..., a process essential for tissue patterning and function. Localized in ovo inhibition in quail embryos of retinoic acid activity followed by single-cell transcriptomics unraveled a comprehensive list of differentially expressed genes relevant to these processes. Importantly, progenitors co-expressed neural...

... (arrowheads). (D) Immunoelectron microscopy of wild-type quail embryos at E3.5. Endothelial cells in the aortic floor contain large vacuoles (asterisks). A magnified view of the area outlined is shown in the right panel. AQP1 (6 nm gold particles) was localized in the plasma and vacuole membrane (VM). (E,F...

.... Published by The Company of Biologists Ltd 2016 Summary: A new model for understanding the earliest steps of hematopoiesis is based on the in vitro recapitulation of hemogenic endothelium commitment from quail pre-somitic mesoderm. Hemogenic endothelium Hematopoiesis Endothelium Aorta...

... enteric nervous system by regulating the composition of the intestinal microenvironment. Enteric nervous system Sonic hedgehog Patched Ptch Epithelium Extracellular matrix Enteric neural crest cells Hirschsprung disease Chick Quail Mouse Gut development relies on interactions among...

... of sperm extract (SE) or its components led to the development and birth of healthy quail chicks. SE contains three factors – phospholipase Cζ (PLCZ), aconitate hydratase (AH) and citrate synthase (CS) – all of which are essential for full egg activation and subsequent embryonic development. PLCZ induces...

... lineage-tracing studies, we demonstrate that mesothelial progenitors of the intestine are intrinsic to the gut tube anlage. Furthermore, a novel chick-quail chimera model of gut morphogenesis reveals these mesothelial progenitors are broadly distributed throughout the gut primordium and are not derived...

... parallel to ephrin stripes. This behavior can be replicated in a heterologous in vivo system, the hindbrain of the developing quail, in which neural crest cells are directed in streams to the branchial arches and to the forelimb of the developing quail, where presumptive limb myoblasts emigrate from...

... significant progress has been made toward elucidating the embryonic origins of the myocardium, the origins of the endocardium remain unclear. Here, we have identified an endocardium-forming field medial to the cardiac crescent, in a continuum with the endothelial plexus. In vivo live imaging of quail embryos...

... Dermis Desmin Epithelium Lineage segregation Myotome Somite ZO-1 Quail Chick The dermomyotome (DM) is a transient epithelium formed during somite development that gives rise to myofibers, mitotic muscle progenitors, dorsal dermis, and also the smooth muscle...

... ( [email protected] ) Competing interests statement 16 6 2010 © 2010. Muscle development Myogenesis Skeletal Somite Vertebrate Chick Quail The innovation that permitted free movement of the head independently of the body is considered...

... were dissected from stage 12-13 Japanese quail ( Coturnix japonica ) embryos according to established protocols ( Kos et al.,2001 ). In some experiments, the neural tubes were removed and replated after 24 hours and cultured for an additional 48-72 hours. In other experiments, clusters of melanoblasts...

... to produce and select ES cell-derived neuroepithelial progenitors and showed that neuroectoderm, rather than mesoderm, may be a source of adipocytes in mES cell-derived cultures. We then used primary and secondary cultures of developing quail neural crest (NC) cells to demonstrate that NC cells are able...

... to mesenchymal transition Wnt Quail The neural crest (NC) is a transient population of embryonic progenitors that constitutes the dorsal midline of the early neural tube (NT) and generates a wide variety of derivatives( Le Douarin and Kalcheim,1999 ). The molecular network underlying NC specification...

... organizer Roof plate FGF8 Follistatin Activin BMP7 GDF7 Chick Quail References Adams, K. A., Maida, J. M., Golden, J. A. and Riddle, R. D. ( 2000 ). The transcription factor Lmx1b maintains Wnt1 expression within the isthmic organizer. Development 127 , 1857 -1867. Akai, J...

... 5 2006 © 2006. 2006 Chick Quail Embryo Head mesoderm Skeletal muscles Eye muscles Lateral rectus Branchiomeric muscles Mandibular arch Jaw muscles Lbx1 Paraxis En2 Myf5 MyoR Fgf8 Bmp7 Vertebrate skeletal muscles are the basis for all voluntary...

..., functions of these transcription factors in neural crest cells are enhanced by PKA signaling. * Author for correspondence (e-mail: [email protected] ) 24 1 2006 © 2006. 2006 Neural crest Sox9 Slug Snail2 PKA BMP EMT Quail Neural crest...

.... A. and Hemmati-Brivanlou,A. ( 1999 ). Pax6 induces ectopic eyes in a vertebrate. Development 126 , 4213 -4222. Couly, G. F. and Le Douarin, N. M. ( 1985 ). Mapping of the early neural primordium in quail-chick chimeras. I. Developmental relationships between placodes, facial ectoderm, and prosencephalon...

... and found to be reduced in the presence of an αvβ3 integrin inhibitor. These dynamic cell motility data result in new hypotheses regarding primordial endothelial cell behavior during embryonic vasculogenesis. Vasculogenesis Endothelial cells αvβ3 integrin Time-lapse Computational biology Quail...

... patterning Chick Quail The isthmic organizer (IO), located at the constriction (or isthmus) that marks the mid/hindbrain (MHB) junction of the developing neural tube, is essential for the growth and patterning of the midbrain and anterior hindbrain( Joyner et al., 2000 ; Rhin and Brand, 2001...

... antibody and Fast Red development followed. All reagents used for in situ procedures were from Roche Molecular Biochemicals, except for Fast Red (Sigma). In grafting experiments, the correct graft position and integration was ensured by immunostaining with a monoclonal antibody to quail cells (QCPN...