... to the oosome, they show unusual localization: Tudor protein forms a shell encapsulating the embryonic oosome, while small Oskar/Vasa/Aubergine granules coalesce interiorly. Wasp Vasa itself is unusual since it has an alternative splice form that includes a previously unreported nucleoporin-like phenylalanine...

..., which induces a microtubule reorganisation that localises bicoid and oskar mRNAs. Here we show that oocyte polarity requires Slmb, the substrate specificity subunit of the SCF E3 ubiquitin ligase that targets proteins for degradation. The Par-6/aPKC complex is ectopically localised to the posterior...

... poor fecundity. In addition, mutant egg chambers exhibit an arrest at the previtellogenic stage. D-EndoB displayed a crescent localization at the oocyte posterior pole in an Oskar-dependent manner; however, it did not contribute to pole plasm assembly. D-EndoB was found to partially colocalize...

...Kristina S. Sinsimer; Roshan A. Jain; Seema Chatterjee; Elizabeth R. Gavis Asymmetric mRNA localization is an effective mechanism for establishing cellular and developmental polarity. Posterior localization of oskar in the Drosophila oocyte targets the synthesis of Oskar to the posterior, where...

...Ritsuko Suyama; Andreas Jenny; Silvia Curado; Wendy Pellis-van Berkel; Anne Ephrussi During Drosophila oogenesis, oskar mRNA is transported to the posterior pole of the oocyte, where it is locally translated and induces germ-plasm assembly. Oskar protein recruits all of the components necessary...

...Tsubasa Tanaka; Akira Nakamura Cell fate is often determined by the intracellular localization of RNAs and proteins. In Drosophila oocytes, oskar ( osk ) RNA localization and the subsequent Osk synthesis at the posterior pole direct the assembly of the pole plasm, where factors for the germline...

... spherical bodies from smaller precursors. Several polar granule components, both protein and RNA, have been identified. One of these, the protein Oskar, acts to initiate granule formation during oogenesis and to recruit other granule components. To investigate whether Oskar has a continuing role...

...Andreas Jenny; Olivier Hachet; Péter Závorszky; Anna Cyrklaff; Matthew D. J. Weston; Daniel St Johnston; Miklós Erdélyi; Anne Ephrussi The Drosophila maternal effect gene oskar encodes the posterior determinant responsible for the formation of the posterior pole plasm in the egg, and thus...

..., and found that the gene is essential for viability. During oogenesis, ik2 is required in an NF-κB-independent process that is essential for the localization of oskar and gurken mRNAs; as a result, females that lack ik2 in the germline produce embryos that are both bicaudal and ventralized. The abnormal RNA...

... also occurs in oskar mutants and other segmentation mutants. In hid bicoid double mutants, mis-specified cells in the presumptive head and thorax survive and continue to develop, but they are transformed to adopt a different cell fate. We provide evidence that the terminal torso signaling pathway...

...-10A, while pattern formation determinants such as oskar mRNA are being localized and anchored at specific sites on the cortex. Then fast well-ordered streaming begins during stage 10B, just before nurse cell cytoplasm is dumped into the oocyte. We report that the plus-end-directed microtubule motor...

... that vls encodes a divergent WD domain protein and that the three available EMS-induced point mutations cause premature stop codons in the vls ORF. We have generated a null allele that has a stronger phenotype than the EMS mutants. The vls null mutant shows that vls + is required for high levels of Oskar...

... and dorsoventral axes of the Drosophila melanogaster embryo are determined by the activities of localized maternal gene products. At the posterior pole of the oocyte, Oskar directs the assembly of the pole plasm,and is thus responsible for formation of abdomen and germline in the embryo. Tight restriction of oskar...

...Sophie G. Martin; Vincent Leclerc; Katie Smith-Litière; Daniel St Johnston The anteroposterior axis of Drosophila is defined during oogenesis, when the polarisation of the oocyte microtubule cytoskeleton directs the localisation of bicoid and oskar mRNAs to the anterior and posterior poles...

...Nathalie F. Vanzo; Anne Ephrussi Localization of the maternal determinant Oskar at the posterior pole of Drosophila melanogaster oocyte provides the positional information for pole plasm formation. Spatial control of Oskar expression is achieved through the tight coupling of mRNA localization...

...Rita Sinka; Ferenc Jankovics; Kálmán Somogyi; Tamás Szlanka; Tamás Lukácsovich; Miklós Erdélyi Embryonic germ cell formation and abdomen development in Drosophila requires localisation and site specific translation of oskar mRNA in the posterior part of the oocyte. Targeting of oskar...

... © 2002. 2002 Oogenesis Cell polarity Membrane trafficking Membrane recycling oskar mRNA localization Microtubule plus ends Drosophila Rab11 Fig. 1. rab11 cloning and genetics. (A) Organization of the rab11 locus. The top line shows the 6.3 kb genomic rescuing...

...Adam N. Harris; Paul M. Macdonald In Drosophila oocytes, activation of Oskar translation from a transcript localized to the posterior pole is an essential step in the organization of the pole plasm, specialized cytoplasm that contains germline and abdominal body patterning determinants. Oskar...

...: Exelixis, 170 Harbor Avenue, PO Box 511, S. San Francisco, CA 94083-0511, USA 21 12 2001 07 02 2001 © 2001 by Company of Biologists 2001 Drosophila oogenesis Merlin gurken bicoid oskar TGFα mRNA localisation Oocyte microtubules Embryonic axis formation Tumour...

[email protected] ) 30 08 2000 24 10 2000 © 2000 by Company of Biologists 2000 Drosophila encore gurken oskar Karyosome Oogenesis Drosophila oogenesis can be used as a system in which to analyze the control of cell division because the germline mitoses that give rise...