Douarin and Kalcheim,1999 ). The molecular network underlying NC...

... patterning. Development 130 , 4025 -4036. Bally-Cuif, L. and Wassef, M. ( 1994 ). Ectopic induction and reorganization of Wnt-1 expression in quail/chick chimeras. Development 120 , 3379 -3394. Chizhikov, V. V. and Millen, K. J. ( 2004a ). Mechanisms of roof plate formation in the vertebrate...

... state of myoblasts, suppressing their differentiation, while a further branchial arch derived signal, namely Bmp7, is an overall negative regulator of head myogenesis. Fertilised hens' and quails' eggs were obtained from Winter Farm (Royston)and Potter Farm (Woodhurst), and incubated at 38.5°C...

..., functions of these transcription factors in neural crest cells are enhanced by PKA signaling. * Author for correspondence (e-mail: [email protected] ) 24 1 2006 © 2006. 2006 Neural crest Sox9 Slug Snail2 PKA BMP EMT Quail Neural crest...

... to placode inducing agents. Therefore, to unravel the signalling events that generate all sensory placodes it is essential to understand the mechanisms that specify ectodermal cells as PPR. To test this possibility, we grafted quail neural plates (stage 5-6) into the extra-embryonic ectoderm of chick...

... Vasculogenesis Endothelial cells αvβ3 integrin Time-lapse Computational biology Quail This study was designed to understand how cell dynamics contribute to the de novo assembly of an organ – the primordial embryonic vasculature. It is widely assumed that motility-related cellular activities...

... patterning Chick Quail The isthmic organizer (IO), located at the constriction (or isthmus) that marks the mid/hindbrain (MHB) junction of the developing neural tube, is essential for the growth and patterning of the midbrain and anterior hindbrain( Joyner et al., 2000 ; Rhin and Brand, 2001...

...(AB1991, Chemicon International), monoclonal anti-quail axons or quail cells(QN or QCPN, respectively; Developmental Studies Hybridoma Bank), monoclonals 4D5 (anti-Islet1/2), 4H9 (anti-Islet2), and polyclonals anti-Chx10 and anti-Lim3 (all kind gifts of Dr T. Jessell). We also used monoclonals anti-Nkx2.2...