...Maëlys Loh; Fred Bernard; Antoine Guichet ABSTRACT Microtubules and their associated motors are important players in nucleus positioning. Although nuclear migration in Drosophila oocytes is controlled by microtubules, a precise role for microtubule-associated molecular motors in nuclear migration...

... attributed. Summary: A nanobody-based method, NaNuTrap, allows early in vivo cell nuclei labelling independently of the maturation time of the fluorescent protein and for transforming of cytoplasmic GFP-expressing lines into nuclear-labelled lines. Nanobody Nucleus GFP maturation Live...

.... ( 2018 ). Dealing with the nucleus during cell migration . Curr. Opin. Cell Biol. 50 , 35 - 41 . 10.1016/j.ceb.2018.01.014 DasGupta , R. , Boutros , M. and Perrimon , N. ( 2005 ). Drosophila Wnt/Fz pathways . Science's STKE 2005 , cm5 . 10.1126/stke.2832005cm5...

... clustering of target genes to form consolidated hubs ( De Wit et al., 2013 ). In line with the observed locational stability of transcription factor binding hotspots, the movement of chromatin fibers within the nucleus is slow due to their size ( Dion and Gasser, 2013 ). Therefore, DNA can be sufficiently...

...You Kure Wu; Hiroki Umeshima; Junko Kurisu; Mineko Kengaku ABSTRACT Nuclear migration of newly born neurons is essential for cortex formation in the brain. The nucleus is translocated by actin and microtubules, yet the actual force generated by the interplay of these cytoskeletons remains elusive...

... following enucleation. Consistent with this conclusion, the increased efficiency of reprogramming when using donor nuclei from embryonic cells could be explained, at least in part, by reintroduction of embryonic nuclear factors along with the donor nucleus. By contrast, cell cycle synchrony between...

... this transport takes place. We have identified a centriole-containing centrosome close to the oocyte nucleus. Remarkably, the centrosomal components, γ-tubulin and Drosophila pericentrin-like protein also strongly accumulate at the periphery of this nucleus. MT polymerization after cold-induced disassembly...

... not stimulate meiotic resumption. The ability to cause oocyte activation was specific to nuclei transferred from fertilized embryos as nuclei from parthenogenetic embryos or cytoplasts from fertilized or parthenogenetic embryos did not induce activation. Nucleus-induced oocyte activation was associated...

...C. Aimar ABSTRACT The role of the cytoplasm and nucleus in the control of the length of the division cycle was investigated in Pleurodeles waltl eggs. Injection of spermine into enucleated eggs showed that the ability to cleave was not restricted just to the period of normal cytokinesis (T = 1·0...

... nuclear lamins nucleus mouse preimplantation immunofluorescence The nuclear lamina is a proteic meshwork associated with nuclear pores and apposed to the inner nuclear membrane. Together with the internal scaffold (or matrix), it constitutes the nucleoskeleton. In most mammalian cells...

... into the cytoplasm of mitotic PtK2-cell, the newly formed daughter nuclei show intense staining when examined by immunofluorescence microscopy whereas adjacent cells are totally negative ( Fig. 6B ). When the same antibodies are injected into the nucleus of a Pleurodeles oocyte, the lampbrush chromosome loops...

... 1986 by Company of Biologists 1986 ascidian embryo maternal mRNA proteins determinant alkaline phosphatase actin nucleus Styela plicata . This paper reviews the class of cytoplasmic determinants that function independently of embryonic gene expression in ascidian embryos...