...Miguel Robles-Garcia; Chloë Thimonier; Konstantina Angoura; Ewa Ozga; Heather MacPherson; Guillaume Blin ABSTRACT Notochord progenitors (NotoPs) represent a scarce yet crucial embryonic cell population, playing important roles in embryo patterning and eventually giving rise to the cells that form...

... the etiology remains elusive. The notochord is pivotal for vertebrae development, but its role in CS is still understudied. Here, we generated a zebrafish knockout of ptk7a , a planar cell polarity (PCP) gene that is essential for convergence and extension (C&E) of the notochord, and detected congenital...

...Dennis Schifferl; Manuela Scholze-Wittler; Alba Villaronga Luque; Milena Pustet; Lars Wittler; Jesse V. Veenvliet; Frederic Koch; Bernhard G. Herrmann ABSTRACT The node and notochord are important signaling centers organizing the dorso-ventral patterning of cells arising from neuro-mesodermal...

... and that they are later incorporated in the anterior notochord. Our analysis of notochord formation in wild type and in embryos in which FGF signalling is inhibited also reveals that amphioxus anterior notochord presents transient prechordal plate features. Altogether, our results give insight into how changes in FGF...

...Dennis Schifferl; Manuela Scholze-Wittler; Lars Wittler; Jesse V. Veenvliet; Frederic Koch; Bernhard G. Herrmann ABSTRACT The node-streak border region comprising notochord progenitor cells (NPCs) at the posterior node and neuro-mesodermal progenitor cells (NMPs) in the adjacent epiblast...

... across different organisms. Here, we apply a quantitative approach to learn how the notochord forms during the development of amphioxus: a basally branching chordate. Using a single-cell morphometrics pipeline, we quantify the geometries of thousands of amphioxus notochord cells, and project them...

...Susannah B. P. McLaren; Benjamin J. Steventon ABSTRACT How force generated by the morphogenesis of one tissue impacts the morphogenesis of other tissues to achieve an elongated embryo axis is not well understood. The notochord runs along the length of the somitic compartment and is flanked...

...Wendy M. Reeves; Kotaro Shimai; Konner M. Winkley; Michael T. Veeman ABSTRACT The notochord is a defining feature of the chordates. The transcription factor Brachyury (Bra) is a key regulator of notochord fate but here we show that it is not a unitary master regulator in the model chordate Ciona...

...Shiny Shengzhen Guo; Tiffany Y. K. Au; Sarah Wynn; Attila Aszodi; Danny Chan; Reinhard Fässler; Kathryn S. E. Cheah ABSTRACT The notochord drives longitudinal growth of the body axis by convergent extension, a highly conserved developmental process that depends on non-canonical Wnt/planar cell...

..., and highlight open questions. We specifically focus on the processes of notochord development and cerebrospinal fluid physiology, and how defects in those processes may lead to scoliosis. * Authors for correspondence ( [email protected] , [email protected] ) Competing interests...

...-mineralized spine with gradually fusing vertebral body precursors (centra). However, the underlying cellular mechanisms remain incompletely understood. Here, we show that cells of the notochord epithelium named chordoblasts are sensitive to RA signaling. Chordoblasts are uniformly distributed along...

...Wendy M. Reeves; Yuye Wu; Matthew J. Harder; Michael T. Veeman The notochord of the ascidian Ciona consists of only 40 cells, and is a longstanding model for studying organogenesis in a small, simple embryo. Here, we perform RNAseq on flow-sorted notochord cells from multiple stages to define...

... germ layer plasticity, these cells never give rise to midline tissues of the notochord, floor plate and dorsal endoderm, raising the question of whether midline tissues also arise from basal posterior progenitors after gastrulation. We show in zebrafish that local posterior signals specify germ layer...

...Magdalena Stasiulewicz; Shona D. Gray; Ioanna Mastromina; Joana C. Silva; Mia Björklund; Philip A. Seymour; David Booth; Calum Thompson; Richard J. Green; Emma A. Hall; Palle Serup; J. Kim Dale Notochord-derived Sonic Hedgehog (Shh) is essential for dorsoventral patterning of the overlying neural...

... that the notochord plays an instructive role in segmental patterning, alongside the somites, and contributes to mineralization. By clarifying the evolutionary relationship between centra and arches, and their varying modes of skeletal mineralization, we can better appreciate the detailed mechanisms that regulate...

...Elsa Denker; Ivonne M. Sehring; Bo Dong; Julien Audisso; Birthe Mathiesen; Di Jiang Regulation of lumen growth is crucial to ensure the correct morphology, dimensions and function of a tubular structure. How this is controlled is still poorly understood. During Ciona intestinalis notochord...

... A-P axis elongation. Both genes regulate trunk notochord extension through PCP-controlled CE of notochord cells, establishing a role for Wnt11 in mammalian PCP. We show that Wnt5a and Wnt11 are required for proper patterning of the neural tube and somites by regulating notochord formation, and provide...

... environmental and genetic variation that might otherwise perturb development and growth. (3) Force production is distributed between neural and mesodermal tissues in the dorsal isolate, and the notochord, a central structure involved in patterning vertebrate morphogenesis, is not required for force production...

...Nicolas Buisson; Cathy Sirour; Nicole Moreau; Elsa Denker; Ronan Le Bouffant; Aline Goullancourt; Thierry Darribère; Valérie Bello Dystroglycan (Dg) is a transmembrane receptor for laminin that must be expressed at the right time and place in order to be involved in notochord morphogenesis...

...Diana Corallo; Alvise Schiavinato; Valeria Trapani; Enrico Moro; Francesco Argenton; Paolo Bonaldo The notochord is a transient and essential structure that provides both mechanical and signaling cues to the developing vertebrate embryo. In teleosts, the notochord is composed of a core of large...