... here enables mosaic screens in the context of gene knockdown or overexpression by automatically generating a reliable ratio of mutant to wild-type tissue in a developmentally controlled manner. CoinFLP-Gal4 generates mosaic tissues composed of clones of which only a subset expresses Gal4. CoinFLP...

...Johannes Bischof; Mikael Björklund; Edy Furger; Claus Schertel; Jussi Taipale; Konrad Basler Overexpression screens are used to explore gene functions in Drosophila , but this strategy suffers from the lack of comprehensive and systematic fly strain collections and efficient methods for generating...

... and rfng transcripts (for MO sequences see Fig. S4A-C in the supplementary material ), and overexpression experiments were performed. In vitro translation of lfng and rfng mRNA, in a rabbit reticulocyte lysate system using 35 S-methionine, could be knocked down by each specific MO (see Fig. S4D-F...

...Stephen J. Gaunt; Deborah Drage; Richard C. Trubshaw To investigate the link between Cdx protein concentration and axial patterning in embryos, we made lines of mice OE1, OE2 and OE4 that overexpress each of the Cdx genes Cdx1 , Cdx2 and Cdx4 ,respectively. The lines carry Cdx transgenes under...

... for the complex role of Pax6 in mammalian corticogenesis. * Author for correspondence (e-mail: [email protected] ) 17 1 2007 © 2007. 2007 Corticogenesis Cre/ loxP Overexpression Pax6 Pax6-5a Progenitor Apoptosis Mouse Pax6 is an evolutionarily conserved transcription...

... of late cortical progenitors specifically, and analysis of PAX77↔wild-type chimeras indicates that the defect is cell autonomous. We analyzed cortical arealization in PAX77 mice and found that, whereas the loss of Pax6 shifts caudal cortical areas rostrally, Pax6 overexpression at levels predicted...

... of As -T gene expression (the ascidian brachyury ) ( Yasuo and Satoh, 1993 , 1994 ; Nakatani and Nishida, 1994 ). Moreover when the As-T gene is overexpressed in whole embryos by synthetic As- T mRNA injection into Halocynthia eggs, the fate of endodermal blastomeres is changed to become notochord...

... in the ventral forebrain, sensory neurons, fin buds and excretory system. The expression pattern of tbx-c differs from that of the other two related genes, tbx-a and tbx-b . The developmental role of tbx-c has been analysed by overexpression of the full-length tbx-c mRNA and a truncated form of tbx-c mRNA, which...

... that constitutively expressed exogenous neuropilin, then produced chimeras using these ES cell clones. The chimeras overexpressed neuropilin and were embryonic lethal. The chimeric embryos exhibited several morphological abnormalities; excess capillaries and blood vessels, dilation of blood vessels, malformed hearts...

..., the hsfzI transgene provided almost complete rescue of a null fz mutation, indicating that the protein encoded by this cDNA can fulfill both fz functions. Overexpression of the hsfzI transgene resulted in two distinct tissue polarity phenotypes depending on the time of heat shock. * Author...