...) is required for precise orientation of a mitotic spindle in response to a Wnt cue. We find that SDN-1 is the predominant heparan sulfate (HS) proteoglycan in the early C. elegans embryo, and that loss of HS biosynthesis or of the SDN-1 core protein results in misorientation of the spindle of the ABar...

...Cammon B. Arrington; H. Joseph Yost One of the first steps in zebrafish heart and gut organogenesis is the migration of bilateral primordia to the midline to form cardiac and gut tubes. The mechanisms that regulate this process are poorly understood. Here we show that the proteoglycan syndecan 2...

...Mauricio Cortes; Alexis T. Baria; Nancy B. Schwartz In contrast to the functional role of heparan sulfate proteoglycans(HSPGs), the importance of chondroitin sulfate proteoglycans (CSPGs) in modulating signaling pathways involving hedgehog proteins, wingless-related proteins and fibroblast growth...

... epithelium, is a key determinant of embryonic dorsoventral polarity, suggesting that Pipe-mediated sulfation of a heparan sulfate proteoglycan provides a spatial cue for dorsoventral axis formation. We used several approaches to investigate this possibility in the work described here. We determined...

...Daniela Perissinotto; Paola Iacopetti; Isabella Bellina; Roberto Doliana; Alfonso Colombatti; Zoé Pettway; Marianne Bronner-Fraser; Tamayuki Shinomura; Koji Kimata; Matthias Mörgelin; Jan Löfberg; Roberto Perris ABSTRACT It has been proposed that hyaluronan-binding proteoglycans play an important...

... that survived TGF-α-PE were nevertheless killed by HB-EGF-PE. HB-EGF-PE toxicity was partially mediated by cell surface heparan sulfate proteoglycans (HSPG), since a peptide corresponding to the heparin-binding domain of HB-EGF as well as heparitinase treatment protected the blastocysts from the toxic effects...

... through associations with heparan sulfate proteoglycans. Neuregulin accumulation in the synaptic basal lamina of neuromuscular junctions occurred significantly later, coincident with a reorganization of muscle extracellular matrix resulting in a relative concentration of heparan sulfate proteoglycans...

...Udo Häcker; Xinhua Lin; Norbert Perrimon ABSTRACT We have identified and characterized a Drosophila gene, which we have named sugarless , that encodes a homologue of vertebrate UDP-glucose dehydrogenase. This enzyme is essential for the biosynthesis of various proteoglycans, and we find...

... proteoglycan that mediates agrin activity. To further test this hypothesis, we have compared the ability of differentially active agrin isoforms to interact with a model component of proteoglycans, heparin, as well as with the putative proteoglycan α-dystroglycan. We demonstrate that an alternately spliced...

...Nobuaki Maeda; Masaharu Noda ABSTRACT 6B4 proteoglycan/phosphacan is one of the major phosphate-buffered saline-soluble chondroitin sulfate proteoglycans of the brain. Recently, this molecule has been demonstrated to be an extracellular variant of the proteoglycan-type protein tyrosine phosphatase...

... with homology to a family of Glypican-related integral membrane proteoglycans. These heparan sulfate-containing proteins are attached to the external leaflet of the plasma membrane via a glycosylphosphatidylinositol linkage. Heparan sulfate proteoglycans may serve as co-receptors for a variety of secreted...

... the archen-teron lumen open against the internal pressure of the blastocoel. Thus, this model could be extended to explain some instances of lumen formation by epithelial cells. Fig. 6. Whole-mount immunocytochemical localization of chondroitin sulfate proteoglycan in normal and Bromo-A23187...

...Akiko Nishiyama; Kimberlee J. Dahlin; William B. Stallcup ABSTRACT NG2 is a chondroitin sulfate proteoglycan previously found to be expressed by glial progenitor cells of the O2A lineage. We have examined the expression of NG2 in the developing rat limb by immunohistochemistry and northern blot...

...H. Joseph Yost The heart of any vertebrate is formed from an apparently symmetric cardiac tube that loops consistently in the same direction along the left-right axis of the embryo. In the amphibian Xenopus laevis , inhibition of proteoglycan synthesis by p-nitrophenyl- β -D-xylopyranoside during...

...Lauren E. Swenarchuk; Shashikant Champaneria; M. John Anderson To identify mechanisms that regulate the formation of the neuromuscular junction, we examined the cellular origin of a heparan sulfate proteoglycan (HSPG) that becomes highly concentrated within the synaptic cleft during the initial...

... isolated with an intact basal lamina that lay down in vitro ∼3 /im-thick stromas of organized fibrils similar to that seen in vivo . This experimental system has yielded two unexpected results. First, the amount of collagen and proteoglycans produced by such epi-thelia is not dependent on whether its...

...David A. Carrino; Uri Oron; David G. Pechak; Arnold I. Caplan Previous work from this laboratory involved the characterization of a large chondroitin sulphate proteoglycan unique to chick skeletal muscle. This proteoglycan is synthesized by embryonic skeletal muscle both in ovo and in culture...