... the regulatory mechanisms in heart morphogenesis are not well understood, increasing attention has focused on the homeodomain proteins, which are generally linked to morphogenetic processes. The homeodomain containing gene Gax has been shown to be expressed in heart and smooth muscle tissues. In this study...

...Erik-Jan Dekker; Marie-Josée Vaessen; Claudia van den Berg; Ans Timmermans; Sue Godsave; Tjadine Holling; Pieter Nieuwkoop; Ad Geurts van Kessel; Antony Durston ABSTRACT We have isolated the first Xenopus laevis cDNA coding for a cellular retinoic acid binding protein ( xCRABP ). xCRABP contains...

...Kirsi Sainio; Scott F. Gilbert; Eero Lehtonen; Miyuki Nishi; Nalin M. Kumar; Norton B. Gilula; Lauri Saxén ABSTRACT The expression of three gap junction (GJ) proteins, α 1 (Cx43), β 1 (Cx32), and β 2 (Cx26), and their transcripts were examined during the ontogeny of the mouse and rat kidney...

... and mesoderm. A single copy of a putative finger motif, of the ‘CCHC’ type, is located near the carboxyl terminus. One or two copies of similar sequence motifs are found in the nucleocapsid protein of retroviruses where they are involved in protein-RNA interactions, and in cellular nucleic acid binding protein...

... and immunofluorescence histochemistry on selected stages of embryonic chick tibiotarsi. The data show that mRNA for type X collagen appears at or about the time that we detect the first appearance of the protein. This result is incompatible with translational regulation, which would require accumulation of the mRNA...

... and Medicine, University of Melbourne, Parkville, Victoria 3052, Australia © 1990 by Company of Biologists 1990 insulin-like growth factors IGF-II receptor rat embryo mannose-6-phosphate mRNA protein Insulin-like growth factors (IGFs) are peptides that have been implicated in embryonic...

... studies have been carried out by gene transfer, and are supported by in vitro analyses of DNA-protein interactions in the regulatory regions of two genes that are expressed in a lineage-specific manner. Expression of both of these markers of cell fate specification is controlled by diffusible DNA-binding...

... fluorescence immunocytochemistry to examine the distribution in growth cones, their filopodia and lamellipodia of several actin-associated proteins and nerve cell adhesion molecules. The leading margins of chick dorsal root ganglion nerve growth cones and their protrusions stain strongly for f-actin, filamin...

... segment. We report here several observations relating to the role and disposition of the engrailed protein during the embryonic stages that precede cellularization. We demonstrate that in embryos mutant for the engrailed gene, there were characteristic morphological abnormalities as early as the 6th...

...J. W. Mahaffey; R. J. Diederich; T. C. Kaufman Antibodies that specifically recognize proteins encoded by the homeotic genes: Sex combs reduced, Deformed, labial and proboscipedia , were used to follow the distribution of these gene products during embryogenesis. The position of engrailed...

...S. K. Howlett; S. C. Barton; M. L. Norris; M. A. H. Surani 1-, 2- and 4-cell mouse embryos were labelled with [ 35 S]methionine and the newly synthesized proteins from isolated nuclei were compared with those from cytoplasts and total embryos. There were distinct subsets of translation products...

... of the diencephalon. With the exception of the oral cavity, sites of immunoreactivity for serotonin-binding protein were identified in the mesenchyme adjacent to these sites. This mesenchyme consists of ectodermally derived neural crest cells, which are known to receive inductive influences from the epithelia...

... specificity. Analysis of amino acid sequences deduced from cDNA encoding these molecules showed that they are integral membrane proteins of 723–748 amino acids long and share common sequences; similarity in the sequences between subclasses is in a range of 50–60 % when compared within a single animal species...

...Dana R. Crawford; Joel D. Richter ABSTRACT Monoclonal antibodies directed against an RNA-binding protein from Xenopus oocytes were used to immunoselect messenger ribonucleoprotein (mRNP) particles. RNA was extracted from both the immunoselected and nonselected fractions and was used to direct...

...S. F. Godsave; B. H. Anderton; C. C. Wylie ABSTRACT Antibodies against various intermediate filament proteins have been used to follow cell differentiation in the early Xenopus embryo. Three new monoclonal antibodies against Xenopus cytokeratins raised against Triton-insoluble material from...

...R. M. Moor; I. M. Crosby ABSTRACT The regulation of the cell cycle during the transition from prophase to metaphase I was studied by analysing protein changes and introducing protein blocks during the transition phase. The results show that the progression to metaphase in ovine oocytes is totally...

... an opposite trend in that it increased over 12 h by nearly 30-fold. In view of these results experiments were conducted in embryos and yolk sacs cultured in separate bottles. Radioimmunoprecipitation for transferrin revealed that there was synthesized protein in the yolk sac which then decreased...

... macromolecules, its abundant proteins and mRNAs were identified and compared to those present in the remainder of the egg. This was accomplished by exploiting a newly developed method for the mass fractionation of yellow crescents which is based on the presence of a unique cytoskeletal domain in the yellow...